Cell and Tissue Research

, Volume 223, Issue 3, pp 641–657 | Cite as

Effects of experimental hypo-or hypernatremia on the fine structure of the pars intermedia of the murine pituitary

A morphometric study
  • G. Schmitt
  • M. E. Stoeckel
  • M. J. Klein
  • A. Porte
Article
Accepted:

Summary

Quantified ultrastructural observations of the pars intermedia (PI) of the murine hypophysis enable evaluation and kinetic study of relatively fine secretory changes in the gland. Changes in volume of rER and newly formed dense secretory granules (Golgi granules) appear to best translate functional variations in the PI, as shown by the morphological effects of drugs affecting the dopaminergic control of the gland. Our morphometric results show that the PI is stimulated, but only briefly (no longer than 8–12 days), by both salt-loading and Na deprivation. However, the PI displays different secretory patterns in salt-loaded and Na-deprived mice; moreover, bromocriptine, which abolishes PI stimulation in Na-deprived mice, has only a slight inhibitory effect in salt-treated animals. Thus, it appears that the stimulation of the PI under both experimental conditions is triggered by different mechanisms. These results underline the plurifactorial control of the PI and show that the gland may have complex effects on hydromineral regulation.

Key words

Pars intermedia (mouse) Fine structure Morphometry Sodium Dopaminergic control 
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References

  1. Alper RH, Demarest TK, Moore KE (1980) Dehydration selectively increases dopamine synthesis in tuberohypophyseal dopaminergic neurons. Neuroendocrinology 31:112–115Google Scholar
  2. Björklund A, Moore RY, Nobin A, Stenevi V (1973) The organization of tubero-hypophyseal and reticulo-infundibular catecholamine neuron systems in the rat brain. Brain Res 51:171–191Google Scholar
  3. Blaustein MP (1977) Sodium ions, calcium ions, blood pressure regulation, and hypertension: a reassessment and a hypothesis. Am J Physiol 232:C165-C173Google Scholar
  4. Bower A, Hadley ME, Hruby VJ (1974) Biogenic amines and control of melanophore-stimulating hormone release. Science 184:70–72Google Scholar
  5. Burri PH, Hecker H (1979) Stereological study of trypanosoma, a small protozoon. In: Weibel ER (ed) Stereological methods. Academic Press, London, pp 343–348Google Scholar
  6. Cote T, Munemara M, Eskay RL, Kebabian JW (1980) Biochemical identification of the adrenoreceptor and evidence for the involvement of an adenosine 3′5′-monophosphate system in the β-adrenergically induced release of α-melanocyte-stimulating hormone in the intermediate lobe of the rat pituitary gland. Endocrinology 107:108–115Google Scholar
  7. Cyrkowicz A, Traczyk WZ (1975) Effect of intravenous and intracarotid injections of hypertonic fluids on the release of melanocyte-stimulating hormone from the rat posterior pituitary lobe incubated in situ. J Endocrinol 66:85–91Google Scholar
  8. Domer RR, Sankar R, Kastin AJ (1981) Effects of α-MSH on the blood-brain barrier. Fed Proc 40:275Google Scholar
  9. Duchen LW (1962) The effects of ingestion of hypertonic saline on the pituitary gland of the rat: a morphological study of the pars intermedia and posterior lobe. J Endocrinol 25:161–168Google Scholar
  10. Dupont A, Kastin AJ, Labrie F, Pelletier G, Puviani R, Sehally AV (1975) Distribution of radioactivity in the organs of the rat and mouse after injection of (125I) α-melanocyte-stimulating hormone. J Endocrinol 64:237–241Google Scholar
  11. Eberle AN (1980) MSH receptors. In: Schulster D, Levitzki A (eds) Cellular receptors for hormones and neurotransmitters. Wiley J and sons Ltd, New York, pp 219–231Google Scholar
  12. Eipper BA, Mains RE (1980) Structure and biosynthesis of pro-ACTH/endorphin and related peptides. Endocrine Reviews 1:1–27Google Scholar
  13. Glickman JA, Carson GD, Challis JRG (1979) Differential effects of synthetic adrenocorticotropin 1–24 and α-melanocyte-stimulating hormone on adrenal function in human and sheep fetuses. Endocrinology 104:34–39Google Scholar
  14. Howe A, Thody AJ (1970) The effect of ingestion of hypertonic saline on the melanocyte-stimulating hormone content and histology of the PI of the rat pituitary gland. J Endocrinol 46:201–208Google Scholar
  15. Hradec J, Horkyk (1979) Natriuretic and kaliuretic effect of melanocyte-stimulating hormones in hamsters. Endocrinologia Experimentalis 13:145–152Google Scholar
  16. Itturiza FC, Levitin H, Estivariz F (1973) A new extrapigmentary effect of alpha MSH. Promotion of sodium uptake in the heart of toads and rats. Horm Metab Res 5:143–144Google Scholar
  17. Kastin AJ (1967) MSH and vasopressin activities in pituitaries of rats treated with hypertonic saline. Fed Proc 26:255Google Scholar
  18. Kobayashi Y (1974) Quantitative and electron microscopic studies on the pars intermedia of the hypophysis. III. Effect of short-term administration of a sodium deficient diet on the pars intermedia of mice. Cell Tissue Res 154:321–327Google Scholar
  19. Kobayashi Y, Takema M (1976) A morphometric study on pars intermedia of the hypophysis during impairment of the renin-angiotensin-aldosterone system in sodium depleted mice. Cell Tissue Res 168:153–159Google Scholar
  20. Laragh JH, Sealey JE (1973) The renin-angiotensin-aldosterone hormonal system and regulation of sodium, potassium, and blood pressure homeostasis. Handbook Physiol Sect 8:831–908Google Scholar
  21. Legait E, Petter F, Legait H (1966) Recherches sur le lobe intermédiaire de l'hypophyse de quelques rongeurs africains. Mammalia 30:337–342Google Scholar
  22. Legait H (1963) Recherches histophysiologiques sur le lobe intermédiaire de l'hypophyse. Thèse, NancyGoogle Scholar
  23. Levitin HP, San Roman J, Itturiza FC (1977) Natriuresis after water-loading in rats bearing transplants of pars intermedia. Experientia 33:682–683Google Scholar
  24. Munemara M, Eskay RL, Kebabian JW (1980) Release of α-melanocyte-stimulating hormone from dispersed cells of the intermediate lobe of the rat pituitary gland: involvement of catecholamines and adenosine 3′,5′-monophosphate. Endocrinology 106:1795–1803Google Scholar
  25. Novales RR (1974) Actions of melanocyte-stimulating hormone. Handbook Physiol Sect 7, IV Part 2:347–366Google Scholar
  26. Orias R, McCann SM (1970) Natriuretic effect of α-MSH in the water-loaded rats. Proc Soc Exp Biol Med 133:469–477Google Scholar
  27. Orias R, McCann SM (1972) Natriuresis induced by alpha-and beta-melanocyte-stimulating hormone (MSH) in rats. Endocrinology 90:700–706Google Scholar
  28. Page RB, Boyd JE, Mulrow PJ (1974) The effects of alpha-melanocyte-stimulating hormone on aldosterone production in the rat. Endocr Res Communications 1:53–62Google Scholar
  29. Roux M, Dubois MP (1976) Immunohistochemical study of the pars intermedia of the mouse pituitary in different experimental conditions. Experientia 32:657–658Google Scholar
  30. Smelik PG, Tilders FJH (1979) Differential control of anterior lobe and intermediate lobe corticotropin (ACTH) secretion in the rat. In: Jones MT, Dallman MF, Gillham B (eds) The interaction within the brain-pituitary-adrenal system. Academic Press, London, pp 17–39Google Scholar
  31. Snedecor GW, Cochran WG (1957) Méthodes statistiques. Association de coordination technique agricole, ParisGoogle Scholar
  32. Soboleva EL (1964) The pars intermedia of the hypophysis during salt loading. Bull Exp Biol Med USSR 55:577–580Google Scholar
  33. Stoeckel ME, Schmitt G, Porte A (1981) Fine structure and cytochemistry of the intermediate lobe of the mammalian pituitary gland under normal and experimental conditions. In: Peptides of the pars intermedia. Pitman Medical, London (Ciba Foundation symposium 81), p 101–127Google Scholar
  34. Taleisnik S, Celis ME, Tomatis ME (1973) Release of melanocyte-stimulating hormone by several stimuli through the activation of a 5-hydroxy-tryptamine mediated inhibitory neuronal mechanism. Neuroendocrinology 13:327–328Google Scholar
  35. Thody AJ (1978) The significance of melanocyte-stimulating hormone (MSH) and the control of its secretion in the mammal. Advances Drug Res 11:23–74Google Scholar
  36. Tilders FJH, Post M, Jackson S, Lowry PJ (1980) Dual adrenergic control of the release of ACTH and other peptides from the rat intermediate lobe. Fed Proc 39:488Google Scholar
  37. Tilders FJH, Post M, Jackson S, Lowry PJ, Smelik PG (1981) Beta-adrenergic stimulation of the release of ACTH-and LPH-related peptides from the pars intermedia of the rat pituitary gland. Acta Endocrinol (Kbh) 97:343–351Google Scholar
  38. Van Wimersma Greidanus TB, Thody TJ, Verspaget H, de Rotte Ga, Goedemans HJH, Croiset G, Van Ree JM (1979) Effects of morphine and β-endorphin on basal and elevated plasma levels of α-MSH and vasopressin. Life Sci 24:579–586Google Scholar
  39. Vernaleone F, Devoto P, Marchisio AM, Stefanini E, Spano PF (1980) β-adrenergic sensitive adenylate cyclase in rat posterior pituitary. Pharmacol Res Comm 12:359–363Google Scholar
  40. Vinson GP, Whitehouse BJ, Dell A, Etienne T, Morris HR (1980) Characterisation of an adrenal zona glomerulosa-stimulating component of posterior pituitary extracts as α-MSH. Nature 284:464–467Google Scholar
  41. Weibel ER (1979) Stereological methods. Practical methods for biological morphometry. Academic Press, LondonGoogle Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • G. Schmitt
    • 1
  • M. E. Stoeckel
    • 1
  • M. J. Klein
    • 1
  • A. Porte
    • 1
  1. 1.Laboratoire de Physiologie Générale ULP LA 309 C.N.R.S.StrasbourgFrance

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