Advertisement

Applied Microbiology and Biotechnology

, Volume 43, Issue 3, pp 545–550 | Cite as

Glyphosate-degrading isolates from environmental samples: occurrence and pathways of degradation

  • R. E. Dick
  • J. P. Quinn
Environmental Biotechnology Original Paper

Abstract

The metabolism of the organophosphonate herbicide glyphosate was investigated in 163 environmental bacterial strains, obtained by a variety of isolation strategies from sites with or without prior exposure to the compound. Isolates able to use glyphosate as sole phosphorus source were more common at a treated site, but much less abundant than those capable of using the glyphosate metabolite aminomethyl-phosphonic acid (AMPA). Nevertheless, all 26 strains found to metabolise the herbicide did so via an initial cleavage of its carbon-phosphorus bond so via an initial cosine; no evidence for its metabolism or co-metabolism to AMPA was obtained.

Keywords

Phosphorus Bacterial Strain Environmental Sample Glyphosate Prior Exposure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Balthazor TM, Hallas LE (1986) Glyphosate-degrading microorganisms from industrial activated sludge. Appl Environ Microbiol 51:432–434Google Scholar
  2. Collins VG, Willoughby LG (1962) The distribution of bacteria and fungal spores in Blelham Tarn with particular reference to an experimental overturn. Arch Microbiol 43:294–307Google Scholar
  3. Dick RE (1991) Microbial degradation of the herbicide glyphosate. PhD Thesis, The Queen's University of Belfast, Belfast, N. IrelandGoogle Scholar
  4. Hallas LE, Hahn EN, Korndorfer C (1988) Characterization of microbial traits associated with glyphosate degradation in industrial activated sludge. J Ind Microbiol 3:377–385Google Scholar
  5. Horvath RS (1972) Microbial co-metabolism and the degradation of organic compounds in nature. Bacteriol Rev 36:146–155Google Scholar
  6. Jacob GS, Garbow JR, Hallas LE, Kimak NE, Kishore GH, Schaeffer J (1988) Metabolism of glyphosate in Pseudomonas sp. strain LBr. Appl Env Microbiol 54:2953–2958Google Scholar
  7. Kishore GH, Jacob GS (1987) Degradation of glyphosate by Pseudomonas sp. PG2982 via a sarcosine intermediate. J Biol Chem 262:12164–12168Google Scholar
  8. Krieg NR (1981) Enrichment and isolation. In: Gerhardt P (ed) Manual of methods for general bacteriology. ASM, Washington, pp 112–142Google Scholar
  9. Lee K-S, Metcalf WW, Wanner BL (1992) Evidence for two phosphonate degradative pathwaysin Enterobacter aerogenes. J Bacteriol 174:2501–2510Google Scholar
  10. Lerbs W, Stock M, Parthier B (1990) Physiological aspects of glyphosate degradation in Alcaligenes spec. strain GL. Arch Microbiol 153:146–150Google Scholar
  11. Liu C-M, McLean PA, Sookdeo CC, Cannon FC (1991) Degradation of the herbicide glyphosate by members of the family Rhizobiacae. Appl Environ Microbiol 57:1799–1804Google Scholar
  12. Malik J, Barry G, Kishore G (1989) The herbicide glyphosate (minireview). Biofactors 2:17–25Google Scholar
  13. McAuliffe KS, Hallas LE, Kulpa CF (1990) Glyphosate degradation by Agrobacterium radiobacter isolated from activated sludge. J Ind Microbiol 6:219–221Google Scholar
  14. Murthy DVS, Irvine RL, Hallas LE (1989) Principles of organism selection for the degradation of glyphosate in a sequencing batch reactor. In: Bell JM (ed) 43rd Annual Purdue Industrial Waste Conference. Lewis, Chelsea, MichGoogle Scholar
  15. Pipke R, Amrhein N (1988) Degradation of the phosphonate herbicide glyphosate by Arthrobacter atrocyaneus ATCC 13752. Appl Environ Microbiol 54:1293–1296Google Scholar
  16. Pipke R, Amrhein N, Jacob GS, Schaeffer J, Kishore GM (1987) Metabolism of glyphosate in an Arthrobacter sp. GLP-1. Eur J Biochem 165:267–273Google Scholar
  17. Quinn JP, Peden JMM, Dick RE (1988) Glyphosate tolerance and utilization by the microflora of soils treated with the herbicide. Appl Microbiol Biotechnol 29:511–516Google Scholar
  18. Quinn JP, Peden JMM, Dick RE (1989) Carbon-phosphorus bond cleavage by gram-positive and gram-negative soil bacteria. Appl Microbiol Biotechnol 31:283–287Google Scholar
  19. Rueppel ML, Brightwell BB, Schaeffer J, Marvel JT (1977) Metabolism and degradation of glyphosate in soil and water. J Agric Food Chem 25:517–528Google Scholar
  20. Shinabarger DL, Braymer HD (1986) Glyphosate catabolism by Pseudomonas sp. strain PG2982. J Bacteriol 168:702–707Google Scholar
  21. Sprankle P, Meggitt WF, Penner D (1975) Adsorption, mobility, and microbial degradation of glyphosate in the soil. Weed Sci 23:229–234Google Scholar
  22. Torstensson L (1985) Behavior of glyphosate in soils and its degradation. In: Grossbard E, Atkinson D (eds) The herbicide glyphosate. Butterworth, London, pp 137–150Google Scholar
  23. Verveij A, Boter HL, Dagenhardt CEAM (1979) Chemical warfare agents: verification of compounds containing the phosphorus-methyl linkage in waste water. Science 204:616–618Google Scholar
  24. Wanner BL, Metcalf WW (1992) Molecular genetic studies of a 10.9-kbp operon in Escherichia coli for phosphonate uptake and biodegradation. FEMS Microbiol Lett 100:133–140Google Scholar
  25. Weidhase R, Albrecht B, Stock M, Weidhase RA (1990) Glyphosatverwertung durch Pseudomonas sp. GS. Zentralbl Mikrobiol 145:433–438Google Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • R. E. Dick
    • 1
  • J. P. Quinn
    • 1
  1. 1.Division of Molecular Biology, School of Biology and BiochemistryThe Queen's University of BelfastBelfastN. Ireland

Personalised recommendations