Cell and Tissue Research

, Volume 247, Issue 2, pp 241–247 | Cite as

Rapid axoplasmic transport of insulin-like growth factor I in the sciatic nerve of adult rats

  • H. -A. Hansson
  • B. Rozell
  • A. Skottner
Article

Summary

Somatomedin C (Sm-C; insulin-like growth factor I; IGF-I) is a polypeptide (Mr 7649), often dependent on growth hormone (GH), with trophic effects on several different tissues. Monospecific IGF-I antisera were used to investigate its localization in the sciatic nerve and corresponding nerve cells, as well as its possible axoplasmic transport in the adult rat. IGF-I-like immunoreactivity was demonstrated in anterior horn motor nerve cells in the spinal cord and in spinal- and autonomic ganglion nerve cells. Faint IGF-I immunoreactivity was under normal conditions observed in axons of the sciatic nerve and in the Schwann cells. Using crush technique, accumulation of IGF-I immunoreactivity was seen in dilated axons within 2 h, both proximal and distal to the crush. However, only a small fraction of the anterogradely transported IGF-I immunoreactive material could be demonstrated to be transported in retrograde direction. Colchicine injected proximal to a crush prevented accumulation of IGF-I immunoreactivity proximal to the crush, but not distal to it.

IGF-I-immunoreactive material is synthesized in the cell bodies of peripheral sensory and motor nerve cells. It is transported at rapid rates in the axoplasm of the sciatic nerve of adult rats both in anterograde and retrograde directions. We propose that axonally transported IGF-I may be released and exert trophic influence on innervated cells, tissues and organs.

Key words

Somatomedin C Insulin-like growth factor I (IGF-I) Axoplasmic transport Sciatic nerve Schwann cell Trophic influence Rat 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Andersson I, Billig H, Fryklund L, Hansson H-A, Isaksson O, Isgaard J, Nilsson A, Rozell B, Skottner A, Stemme S (1986) Localization of IGF-I in adult rats. Immunohistochemical studies. Acta Physiol Scand 126:311–312Google Scholar
  2. Enberg G, Holmgren A (1985) Degradation of somatomedins by the thioredoxin system. FEBS Lett 183:52–54Google Scholar
  3. Furlanetto RW, Underwood LE, Van Wyk IJ, D'Ercole AJ (1977) Estimation of somatomedin-C levels in normals and patients with pituitary disease by radioimmunoassay. J Clin Invest 60:648–654Google Scholar
  4. Hansson HA, Dahlin LB, Danielsen N, Fryklund L, Nachemson AK, Polleryd P, Rozell B, Skottner A, Stemme S, Lundborg G (1986a) Evidence indicating trophic importance of IGF-I in regenerating peripheral nerve. Acta Physiol Scand 126:609–614Google Scholar
  5. Hansson HA, Petruson B, Skottner A (1986b) Somatomedin C in pathogenesis of malignant exophthalmos of endocrine origin. Lancet 25:218–219Google Scholar
  6. Haselbacher GK, Schwab ME, Pasi M, Humbel RE (1985) Insulin-like growth factor II (IGF-II) in the human brain: regional distribution of IGF II and of higher molecular mass forms. Proc Natl Acad Sci USA 82:2153–2157Google Scholar
  7. Hawkes R, Niday E, Gordon J (1982) A dot-immunobinding assay for monoclonal and other antibodies. Anal Biochem 119:142–147Google Scholar
  8. Jacobs S, Kull FC, Earp HS, Svoboda ME, Van Wyk JJ, Cuatrecasas P (1983) Somatomedin-C stimulates the phosphorylation of the b-subunit of its own receptor. J Biol Chem 258:9581–9584Google Scholar
  9. Karlsson JO (1982) Axonal transport of macromolecules. In: Brown IR (ed) Molecular Approaches to Neurobiology, Academic Press, New York, pp 131–157Google Scholar
  10. Kasuga M, Van Obberghen E, Nissley SP, Rechler MM (1981) Demonstration of two subtypes of insulin-like growth factor receptors by affinity cross-linking. J Biol Chem 256:5305–5308Google Scholar
  11. Kreutzberg GW (1969) Neuronal dynamics and axonal flow IV. Blockage of intra-axonal enzyme transport by colchicine. Proc Natl Acad Sci USA 62:722–728Google Scholar
  12. Laemmli UK (1979) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 277:680–685Google Scholar
  13. Lubinska L (1975) On axoplasmic flow. Int Res Neurobiol 17:241–296Google Scholar
  14. Ochs S (1982) Axoplasmic transport and its relation to other nerve functions. John Wiley & Sons, Inc, New YorkGoogle Scholar
  15. Rechler MM, Nissley SP (1985) The nature and regulation of the receptors for insulin-like growth factors. Ann Rev Physiol 47:425–442Google Scholar
  16. Rinderknecht E, Humbel RE (1978) The amino acid sequence of human insulin-like growth factor I and its structural homology with proinsulin. J Biol Chem 253:2769–2776Google Scholar
  17. Rozell B, Hansson HA, Luthman M, Holmgren A (1985) Immunohistochemical localization of thioredoxin and thioredoxin reductase in adult rats. Eur J Cell Biol 38:79–86Google Scholar
  18. Snyder EY, Kim SU (1980) Insulin: is it a nerve survival factor? Brain Res 196:565–571Google Scholar
  19. Stemme S, Hansson HA, Holmgren A, Rozell B (1985) Axoplasmic transport of thioredoxin and thioredoxin reductase in rat sciatic nerve. Brain Res 359:140–146Google Scholar
  20. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354PubMedGoogle Scholar
  21. Zapf J, Froesch ER, Humbel RE (1981) The insulin-like growth factors (IGF) of human serum: chemical and biological characterization and aspects of their possible physiological role. Curr Top Cell Regul 19:257–309Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • H. -A. Hansson
    • 1
  • B. Rozell
    • 1
  • A. Skottner
    • 2
  1. 1.Institute of NeurobiologyUniversity of GothenburgGothenburgSweden
  2. 2.KabiVitrum ABStockholmSweden

Personalised recommendations