Cell and Tissue Research

, Volume 243, Issue 3, pp 579–585

Relation of actin fibrils to energy metabolism of endothelial cells

  • Utz Tillmann
  • Jürgen Bereiter-Hahn


The physiological significance of the association of glycolytic enzymes with actin fibrils was investigated in cell culture. Cytochalasin D (CD) was used to induce the known actin-based sequence of events in a culture of an endothelial-cell line (XTH-2) derived from hearts from tadpoles of Xenopus laevis. 1 min following addition of CD, ruptures in the cortical fibrillar meshwork and in stress fibres are seen. At the same time the cellular ATP level decreases by ca. 25%. This and the following reactions resulting in a kind of arborization depend on a continuous supply with metabolic energy. As shown by measurements of oxygen consumption, cells with intact energy metabolism provide the ATP needed from glycolysis; ATP produced by oxidative phosphorylation is not ultilized as long as lactate dehydrogenase (LDH) reoxidizes NADH2. After inhibition of LDH, respiration in XTH-2 cells doubles. CD treatment induces a transient increase in oxygen consumption, indicating an increased energy supply by respiration. From these results we conclude: The energy needed by the actomyosin system is — under normal metabolic conditions — supplied from ATP phosphorylated in glycolysis. The processes of energy metabolism seem to be highly compartmentalized; ATP is not a parameter that is kept constant in time intervals of minutes up to one hour.

Key words

Actin Glycolysis Respiration Cellular ATP-content Cytolchalasin D Endothelial cells Cell culture Xenopus laevis 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bereiter-Hahn J, Tillmann U, Vöth M (1984) Interaction of metabolic inhibitors with actin fibrils. Cell Tissue Res 238:129–134Google Scholar
  2. Brändle K (1984) Nichtlineare Kurvenanpassung für die Serie HP 80 von Hewlett-Packard. Firma Coralis Team, FriedrichsdorfGoogle Scholar
  3. Clarke F, Stephan P, Morton D, Weidemann J (1983) The role of actin and associated proteins in the organisation of glycolytic enzymes. In: Dos Remedios CG, Barden JA (eds) Actin. Academic Press, Sydney New York, pp 249–258Google Scholar
  4. Colowick SP, Nagarajan B (1972) Energy sources for tumor growth. In: San Pietro A, Gest H (eds) Horizons of Bioenergetics. Academic Press, New York London pp 97–112Google Scholar
  5. Dancker P, Kliche A (1981) Cytochalasin B-induced ATPase activity of actin: Dependence on monomer concentration. Z Naturforsch 36c:1050–1055Google Scholar
  6. Ester EE, Selden LA, Gershman LC (1983) The exchange of nucleotide bound to monomer actin limits the rate of cytochalasininduced ATP hydrolysis. In: Dos Remedios CG, Barden JA (eds) Actin. Academic Press, Syndney New York, pp 169–176Google Scholar
  7. Faulstich H, Trischmann H, Mayer D (1983) Preparation of tetramethyl-rhodaminyl-phalloidin and uptake of the toxin into short-term cultured hepatocytes by endocytosis. Exp Cell Res 144:73–82Google Scholar
  8. Furcht CT, Wendelschafer-Crabb G (1978) Trypsin-induced coordinate alterations in cell shape, cytoskeleton, and intrinsic membrane structure of contact-inhibited cells. Exp Cell Res 144:1–14Google Scholar
  9. Godman G, Woda B, Kolberg R (1980a) Redistribution of contractile and cytoskeletal components induced by cytochalasin. I. In Hmf cells, a nontransformed fibroblastoid line. Eur J Cell Biol 22:733–744Google Scholar
  10. Godman G, Woda B, Kolberg R (1980b) Redistribution of contractile and cytoskeletal components induced by cytochalasin. II. In HeLa and HEp2 cells. Eur J Cell Biol 22:745–754Google Scholar
  11. Heacock CS, Eidsvoog KE, Bamburg JR (1984) The influence of contact-inhibited growth and of agents which alter cell morphology on the levels of Gand F-actin in cultured cells. Exp Cell Res 153:402–412Google Scholar
  12. Kajstura J, Korohoda W (1983) Significance of energy metabolism pathways for stimulation of DNA synthesis by cell migration and serum. Eur J Cell Biol 31:9–14Google Scholar
  13. Korohoda W, Kalisz B (1970) Correlation of respiratory and motile activities in Amoeba proteus. Folia Biol 18:137–143Google Scholar
  14. Korohoda W, Shraideh Z, Baranowski Z, Wohlfarth-Bottermann KE (1983) Energy metabolic regulation of oscillatory contraction activity in Physarum polycephalum. Cell Tissue Res 231:675–691Google Scholar
  15. Masters C (1984) Interactions between glycolytic enzymes and components of the cytomatrix. J Cell Biol 99:222s-225sGoogle Scholar
  16. Masters CJ, Wilson JE (1981) Interactions between soluble enzymes and subcellular structure. CRC Crit Rev Biochem II, 105–141Google Scholar
  17. McKeehan WL (1982) Glycolysis, glutaminolysis and cell proliferation. Cell Biol Int Rep 6:635–650Google Scholar
  18. Morris A, Tannenbaum J (1980) Cytochalasin D does not produce net depolymerization of actin filaments in HEp-2 cells. Nature 287:637Google Scholar
  19. Rose GG, Pomerat CM, Shindler TO, Trunnell JB (1958) A cellophane-strip technique for culturing tissue in multipurpose culture chambers. J Biophys Biochem Cytol 4:761–764Google Scholar
  20. Sanger JW, Sanger JM, Jockusch BM (1983) Differential response of three types of actin filament bundles to depletion of cellular ATP levels. Eur J Cell Biol 31:197–204Google Scholar
  21. Schlage W, Bereiter-Hahn J (1981) Established Xenopus tadpole heart endothelium (XTH) cells exhibiting selected properties of primary cells. Eur J Cell Biol 24:342Google Scholar
  22. Schlage WK, Bereiter-Hahn J (1983) A microscope perfusion respirometer for continuous respiration measurement of cultured cells during microscopic observation. Microscopica Acta 87:19–34Google Scholar
  23. Schliwa M (1982) Action of cytochalasin D on cytoskeletal networks J Cell Biol 92:79–91Google Scholar
  24. Wegner A (1977) The mechanism of ATP hydrolysis by polymer actin. Biophys Chem 7:51–58Google Scholar
  25. Wolf K, Quimby MC (1962) Synthetic media. Amphibian culture medium (Wolf & Quimby). Excerpta Medica 16: No. 9Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Utz Tillmann
    • 1
  • Jürgen Bereiter-Hahn
    • 1
  1. 1.Cinematic Cell Research GroupJ.W. Goethe UniversityFrankfurt a.M.Federal Republic of Germany
  2. 2.Arbeitsgruppe Kinematische Zellforschung, Fachbereich BiologieFrankfurt am MainFederal Republic of Germany

Personalised recommendations