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Cell and Tissue Research

, Volume 243, Issue 3, pp 545–559 | Cite as

Light and electron-microscopic immunocytochemistry and lectin histochemistry of the subcommissural organ: Evidence for processing of the secretory material

  • Estéban M. Rodríguez
  • Hernán Herrera
  • Bruno Peruzzo
  • Sara Rodríguez
  • Silvia Hein
  • Andreas Oksche
Article

Summary

The subcommissural organ (SCO) of the rat was investigated by use of histochemical and immunocytochemical methods at the light and electron-microscopic levels. Consecutive thin methacrylate sections were stained with the pseudoisocyanin (Psi), immunoperoxidase (IMC; employing an antiserum against Reissner's fiber, AFRU), periodic acid-Schiff (PAS) and periodic acid-silver methenamine (SM) techniques, and reacted with six types of lectins. Psi, SM, concanavalin A (Con A) and IMC were also used for double and triple sequential staining of the same section. Increasing dilutions of AFRU (from 1∶1000 to 1∶200 000) were used for immunostaining of serial paraffin sections. In addition, ultrastructural localization of (i) Con A-binding sites and (ii) immunoreactive secretory material was performed. Some of these procedures were also applied to the ophidian and canine SCO.

Con A-positive, Psi-positive and immunoreactive materials coexisted within the same cisternae of the rough endoplasmic reticulum. The Golgi apparatus lacked Con A-positive and immunoreactive substances. Apical secretory granules and secreted material lying on the surface of the SCO showed (i) the highest affinity for AFRU, but were (ii) Con A-negative, and (iii) wheat-germ agglutinin-, PAS and SM-positive. Reissner's fiber displayed a low affinity for AFRU.

It is suggested that the SCO secretes N-linked glycoproteins, the carbohydrate and protein moeities of which undergo (i) a maturation process before being released, and (ii) some kind of modification(s) after their release into the ventricle. The perivascular secretory cells of the dog SCO might secrete a material different from that secreted by the ependymal cells.

Key words

Subcommissural organ Glycoproteins Secretory process Immunocytochemistry Lectin histochemistry Rat Vertebrates 

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Estéban M. Rodríguez
    • 1
    • 2
  • Hernán Herrera
    • 1
    • 2
  • Bruno Peruzzo
    • 1
    • 2
  • Sara Rodríguez
    • 1
    • 2
  • Silvia Hein
    • 1
    • 2
  • Andreas Oksche
    • 1
    • 2
  1. 1.Instituto de Histología y Patología, Universidad Austral de ChileValdiviaChile
  2. 2.Department of Anatomy and CytobiologyJustus Liebig University of GiessenGiessenFederal Republic of Germany

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