Human Genetics

, Volume 91, Issue 4, pp 403–404 | Cite as

Polymorphism in human IL-1 receptor antagonist gene intron 2 is caused by variable numbers of an 86-bp tandem repeat

  • Joanna K. Tarlow
  • Alexandra I. F. Blakemore
  • Andrew Lennard
  • Roberto Solari
  • Howard N. Hughes
  • Alexander Steinkasserer
  • Gordon W. Duff
DNA Variants

Abstract

We have investigated the polymorphism in intron 2 of the interleukin-1 receptor antagonist gene and identified two new alleles of the system. We have shown that the polymorphism is caused by the variable copy number of an 86-bp sequence, by using the polymerase chain reaction and primers immediately flanking the repeat region, and by direct sequencing. The repeat region contains three potential protein-binding sites and therefore the variable copy number may have functional significance.

Keywords

Polymerase Chain Reaction Tandem Repeat Direct Sequencing Functional Significance Variable Copy Number 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Joanna K. Tarlow
    • 1
  • Alexandra I. F. Blakemore
    • 1
  • Andrew Lennard
    • 2
  • Roberto Solari
    • 2
  • Howard N. Hughes
    • 3
  • Alexander Steinkasserer
    • 4
  • Gordon W. Duff
    • 1
  1. 1.Section of Molecular Medicine, Department of MedicineUniversity of Sheffield, Royal Hallamshire HospitalSheffieldUK
  2. 2.Yamanouchi Research Institute (UK)OxfordUK
  3. 3.Department of Biological SciencesThe Manchester Metropolitan UniversityManchesterUK
  4. 4.MRC Immunochemistry Unit, Department of BiochemistryUniversity of OxfordOxfordUK

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