Theoretical and Applied Genetics

, Volume 81, Issue 2, pp 157–161 | Cite as

Large-scale deletions of rice plastid DNA in anther culture

  • T. Harada
  • T. Sato
  • D. Asaka
  • I. Matsukawa


Plastid DNA (ptDNA) in albino rice plants regenerated from pollen by anther culture was investigated by Southern blotting. Of the 20 albino plants investigated, 7 contained ptDNA that had suffered large-scale deletion. The size and location of the deletions differed among the plants. In all cases about 30 kbp of the region containing the PstI-2 fragment (15.7 kbp) had been retained. The deleted ptDNA molecules were retained in calluses derived from the roots of each albino plant.

Key words

Plastid DNA Anther culture Rice Albino Pollen 


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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • T. Harada
    • 1
  • T. Sato
    • 1
  • D. Asaka
    • 1
  • I. Matsukawa
    • 1
  1. 1.Hokkaido Central Agricultural Experiment StationHokkaidoJapan

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