Cell and Tissue Research

, Volume 251, Issue 1, pp 137–143 | Cite as

Localization of CRF-immunoreactive neurons in the cat medulla oblongata: their presence in the inferior olive

  • Kunio Kitahama
  • Pierre-Hervé Luppi
  • Gérard Tramu
  • Jean-Pierre Sastre
  • Colette Buda
  • Michel Jouvet


Corticotropin releasing factor (CRF)-immunoreactive (IR) perikarya, visualized by the indirect immunoperoxidase method in colchicine-pretreated cats, were localized in many discrete regions of the medulla oblongata. They were found mainly in the dorsal aspect and midline of the medulla oblongata, and more rostrally in the ventrolateral portion. Our results also demonstrated CRF-IR neurons in the rostrocaudal extent of the inferior olive, probably projecting to the cerebellar cortex via thick axons visualized along the lateral edge of the medulla. CRF-IR olivary cells were also found in the pontine cat from which the forebrain was removed, but neither in hypophysectomized nor adrenalectomized cats.

Key words

Corticotropin-releasing factor (CRF) Medulla oblongata Inferior olive Immunohistochemistry Cat 


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  1. Angaut P, Alvarade-Mallart RM, Sotelo C (1985) Compensatory climbing fiber innervation after unilateral pedunculotomy in the newborn rat: origin and topographic organization. J Comp Neurol 236:161–178PubMedGoogle Scholar
  2. Berman AL (1968) The Brain Stem of the Cat. A Cytoarchitectonic Atlas With stereotaxic Coordinates. University of Wisconsin Press, Madison, Milwaukee and LondonGoogle Scholar
  3. Bleier R, Bard P, Woods JW (1966) Cytoarchitectonic appearance of the isolated hypothalamus of the cat. J Comp Neurol 128:255–312PubMedGoogle Scholar
  4. Bloom FE, Battenberg ELF, Rivier J, Vale W (1982) Corticotropin-releasing factor (CRF)-immunoreactive neurones and fibers in rat hypothalamus. Regul Pept 4:43–48PubMedGoogle Scholar
  5. Boesten AJP, Voogd J (1985) Hypertrophy of neurons in the inferior olive after cerebellar ablations in the cat. Neurosci Lett 61:49–54PubMedGoogle Scholar
  6. Bugnon C, Fellmann D, Gouget A, Cardot J (1982) Mise en évidence immunocytochimique du système neuroglandulaire à CRF dans l'hypothalamus du rat. C R Acad Sci 294:279–284Google Scholar
  7. Cummings S, Elde R, Ells J, Lindall A (1983) Corticotropin-releasing factor immunoreactivity is widely distributed within the central nervous system of the rat: an immunohistochemical study. J Neurosci 3:1355–1368PubMedGoogle Scholar
  8. Cummings S, Elde R, Sharp B (1985) CRF-immunoreactive neurons within the inferior olive project to the flocculus and dorsal and ventral paraflocculi. 15th Annual meeting Society for Neuroscience abstract, 11:204.18Google Scholar
  9. Desclin JC (1974) Histological evidence supporting the inferior olive as the major source of cerebellar climbing fibers in the rat. Brain Res 77:365–384PubMedGoogle Scholar
  10. Fellmann D, Bugnon C, Bresson JL, Gouget A, Cardot J, Clavequin MC, Hadjiyiassemis M (1984) The CRF neuron: immunocytochemical study. Peptides 5:19–33PubMedGoogle Scholar
  11. Hancock MB (1982) A serotonin-immunoreactive fiber system in the dorsal columns of the spinal cord. Neurosci Lett 21:247–252Google Scholar
  12. Jouvet M (1965) Etude de la dualité des états de sommeil et des mécanismes de la phase paradoxale. In Jouvet M (ed) Aspects anatomo-fonctionnels de la physiologie du sommeil. CNRS 397–449Google Scholar
  13. Jouvet M, Sastre JP, Buda C, Janin M, Kitahama K, Sallanon M (1984) La pars intermédia de l'hypophyse est-elle responsable du déclenchement du sommeil paradoxal chez le Chat pontique avec “hypophyse isolée”? C R Acad Sci 299:883–888Google Scholar
  14. Joseph SA, Knigge KM (1983) Corticotropin releasing factor: immunocytochemical localization in rat brain. Neurosci Lett 35:135–141PubMedGoogle Scholar
  15. Kawamura K, Hashikawa T (1979) Olivocerebellar projections in the cat studied by means of anterograde axonal transport of labeled amino acids as tracers. Neuroscience 4:1625–1633Google Scholar
  16. Kitahama K, Pearson J, Denoroy L, Kopp N, Ulrich J, Maeda T, Jouvet M (1985) Adrenergic neurons in human brain demonstrated by immunohistochemistry with antibodies to phenylethanolamine-N-methyltransferase (PNMT): Discovery of a new group in the nucleus tractus solitarius. Neurosci Lett 53:303–308PubMedGoogle Scholar
  17. Kolodziejczyk E, Baertschi AJ, Tramu G (1983) Corticoliberinimmunoreactive cell bodies localized in two distinct areas of the sheep hypothalamus. Neuroscience 9:261–270PubMedGoogle Scholar
  18. Merchenthaler I (1984) Corticotropin releasing factor (CRF)-like immunoreactivity in the rat central nervous system. Extrahypothalamic distribution. Peptides 5:53–69Google Scholar
  19. Ogawa T (1939) The tractus tegmenti medialis and its connection with the inferior olive in the cat. J Comp Neurol 70:181–190Google Scholar
  20. Olschowka JA, O'Donohue TL, Mueller GP, Jacobowitz DM (1982) The distribution of corticotropin releasing factor-like immunoreactive neurons in rat brain. Peptides 3:995–1015PubMedGoogle Scholar
  21. Palkovits M, Brownstein MJ, Vale W (1985) Distribution of corticotropin-releasing factor in rat brain. Federation Proc 44:215–219Google Scholar
  22. Paull WK, Phelix CF, Copeland M, Palmiter P, Gibbs FP, Middleton C (1984) Immunohistochemical localization of corticotropin releasing factor (CRF) in the hypothalamus of the squirrel monkey, Saimiri sciureus. Peptides 5:45–51PubMedGoogle Scholar
  23. Pelletier G, Désy L, Côté J, Vaudry H (1983) Immunocytochemical localization of corticotropin-releasing factor-like immunoreactivity in the human hypothalamus. Neurosci Lett 41:259–263PubMedGoogle Scholar
  24. Pilcher WH, Joseph SA (1984) Co-localization of CRF-ir perikarya and ACTH-ir fibers in rat brain. Brain Res 299:91–102PubMedGoogle Scholar
  25. Rémy C, Tramu G, Dubois MP (1982) Immunohistological demonstration of a CRF-like material in the central nervous system of the annelid Dendrobaena, Cell Tissue Res 227:569–575PubMedGoogle Scholar
  26. Saint Cyr JA, Courville J (1982) Descending projections to the inferior olive from the mesencephalon and superior colliculus in the cat. An autoradiographic study. Exp Brain Res 45:333–348PubMedGoogle Scholar
  27. Schipper J, Steinbusch HWM, Vermes I, Tilders FJH (1983) Mapping of CRF-immunoreactive nerve fibers in the medulla oblongata and spinal cord of the rat. Brain Res 267:145–150PubMedGoogle Scholar
  28. Skofitsch G, Jacobowitz DM (1985) Distribution of corticotropin releasing factor-like immunoreactivity in the rat brain by immunohistochemistry and radioimmunoassay: comparison and characterization of ovine and rat/human CRF antisera. Peptides 6:319–336PubMedGoogle Scholar
  29. Swanson LW, Sawchenko PE, Rivier J, Vale WW (1983) Organization of ovine corticotropin-releasing factor immunoreactive cells and fibers in the rat brain; an immunohistochemical study. Neuroendocrinology 36:165–186PubMedGoogle Scholar
  30. Taber E (1961) The cytoarchitecture of the brain stem of the cat. I. Brain stem nuclei of the cat. J Comp neurol 116:27–69PubMedGoogle Scholar
  31. Tramu G, Pillez A (1982) Localisation immunohistochimique des terminaisons nerveuses à corticoliberine (CRF) dans l'éminence médiane du cobaye et du rat. C R Acad Sci 294:107–114Google Scholar
  32. Vale W, Spiess J, Rivier C, Rivier J (1981) Characterization of a 41-residue ovine hypothalamic peptide that stimulates secretion of corticotropin and β-endorphin. Science 213:1394–1397PubMedGoogle Scholar
  33. Ward DG, Gann DS (1976) Inhibitory and facilitatory areas of the dorsal medulla mediating ACTH release in the cat. Endocrinology 99:1213–1219PubMedGoogle Scholar
  34. Wynn PC, Hauger M, Holmes MC, Millan MA, Catt KJ, Aguilera G (1984) Brain and pituitary receptors for corticotropin releasing factor: localization and differential regulation after adrenalectomy. Peptides 5:1077–1084PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Kunio Kitahama
    • 1
  • Pierre-Hervé Luppi
    • 1
  • Gérard Tramu
    • 2
  • Jean-Pierre Sastre
    • 1
  • Colette Buda
    • 1
  • Michel Jouvet
    • 1
  1. 1.Département de Médecine ExpérimentaleINSERM U52, CNRS UA1195, Faculté de MédecineLyonFrance
  2. 2.INSERM U156, Place de VerdunLilleFrance

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