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Cell and Tissue Research

, Volume 251, Issue 1, pp 137–143 | Cite as

Localization of CRF-immunoreactive neurons in the cat medulla oblongata: their presence in the inferior olive

  • Kunio Kitahama
  • Pierre-Hervé Luppi
  • Gérard Tramu
  • Jean-Pierre Sastre
  • Colette Buda
  • Michel Jouvet
Article

Summary

Corticotropin releasing factor (CRF)-immunoreactive (IR) perikarya, visualized by the indirect immunoperoxidase method in colchicine-pretreated cats, were localized in many discrete regions of the medulla oblongata. They were found mainly in the dorsal aspect and midline of the medulla oblongata, and more rostrally in the ventrolateral portion. Our results also demonstrated CRF-IR neurons in the rostrocaudal extent of the inferior olive, probably projecting to the cerebellar cortex via thick axons visualized along the lateral edge of the medulla. CRF-IR olivary cells were also found in the pontine cat from which the forebrain was removed, but neither in hypophysectomized nor adrenalectomized cats.

Key words

Corticotropin-releasing factor (CRF) Medulla oblongata Inferior olive Immunohistochemistry Cat 

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Kunio Kitahama
    • 1
  • Pierre-Hervé Luppi
    • 1
  • Gérard Tramu
    • 2
  • Jean-Pierre Sastre
    • 1
  • Colette Buda
    • 1
  • Michel Jouvet
    • 1
  1. 1.Département de Médecine ExpérimentaleINSERM U52, CNRS UA1195, Faculté de MédecineLyonFrance
  2. 2.INSERM U156, Place de VerdunLilleFrance

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