Advertisement

Cell and Tissue Research

, Volume 251, Issue 1, pp 95–103 | Cite as

Ultrastructure of the aesthetasc (olfactory) sensilla of the spiny lobster, Panulirus argus

  • Ulrike Grünert
  • Barry W. Ache
Article

Summary

The aesthetascs of the spiny lobster, Panulirus argus, are hair sensilla located on the lateral filaments of the antennules. Each hair is about 0.8 mm long and innervated by about 320 bipolar sensory neurons, the dendrites of which project as a bundle into the hair shaft. Each of the dendrites develops two cilia. Within a very short distance each of these cilia branches repetitively and dichotomously resulting in 8000 to 10000 outer dendritic segments per hair, or about 20 to 30 branches per neuron. The branches intertwine frequently before running to the tip of the hair. Each hair also possesses inner and outer auxiliary cells. The inner auxiliary cells surround the bundle of dendrites, extending distally to the origin of the ciliary segments. Extensions of these cells project into the bundle of dendrites, separating groups of dendrites into discrete clusters. Outer auxiliary cells wrap the inner ones, but do not extend beyond the base of the hair.

Key words

Chemoreception Olfaction Sensilla Spiny lobster (Panulirus argus

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ache BW, Derby CD (1985) Functional organization of olfaction in crustaceans. TINS 8:356–360Google Scholar
  2. Altner H, Prillinger L (1980) Ultrastructure of invertebrate chemo-, thermo-, and hygroreceptors and its functional significance. Int Rev Cytol 67:69–139Google Scholar
  3. Altner I, Hatt H, Altner H (1983) Structural properties of bimodal chemoand mechanosensitive setae on the periopod chelae of the crayfish, Austropotamobius torrentium. Cell Tissue Res 228:357–374PubMedGoogle Scholar
  4. Altner H, Hatt H, Altner I (1986) Structural and functional properties of the mechanoreceptors and chemoreceptors in the anterior oesophageal sensilla of the crayfish, Astacus astacus. Cell Tissue Res 244:537–547Google Scholar
  5. Anderson PAV, Ache BW (1985) Voltageand current clamp recordings of the receptor potential in olfactory cells in situ. Brain Res 338:273–280PubMedGoogle Scholar
  6. Atema J (1977) Functional separation of smell and taste in fish and Crustacea. In: Le Magnen J, MacLeod (eds) Olfaction and Taste VI, Information Retrieval, London Washington, pp 165–174Google Scholar
  7. Bauchau AG, Passelecq-Gérin E (1984) Ultrastructure des aesthetascs du crabe Carcinus maenas, L., Cah Biol Mar 25:333–341Google Scholar
  8. Burton PR (1985) Ultrastructure of the olfactory neuron of the bullfrog: the dendrite and its microtubules. J Comp Neurol 242:147–160PubMedGoogle Scholar
  9. Carr WES, Ache BW, Gleeson RA (1987) Chemoreceptors of crustaceans: similarities to receptors for neuroactive substances in internal tissues. Environ Health Perspect 71:31–46PubMedGoogle Scholar
  10. Crouau Y (1978) Organes sensoriels d'un mysidacé souterrain anophthalme, Antromysis juberthiei: étude ultrastructurale des aesthetascs. Bull Mus Natn Hist Nat Paris 513:165–175Google Scholar
  11. Derby CD, Atema J (1987) Chemoreceptor cells in aquatic invertebrates: peripheral mechanisms of chemical signal processing in decapod crustaceans. In: Atema J, Fay RR, Popper AN, Tavolga WN (eds) Sensory biology of aquatic animals. Springer, Berlin Heidelberg New York (in press)Google Scholar
  12. Dustin P (1978) Microtubules. Springer, Berlin Heidelberg New YorkGoogle Scholar
  13. Ernst K.-D (1972) Die Ontogenie der basiconischen Riechsensillen auf der Antenne von Necrophorus (Coleoptera). Z Zellforsch 129:217–236PubMedGoogle Scholar
  14. Frisch D (1969) Notes on the fine structure of olfactory epithelium. In: Pfaffmann C (ed) Olfaction and Taste III. Rockefeller University Press, New York, pp 22–33Google Scholar
  15. Frisch D, Everingham JW (1972) Fine structure of crab olfactory cilia: non-chemical fixation; environmental effects. In: Schneider D (ed) Olfaction and Taste IV. Wissenschaftliche Verlagsgesellschaft, Stuttgart, pp 5–12Google Scholar
  16. Ghiradella H, Case J, Cronshaw J (1968a) Fine structure of the aesthetasc hairs of Coenobita compressus Edwards. J Morphol 124:361–386PubMedGoogle Scholar
  17. Ghiradella H, Case J, Cronshaw J (1968b) Structure of aesthetascs in selected marine and terrestrial decapods: chemoreceptor morphology and environment. Am Zool 8:603–621PubMedGoogle Scholar
  18. Ghiradella H, Cronshaw J, Case J (1968c) Fine structure of the aesthetasc hairs of Pagurus hirsutiusculus Dana. Protoplasma 66:1–20Google Scholar
  19. Gnatzy W, Schmidt M, Römbke J (1984) Are the funnel-canal organs the “campaniform sensilla” of the shore crab Carcinus maenas (Crustacea, Decapoda)? I. Topography, external structure and basic organization. Zoomorphology 104:11–20Google Scholar
  20. Graziadei PPC (1971) The olfactory mucosa of vertebrates. In: Beidler LM (ed) Handbook of sensory physiology. Vol 4 Chemical Senses I Olfaction. Springer, Berlin Heidelberg New York, pp 27–58Google Scholar
  21. Guse G-W (1983) Ultrastructure, development, and moulting of the aesthetascs of Neomysis integer and Idotea baltica (Crustacea, Malacostraca). Zoomorphology 103:121–133Google Scholar
  22. Heimann P (1984) Fine structure and molting of aesthetasc sense organs on the antennules of the isopod, Asellus aquaticus (Crustacea). Cell Tissue Res 235:117–128PubMedGoogle Scholar
  23. Juberthie-Jupeau L, Crouau Y (1977) Ultrastructure des aesthetascs d'un mysidacé souterrain anophthalme. CR Acad Se Paris Ser D 284:2257–2259Google Scholar
  24. Keil TA (1984a) Reconstruction and morphometry of silkmoth olfactory hairs: A comparative study of sensilla trichodea on the antennae of male Antheraea polyphemus and Antheraea pernyi (Insecta, Lepidoptera). Zoomorphology 104:142–156Google Scholar
  25. Keil TA (1984b) Surface coats of pore tubules and olfactory sensory dendrites of a silkmoth revealed by cationic markers. Tissue Cell 16:705–717PubMedGoogle Scholar
  26. Laverack MS, Ardill DJ (1965) The innervation of the aesthetasc hairs of Panulirus argus. Q J Microsc Sci 106:45–60Google Scholar
  27. Lidow MS, Menco BPM (1984) Observations on axonemes and membranes of olfactory and respiratory cilia in frogs and rats using tannic acid-supplemented fixation and photographic rotation. J Ultrastruct Res 86:18–30PubMedGoogle Scholar
  28. Menco BPM (1984) Ciliated and microvillous structures of rat olfactory and nasal respiratory epithelia. A study using ultrarapid cryofixation followed by freeze-substitution or freezeetching. Cell Tissue Res 235:225–241PubMedGoogle Scholar
  29. Olesco-Szuts S, Atema J (1977) Chemical senses of the lobster, Homarus americanus: fine structure of aesthetasc hairs. In: Phillips BF, Cobb JS (eds) Workshop on lobster and rock lobster ecology and physiology. Div Fish Ocean Circular 7, CSIRO, Melbourne, pp 16–17Google Scholar
  30. Reynolds ES (1963) The use of lead citrate at high pH as an electron opaque strain in electron microscopy. J Cell Biol 17:208–212PubMedGoogle Scholar
  31. Satir P (1977) Microvilli and cilia: surface specializations of mammalian cells. In: Jamieson GA, Robinson DM (eds) Mammalian cell membranes. 2. The diversity of membranes. Butterworths, Boston, pp 323–353Google Scholar
  32. Schmidt M, Gnatzy W (1984) Are the funnel-canal organs the ‘campaniform sensilla’ of the shore crab, Carcinus maenas (Decapoda, Crustacea)? II. Ultrastructure. Cell Tissue Res 237:81–93PubMedGoogle Scholar
  33. Schmidt M, Gnatzy W (1986) Contact chemoreceptors on the walking legs of the shore crab, Carcinus maenas. Chem Senses 11:657Google Scholar
  34. Schmiedel-Jakob I, Anderson PAV, Ache BW (1986) Intracellular recording of the receptor potential in primary chemosensory neurons. Chem Senses 11:657–658Google Scholar
  35. Snow PJ (1973) Ultrastructure of the aesthetasc hairs of the littoral decapod, Paragrapsus gaimardii. Z Zellforsch 138:489–502PubMedGoogle Scholar
  36. Spencer M (1986) The innervation and chemical sensitivity of single aesthetasc hairs. J Comp Physiol A 158:59–68Google Scholar
  37. Spencer M, Linberg KA (1986) Ultrastructure of aesthetasc innervation and external morphology of the lateral antennule setae of the spiny lobster Panulirus interruptus (Randall). Cell Tissue Res 245:69–80PubMedGoogle Scholar
  38. Tierney AJ, Thompson CS, Dunham DW (1986) Fine structure of aesthetasc chemoreceptors in the crayfish Orconectes propinquus. Can J Zool 64:392–399Google Scholar
  39. Trapido-Rosenthal HG, Carr WES, Gleeson RA (1987) Biochemistry of an olfactory purinergic system: Dephosphorylation of excitatory nucleotides and uptake of adenosine. J Neurochem (in press)Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Ulrike Grünert
    • 1
  • Barry W. Ache
    • 1
  1. 1.C.V. Whitney LaboratoryUniversity of FloridaSt. AugustineUSA

Personalised recommendations