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Cell and Tissue Research

, Volume 238, Issue 1, pp 113–119 | Cite as

Localization of a peptide identified by antibodies to gastrin/CCK in the gut of Cancer magister

  • Frederick W. Scalise
  • Brett A. Larson
  • Steven R. Vigna
Article

Summary

A gastric peptide from the Dungeness crab (Cancer magister), extracted and characterized previously (Larson and Vigna 1983b), was localized in the foregut (stomach) of this species by immunocytochemistry using antisera specific for the bioactive carboxy-terminal amino acid sequence common to gastrins and cholecystokinins (CCKs). Immunoreactivity was found in all gastric epithelial cells and in the procuticle. Electron microscopy revealed an absence of peptidergic secretory granules in the gastric epithelial cells. The pattern of immunostaining suggests that the gastric epithelial cells secrete this peptide apically where it is incorporated into the cuticle lining the lumen. Specific immunostaining could not be demonstrated in various neural ganglia or in the hypodermis. The distribution of this peptide is different from that of gastrin/CCK in vertebrates and other invertebrates. This suggests that the crab gastric peptide is sufficiently similar to gastrin/CCK to react with C-terminal specific antisera, but may be anatomically, functionally, and possibly phylogenetically otherwise unrelated.

Key words

Crustacea Gastrointestinal hormones Neuropeptides Immunocytochemistry Cuticle Cancer magister 

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References

  1. Barker PL, Gibson R (1977) Observations on the feeding mechanism, structure of the gut, and digestive physiology of the European lobster Homarus gammarus. J Exp Mar Biol Ecol 26:297–324Google Scholar
  2. Buchan AMJ, Polak JM, Solcia E, Pearse AGE (1979) Localization of intestinal gastrin in a distinct endocrine cell type. Nature 277:138–140Google Scholar
  3. Dockray GJ (1980) Cholecystokinin in rat cerebral cortex: identification, purification and characterization by immunochemical methods. Brain Res 188:155–166Google Scholar
  4. Dockray GJ (1982) The physiology of cholecystokinin in brain and gut. Br Med Bull 38:253–258Google Scholar
  5. Dockray GJ, Hopkins CR (1975) Caerulein secretion by dermal glands in Xenopus laevis. J Cell Biol 64:724–733Google Scholar
  6. Dockray GJ, Duve H, Thorpe A (1981a) Immunochemical characterization of gastrin/cholecystokinin-like peptides in the brain of the blowfly, Calliphora vomitoria. Gen Comp Endocrinol 45:491–496Google Scholar
  7. Dockray GJ, Williams RG, Zhu W-Y (1981b) Development of region-specific antisera for the C-terminal tetrapeptide of gastrin/cholecystokinin and their use in studies of immunoreactive forms of cholecystokinin in rat brain. Neurochem Int 3:281–288Google Scholar
  8. Factor JR (1981) Development and metamorphosis of the digestive system of larval lobsters, Homarus americanus. J Morphol 169:225–242Google Scholar
  9. Falkmer S, Carraway RE, El-Salhy M, Emdin SO, Grimelius L, Rehfeld JF, Reinecke M, Schwartz TW (1981) Phylogeny of the gastroenteropancreatic neuroendocrine system: A review. In: Grossman MI, Brazier MAB, Lechago J (eds) Cellular basis of chemical messengers in the digestive system. Academic Press, New York, p 21Google Scholar
  10. Fritsch HR, Van Noorden S, Pearse AGE (1978) Localization of somatostain- and gastrin-like immunoreactivity in the gastrointestinal tract of Ciona intestinalis. Cell Tissue Res 180:181–185Google Scholar
  11. Grimmelikhuijzen CJP, Sundler F, Rehfeld JF (1980) Gastrin/ CCK-like immunoreactivity in the nervous system of coelenterates. Histochemistry 69:61–68PubMedGoogle Scholar
  12. Hackman RH (1971) The integument of Arthropoda. In: Florkin M, Scheer BT (eds) Chemical zoology, Volume 6, Part B. Academic Press, New York, p 1Google Scholar
  13. Iwanaga T, Fujita T, Nishiitsutsuji-Uwo J, Endo Y (1981) Immunohistochemical demonstration of PP-, somatostatin-, enteroglucagon-, and VIP-like immunoreactivities in the cockroach midgut. Biomed Res 2:202–207Google Scholar
  14. Kramer KJ, Speirs RD, Childs CN (1977) Immunochemical evidence for a gastrin-like peptide in insect neuroendocrine system. Gen Comp Endocrinol 32:423–426Google Scholar
  15. Larsson L-I, Rehfeld JR (1977) Evidence for a common evolutionary origin of gastrin and cholecystokinin. Nature 269:335–338Google Scholar
  16. Larson BA, Vigna SR (1983a) Species and tissue distribution of cholecystokinin/gastrin-like substances in some invertebrates. Gen Comp Endocrinol 50:469–475Google Scholar
  17. Larson BA, Vigna SR (1983b) Gastrin/cholecystokinin-like immunoreactive peptides in the Dungeness crab, Cancer magister (Dana): Immunochemical and biological characterization. Regul Pept 7:155–170Google Scholar
  18. Larson BA Scalise FW, Reeve JR Jr., Vigna SR (1982) Immunochemical characterization and localization of gastrin/cholecystokinin-like immunoreactants in the Dungeness crab, Cancer magister. Am Zool 22:949Google Scholar
  19. Locke M (1976) The role of plasma membrane plaques and Golgi complex vesicles in cuticle deposition during the moult/intermoult cycle. In: Hepburn HR (ed) The insect integument. Elsevier, Amsterdam, p 237Google Scholar
  20. Mykles DL (1979) Ultrastructure of alimentary epithelia of lobsters, Homarus americanus and H. gammarus, and crab, Cancer magister, Zoomorphology 92:201–215Google Scholar
  21. Nishiitsutsuji-Uwo J, Endo Y (1981) Gut endocrine cells in insects: The ultrastructure of the endocrine cells in the cockroach midgut. Biomed Res 2:30–44Google Scholar
  22. Polak JM, Pearse AGE (1970) Anti-gastrin immunofluorescence in the skin of Hyla crepitans and the cytochemistry of the cells involved. Experientia 26:288–289Google Scholar
  23. Rzasa P, Kaloustian KV, Prokop EK (1982) Immunochemical evidence for a gastrin-like peptide in the intestinal tissues of the earthworm Lumbricus terrestris. Comp Biochem Physiol 71A:631–634Google Scholar
  24. Steiner DF, Peterson JD, Tager H, Emdin S, Ostberg Y, Falkmer S (1973) Comparative aspects of proinsulin and insulin structure and biosynthesis. Am Zool 13:591–604Google Scholar
  25. Sternberger LA (1979) Immunocytochemistry, 2nd edition. Wiley, New YorkGoogle Scholar
  26. Straus E, Yalow RS, Gainer H (1975) Molluscan gastrin: concentration and molecular forms. Science 190:687–689Google Scholar
  27. Thorndyke MC (1982) Cholecystokinin(CCK)/gastrin-like immunoreactive neurones in the cerebral ganglion of the protochordate ascidians Styela clava and Ascidiella aspersa. Regul Pept 3:281–288Google Scholar
  28. Van Noorden SE, Pearse AGE (1976) The localization of immunoreactivity to insulin, glucagon and gastrin in the gut of Amphioxus (Branchiostoma) lanceolatus. In: Grillo TAI, Liebson L, Epple A (eds) The evolution of pancreatic islets. Pergamon, New York, p 163Google Scholar
  29. Walsh JH (1981) Gastrointestinal hormones and peptides. In: Johnson LR (ed) Physiology of the gastrointestinal tract. Raven Press, New York, p 59Google Scholar
  30. Walsh JH, Lamers CB, Valenzuela JE (1982) Cholecystokinin-octapeptidelike immunoreactivity in human plasma. Gastroenterology 82:438–444Google Scholar
  31. Warner GF (1977) The biology of crabs. Van Nostrand Reinhold, New York, pp 1–21, 125–127Google Scholar
  32. Zacharuk RY (1976) Structural changes of the cuticle associated with moulting. In: Hepburn HR (ed) The insect integument. Elsevier, Amsterdam, p 299Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Frederick W. Scalise
    • 1
  • Brett A. Larson
    • 1
  • Steven R. Vigna
    • 1
  1. 1.Department of BiologyUniversity of OregonEugeneUSA

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