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Cell and Tissue Research

, Volume 226, Issue 3, pp 511–522 | Cite as

Fine structure and permeability of capillaries in the stria vascularis and spiral ligament of the inner ear of the guinea pig

  • Masafumi Sakagami
  • Toru Matsunaga
  • Paulo H. Hashimoto
Article

Summary

The blood capillaries in the stria vascularis and the spiral ligament of guinea pigs were studied by electron microscopy with freeze-fracture and thin section methods, including tracer experiments with horseradish peroxidase (HRP) and microperoxidase (MP). The endothelial cells of the capillaries of both tissues are connected by tight junctions, and contain about the same number of micropinocytotic vesicles. In cases of intravascular administration before fixation, both of the tracers stained the perivascular space and almost all endothelial vesicles in the stria vascularis. On the other hand, the perivascular space and many vesicles in the spiral ligament were unstained. The endothelial tight junctions in the stria vascularis prevented the penetration of HRP, but sometimes allowed the penetration of MP. Those of the spiral ligament were impermeable to both tracers. In cases of tracer administration after fixation, leakage spots of HRP from capillaries were sparsely located all over the stria vascularis. Transendothelial channels and isolated fenestrae formed by micropinocytotic vesicles were detected. It is concluded that the capillaries of the stria vascularis are similar to the muscle capillaries and to the capillaries of the elasmobranch brain, whereas those in the spiral ligament are similar to the brain capillaries of higher vertebrates.

Key words

Capillary permeability Stria vascularis Spiral ligament Freeze-fracture Inner ear 

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References

  1. Cotran RS, Karnovsky MJ (1967) Vascular leakage induced by horseradish peroxidase in the rat. Proc Soc Exp Biol (NY) 126:557–561Google Scholar
  2. Deurs BV (1977) Vesicular transport of horseradish peroxidase from brain to blood in segments of the cerebral microvasculature in adult mice. Brain Res 124:1–8Google Scholar
  3. Deurs BV, Amtrop O (1978) Blood-brain barrier in rats to the hemepeptide microperoxidase. Neuroscience 3:737–748Google Scholar
  4. Duvall AJ, Quick CA, Sutherland CR (1971) Horseradish peroxidase in the lateral cochlear wall. Arch Otolaryngol 93:304–316Google Scholar
  5. Gorgas K, Jahnke K (1974) The permeability of blood vessels in the guinea pig cochlea. II. Vessels in the spiral ligament and the stria vascularis. Anat Embryol 146:33–42Google Scholar
  6. Graham RC, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–302Google Scholar
  7. Hashimoto PH (1972) Intercellular channels as a route for protein passage in the capillary endothelium of the shark brain. Am J Anat 134:41–58Google Scholar
  8. Hinojosa R, Rodriguez-Echandia EL (1966) The fine structure of the stria vascularis of the cat inner ear. Am J Anat 118:631–664Google Scholar
  9. Jahnke K (1975a) Die Feinstruktur gefriergeätzter Zellmembran-Haftstellen der Stria vascularis. Anat Embryol 147:189–201Google Scholar
  10. Jahnke K (1975b) The fine structure of freeze-fractured intercellular junctions in the guinea pig inner ear. Acta Otolaryngol (Stockh) Suppl 336:1–40Google Scholar
  11. Jahnke K (1980) The blood-perilymph barrier. Arch Otorhinolaryngol 228:29–34Google Scholar
  12. Jahnke K, Gorgas K (1974) The permeability of blood vessels in the guinea pig cochlea. I. Vessels of the modiolus and spiral vessel. Anat Embryol 146:21–31Google Scholar
  13. Karnovsky MJ (1967) The ultrastructural basis of capillary permeability studied with peroxidase as a tracer. J Cell Biol 35:213–236Google Scholar
  14. Kimura Rs, Ota CY (1974) Ultrastructure of the cochlear blood vessels. Acta Otolaryngol (Stockh) 77:231–250Google Scholar
  15. Kimura RS, Schuknecht HF (1970) The ultrastructure of the human stria vascularis. Part I. Acta Otolaryngol (Stockh) 69:415–427Google Scholar
  16. Osako S, Hilding DA (1971) Electron microscopic studies of capillary permeability in normal and Ames Waltzer deaf mice. Acta Otolaryngol (Stockh) 71:365–376Google Scholar
  17. Palade GE, Simionescu M, Simionescu N (1979) Structural aspects of the permeability of the microvascular endothelium. Acta Physiol Scand Suppl 463:11–32Google Scholar
  18. Reale E, Luciano L, Franke K, Pannase E, Wermbter G, Iurato S (1975) Intercellular junctions in the vascular stria and spiral ligament. J Ultrastruct Res 53:284–297Google Scholar
  19. Reese TS, Karnovsky MJ (1967) Fine structural localization of a blood-brain barrier to exogenous peroxidase. J Cell Biol 34:207–217CrossRefPubMedGoogle Scholar
  20. Santos-Sacchi J, Marovitz WF (1980) An evaluation of normal striai capillary transport using the electron-opaque tracers ferritin and iron dextran. Acta Otolaryngol (Stockh) 89:12–26Google Scholar
  21. Simionescu N, Simionescu M, Palade GE (1975a) Permeability of muscle capillaries to small hemepeptides. J Cell Biol 64:586–607Google Scholar
  22. Simionescu M, Simionescu N, Palade GE (1975b) Segmental differentiations of cell junctions in the vascular endothelium. J Cell Biol 67:863–885Google Scholar
  23. Simionescu N, Simionescu M, Palade GE (1978) Open junctions in the endothelium of the postcapillary venules of the diaphragm. J Cell Biol 79:27–44Google Scholar
  24. Takahashi T, Kimura RS (1970) The ultrastructure of the spiral ligament in the Rhesus monkey. Acta Otolaryngol (Stockh) 69:46–60Google Scholar
  25. Tani E, Yamagata S, Ito Y (1977) Freeze-fracture of capillary endothelium in rat brain. Cell Tissue Res 176:157–165Google Scholar
  26. William MC, Wissig SL (1975) The permeability of muscle capillaries to horseradish peroxidase. J Cell Biol 66:531–555Google Scholar
  27. Winther FØ (1971 a) The permeability of the guinea pig cochlear capillaries to horseradish peroxidase. Z Zellforsch 114:193–202Google Scholar
  28. Winther FØ (1971 b) Diffusion barrier to horseradish peroxidase in the vascular stria of the guinea pig. Z Zellforsch 121:499–506Google Scholar
  29. Wissig SL (1979) Identification of the small pore in muscle capillaries. Acta Physiol Scand Suppl 463:33–44Google Scholar
  30. Wissig SL, Williams MC (1978) Permeability of muscle capillaries to microperoxidase. J Cell Biol 76:341–359Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Masafumi Sakagami
    • 1
  • Toru Matsunaga
    • 1
  • Paulo H. Hashimoto
    • 1
  1. 1.Departments of Otolaryngology and AnatomyOsaka, University Medical SchoolOsakaJapan

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