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Journal of Comparative Physiology A

, Volume 172, Issue 1, pp 91–99 | Cite as

Functional and morphological regeneration of olfactory tracts and subtracts in goldfish

  • H. P. Zippel
  • M. Hofmann
  • D. L. Meyer
  • S. Zeman
Article

Abstract

Goldfish are ideal vertebrates for the study of regeneration within the central nervous system. The present behavioural and neuroanatomical investigations after bilateral transection of the entire olfactory tracts of either lateral or medial subtracts have been designed (1) to examine the relationship between morphological changes and changes in the perception of spontaneously preferred chemosensory stimuli, (2) to investigate the animals' ability to qualitatively discriminate amino acids in olfactory concentrations (below taste threshold, 10-6–10-8M), one of which had been rewarded preoperatively (“specific” regeneration), and (3) to examine the discriminative ability for amino acids at concentrations above taste threshold (> 10-5M) in intact sham-operated, and in operated specimens at various time intervals before functional regeneration. Within 10–14 days after bilateral transection of the lateral olfactory tracts, specific regeneration was observed. After bilateral transection of the medial olfactory tracts, no immediate behavioural change was recorded for 1 week. Thereafter, goldfish behaviour became unstable and dropped to the chance level for 3–4 weeks. Subsequent to this time the goldfish returned to the preoperative level. Following bilateral crushing of the olfactory tracts and after total tractotomy, a specific regeneration was observed after 4 weeks and 6–8 weeks, respectively, post op. HRP studies showed that after bilateral lesioning a qualitative reinnervation of the respective nuclei within the forebrain by the medial and lateral olfactory subtracts was evident.

Key words

Regeneration Olfactory tract Behaviour Morphology Goldfish 

Abbreviations

FB

funnel biting

FO

funnel orientation

HRP

horseradish peroxidase

LOT

lateral olfactory tract

MOT

medial olfactory tract

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • H. P. Zippel
    • 1
  • M. Hofmann
    • 2
    • 3
  • D. L. Meyer
    • 2
    • 3
  • S. Zeman
    • 1
  1. 1.Physiologisches Institut der UniversitätGöttingenGermany
  2. 2.Anatomisches Institut der UniversitätGöttingen
  3. 3.Institut für NeurobiologieMagdeburgGermany

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