Contaminant levels in harbor seals from the northeastern United States

  • C. A. Lake
  • J. L. Lake
  • R. Haebler
  • R. McKinney
  • W. S. Boothman
  • S. S. Sadove


The concentrations of polychlorinated biphenyls (PCBs), organochlorine pesticides, polycyclic aromatic hydrocarbons (PAHs), polychlorinated dibenzofurans (PCDF), polychlorinated dibenzo-p-dioxins (PCDD), and mercury (Hg) were determined in blubber and liver tissues of harbor seals (Phoca vitulina) collected along the northeast coast of the U.S. Average PCB concentrations in seal blubber (sum of congeners) were 12.0 μg/g (wet weight) with a range of 7.30 to 24.3 μg/g in 1980 and 6.66 μg/g (wet weight) with a range of 2.61 to 11.3 μg/g in 1990–1992. Comparisons between blubber data from this study and previous work indicated that the concentration of PCBs along the northeast coast of the U.S. may have decreased over the past twenty years.

The average p,p′-DDE concentrations in seal blubber were 10.9 μg/g (wet weight) in 1980 with a range of 6.95 to 21.9 μg/g and 4.12 μg/g (wet weight) with a range of 1.83 to 7.84 μg/g in 1990–1992. Only trace amounts of PCDFs and PCDDs were found in a few blubber samples; levels in most tissues were below detection (3–5 pg/g) (wet weight). Trace amounts (<30 ng/g) of phenanthracene, anthracene, and alkylated MW-178 compounds were found in some seal samples; all other PAH compounds were below the detection level (5–15 ng/g).

Toxic equivalents (TEQ) of selected coplanar and mono-ortho PCB congeners and relative toxic equivalents (RTE) (pg total TEQ/μg total PCB) were calculated, using recently proposed dioxin toxic equivalent factors (Ahlborg et al. 1994). The TEQs ranged from 41 to 315, and the RTEs ranged from 2.25 to 16.3. The RTEs for seal blubber indicated that the present values were in the midrange of those reported in the literature. Toxic equivalents calculated on the basis of the concentrations of the coplanar PCBs, PCDDs, and PCDFs indicated that coplanar PCBs, rather than PCDDs and PCDFs, may pose a more important toxic threat to harbor seals.

Mercury levels in liver tissue averaged 70.0 μg/g (wet weight) and 44.1 μg/g (wet weight) in the 1991 and 1980 samples, respectively, and are similar to those found in relatively polluted waters of the British Isles.


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  1. Addison RF (1989) Organochlorines and marine mammal reproduction. Can J Fish Aquat Sci 46:360–368Google Scholar
  2. Addison RF, Brodie PF, Zinck ME (1984) DDT has declined more than PCBs in eastern Canadian seals during the 1970s. Environ Sci Technol 18:935–937Google Scholar
  3. Ahlborg UG, Becking GC, Birnbaum LS, Brouwer A, Derls HJGM, Felley M, Golor G, Hanberg A, Larsen JC, Liem AKD, Safe SH, Schlatter C, Waern F, Younes M, Yrjanheikki E (1994) Toxic equivalency factors for dioxin-like PCBs. Chemosphere 28(6):1049–1067Google Scholar
  4. Aulerich RJ, Ringer RK (1977) Current status of PCB toxicity to mink and effect on their reproduction. Arch Environ Contam Toxicol 6:279–292Google Scholar
  5. Ballschmitter K, Zell M (1980) Analysis of polychlorinated biphenyls (PCBs) by glass capillary gas chromatography: Composition of technical Arochlor®- and Clophen®-PCB mixtures. Fresenius Zeitschrift Fur Analytische Chemie 302:201–231Google Scholar
  6. Bergek S, Bergqvist P-A, Hjelt M, Olsson M, Rappe C, Roos A, Zook D (1992) Concentrations of PCDDs and PCDFs in seals from Swedish water. Ambio 21:553–556Google Scholar
  7. Bergman A, Backlin B-M, Jarplid B, Grimelius L, Wilander E (1992) Influence of commercial polychlorinated biphenyls and fractions thereof on liver histology in female mink (Mustela vison). Ambio 21:591–595Google Scholar
  8. Blomkvist G, Roos A, Jensen S, Bignert A, Olsson M (1992) Concentrations of sDDT and PCB in seals from Swedish and Scottish waters. Ambio 21:539–545Google Scholar
  9. Boon JP, Eijgenraam F, Duiker JC, Everaarts JM (1989) A structure-activity relationship (SAR) approach towards metabolism of PCBs in marine animals from different trophic levels. Mar Environ Res 27:159–176Google Scholar
  10. Boon JP, Reijnders JH, Dols J, Wensvoort P, Hillebrand MTJ (1987) The kinetics of individual polychlorinated biphenyl congeners in female harbour seals (Phoca vitulina), with evidence for structure-related metabolism. Aquat Toxicol 10:307–324Google Scholar
  11. Brouwer A, Reijnders PJH, Koeman JH (1989) Polychlorinated biphenyl (PCB)-contaminated fish induces vitamin A and thyroid hormone deficiency in the common seal (Phoca vitulina). Aquat Toxicol 15:99–106Google Scholar
  12. Brunstrom B (1992) Induction of cytochrome P-450-dependent enzyme activities in female mink (Mustela vison) and their kits by technical PCB preparations and fractions thereof. Ambio 21:585–587Google Scholar
  13. Daelemans FF, Mehlum F, Lydersen C, Schepens PJC (1993) Mono-ortho and non-ortho substituted PCBs in arctic ringed seal (Phoca hispida) from the Svalbard area: Analysis and determination of their toxic threat. Chemosphere 27:429–437Google Scholar
  14. Engelhardt FR (1982) Hydrocarbon metabolism and corticol balance in oil-exposed ringed seals, Phoca hispida. Comp Biochem Physiol 72C:133–136Google Scholar
  15. Frank A, Galgan V, Roos A, Olsson M, Petersson LR, Bignert A (1992) Metal concentrations in seals from Swedish waters. Ambio 21:529–538Google Scholar
  16. Freeman HC, Horne DA (1973) Mercury in seals. Bull Environ Contam Toxicol 10:172–180Google Scholar
  17. Gaskin DE, Frank R, Holdrinet M, Ishida K, Walton CJ, Smith M (1973) Mercury, DDT, and PCB in harbour seals (Phoca vitulina) from the Bay of Fundy and Gulf of Maine. J Fish Res Board Can 30:471–475Google Scholar
  18. Hakansson H, Manzoor E, Ahlborg UG (1992) Effects of technical PCB preparations and fractions thereof on vitamin A levels in the mink (Mustela vison). Ambio 21:588–590Google Scholar
  19. Haraguchi K, Athanasiadou M, Bergman A, Hovander L, Jensen S (1992) PCB and PCB methyl sulfones in selected groups of seals from Swedish waters. Ambio 21:546–549Google Scholar
  20. Hellou J, Stenson G, Ni I-H, and Payne JF (1990) Polycyclic aromatic hydrocarbons in muscle tissue of marine mammals from the northwest Atlantic. Mar Pollut Bull 21:469–473Google Scholar
  21. Jansson B, Andersson R, Asplund L, Litzen K, Nylund K, Sellstrom U, Uvemo U-B, Wahlberg C, Wideqvost U, Odsj T, Olsson M (1993) Chlorinated and brominated persistent organic compounds in biological samples from the environment. Environ Toxicol Chem 12:1163–1174Google Scholar
  22. Kannan N, Tanabe S, Ono M, Tatsukawa R (1989) Critical evaluation of polychlorinated biphenyl toxicity in terrestrial and marine mammals: Increasing impact of non-ortho and mono-ortho coplanar polychlorinated biphenyls from land to ocean. Arch Environ Contam Toxicol 18:850–857Google Scholar
  23. Kihlsrom JE, Olsson M, Jensen S, Johansson A, Ahlbom J, Bergman A (1992) Effects of PCB and different fractions of PCB on the reproduction of the mink (Mustela vison). Ambio 21:563–569Google Scholar
  24. Koistinen J (1990) Residues of planar polychloroaromatic compounds in Baltic fish and seal. Chemosphere 20:1043–1048Google Scholar
  25. Law RJ, Allchin CR, Harwood J (1989) Concentrations of organochlorine compounds in the blubber of seals from eastern and northeastern England, 1988. Mar Pollut Bull 20:110–115Google Scholar
  26. Law RJ, Filemand CF, Hopkins AD, Baker JR, Harwood J, Jackson DB, Kennedy S, Martin AR, Morris RJ (1991) Concentrations of trace metals in the livers of marine mammals (seals, porpoises and dolphins) from waters around the British Isles. Mar Pollut Bull 22:183–191Google Scholar
  27. Law RJ, Jones BR, Baker JR, Kennedy S, Milne R, Morris RJ (1992) Trace metals in the livers of marine mammals from the Welsh coast and the Irish Sea. Mar Pollut Bull. 24:296–304Google Scholar
  28. Lukas B, Vetter W, Fischer P, Heidemann G, Plotz J (1990) Characteristic chlorinated hydrocarbon patterns in the blubber of seals from different marine regions. Chemosphere 21:13–19Google Scholar
  29. Morris RJ, Law RJ, Allchin CR, Kelly CA, Fileman CF (1989) Metals and organochlorines in dolphins and porpoises of Cardigan Bay, West Wales. Mar Pollut Bull 20:512–523Google Scholar
  30. Mullin M (1987) Congener PCB methodology on-column system. U.S. Environmental Protection Agency, Large Lake Research Station, 9311 Groh Rd., Grosse Ile, MIGoogle Scholar
  31. Munns WR, Jr, Meuller C, Cobbs DJ, Gleason TR, Pesch GG, Johnson RK (1991) Marine ecological risk assessment at Naval Construction Battalion Center, Davisville, RI, Phase I. Technical Report 1437:26–31. Naval Ocean Systems Center, San Diego, CA 92152–5000Google Scholar
  32. Oehme M, Furst P, Kruger C, Meemken HA, Groebel W (1988) Presence of polychlorinated dibenzo-p-dioxins, dibenzofurans, and pesticides in arctic seal from Spitzbergen. Chemosphere 17:1291–1300Google Scholar
  33. Paasivirta J, Rantio T (1991) Chloroterpenes and other organochlorines in Baltic, Finnish, and Arctic wildlife. Chemosphere 22:47–55Google Scholar
  34. Pruell RJ, Lake JL, Davis WR, Quinn JG (1986) Uptake and depuration of organic contaminants by blue mussels, Mytilus edulis, exposed to environmentally contaminated sediments. Mar Biol 91:497–507Google Scholar
  35. Pruell RJ, Norwood C, Bowman RD, Boothman WS, Rogerson PF, Hackett M, Butterworth BC (1990) Geochemical study of sediment contamination in New Bedford Harbor, Massachusetts. Mar Environ Res 29:77–101Google Scholar
  36. Pruell RJ, Rubinstein NI, Taplin BK, Livolsi JA, Bowen RD (1993) Accumulation of polychlorinated organic contaminants from sediment by three benthic marine species. Arch Environ Contam Toxicol 24:290–297Google Scholar
  37. Reijnders PJ (1980) Organochlorine and heavy metal residues in harbour seals from the Wadden Sea and their possible effects on reproduction. Neth J Sea Res 14:30–65Google Scholar
  38. Reijnders PJ (1986) Reproductive failure in common seals feeding on fish from polluted coastal waters. Nature 324:456–457Google Scholar
  39. Safe S (1984) Polychlorinated biphenyls (PCBs) and polybrominated biphenyls (PBBs): Biochemistry, toxicology, mechanism of action. CRC Crit Rev Toxicol 13:319–395Google Scholar
  40. Sergeant DE, Armstrong FAJ (1973) Mercury in seals from eastern Canada. J Fish Res Board Can 30:843–846Google Scholar
  41. Storr-Hansen E, Spliid H (1993) Distribution patterns of polychlorinated biphenyl congeners in harbor seal (Phoca vitulina) tissues: Statistical analysis. Arch Environ Contam Toxicol 25:328–345Google Scholar
  42. Subramanian AN, Tanabe S, Tatsukawa R, Saito S, Miyazaki N (1987) Reduction in the testosterone levels by PCBs and DDE in Dall's porpoises of northwestern North Pacific. Mar Pollut Bull 18:643–646Google Scholar
  43. Tanabe S, Watanabe S, Kan H, Tatsukawa R (1988) Capacity and mode of PCB metabolism in small cetaceans. Mar Mammal Sci 4:103–124Google Scholar
  44. USEPA (1989) Interim procedures for estimating risks associated with exposures to mixtures of chlorinated dibenzo-p-dioxins and-dibenzofurans (CDDs and CDFs) and 1989 update: Risk assessment forum. U.S. Environmental Protection Agency. EPA/625/3-89/016Google Scholar
  45. Yamamoto Y, Honda K, Hidaka H, Tatsukawa R (1987) Tissue distribution of heavy metals in Weddell seals (Leptonychotes weddellii). Mar Pollut Bull 18:164–169Google Scholar

Copyright information

© Springer-Verlag New York Inc 1995

Authors and Affiliations

  • C. A. Lake
    • 1
  • J. L. Lake
    • 2
  • R. Haebler
    • 2
  • R. McKinney
    • 1
  • W. S. Boothman
    • 2
  • S. S. Sadove
    • 3
  1. 1.c/o U.S. Environmental Protection AgencyScience Applications International CorporationNarragansettUSA
  2. 2.Environmental Research LaboratoryU. S. Environmental Protection AgencyNarragansettUSA
  3. 3.OKEANOS Ocean Research Foundation IncHampton BaysUSA

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