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Immunogenetics

, Volume 34, Issue 1, pp 28–38 | Cite as

Developmental and tissue-specific expression of the Q5 k gene

  • Sabine Schwemmle
  • Dorian Bevec
  • Gottfried Brem
  • Manuela B. Urban
  • Patrick A. Baeuerle
  • Elisabeth H. Weiss
Original Articles

Abstract

Expression of the Q5 k gene was examined by northern blot analysis and polymerase chain reaction (PCR) in the AKR mouse and various cell lines, each of the H-2 k haplotype. Our results show that Q5 k mRNA is present during the whole postimplantational development of the AKR embryo/fetus (gestation day 6 to 15). In the juvenile mouse (week 2 to 4) transcription of the Q5 k gene persisted in all organs examined. In contrast, in the adult animal expression of the Q5 k gene was limited to the thymus and uterus of the pregnant mouse. Upon malignant transformation, the amount of Q5 k -specific mRNA increased dramatically in thymus and could also be observed in the spleen of thymoma bearing animals. Expression of the Q5 k gene was also detectable in several transformed mouse cell lines. Mitogen stimulation or treatment with cytokines induced Q5 k expression in primary spleen cell cultures. A possible explanation for the tissue-restricted expression in the adult AKR mouse is discussed.

Keywords

Northern Blot Malignant Transformation Northern Blot Analysis Spleen Cell Thymoma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Sabine Schwemmle
    • 1
  • Dorian Bevec
    • 1
  • Gottfried Brem
    • 2
  • Manuela B. Urban
    • 3
  • Patrick A. Baeuerle
    • 3
  • Elisabeth H. Weiss
    • 1
  1. 1.Institut für ImmunologieMünchenFederal Republic of Germany
  2. 2.Lehrstuhl für Molekulare TierzuchtMünchenFederal Republic of Germany
  3. 3.Genzentrum, Laboratorium für Molekulare BiologieMartinsriedFederal Republic of Germany

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