Immunogenetics

, Volume 34, Issue 5, pp 286–292 | Cite as

An HLA-DRα promoter DNA-binding protein is expressed ubiquitously and maps to human chromosomes 22 and 5

  • Hsiou-Chi Liou
  • Roger Eddy
  • Thomas Shows
  • Barbara Lisowska-Grospierre
  • Claude Griscelli
  • Carolyn Doyle
  • Josef Mannhalter
  • Martha Eibl
  • Laurie H. Glimcher
Original Articles

Abstract

The class II major histocompatibility complex antigens are a family of integral membrane proteins whose expression is tissue-specific and developmentally regulated. Three consensus sequences, X1, X2, and Y, separated by an interspace element, is found upstream from all class II genes. Deletion of each of these sequences eliminates expression of class II genes in vitro or in transgenic mice. Here we further characterize the expression of a cDNA encoding a DNA binding protein (human X-box binding protein, hXBP-1) which, like the proteins in whole nuclear extract, recognizes both the X2 promoter element of the human DRα and DPβ and mouse Aα genes. The hXBP-1 cDNA hybridizes to human RNA species of approximately 2.2 kilobases (kb) and 1.6 kb, which are expressed in class II negative as well as class II positive cells. hXBP-1 transcripts are present in several class II deficient mutant B cell lines, although in one such line, 6.1.6, levels were somewhat reduced. Chromosome mapping studies demonstrate that hXBP-1 arises from a small gene family, two of whose members map to human chromosomes 5 and 22. Taken together, these data suggest a high degree of complexity in the transcriptional control of the class II gene family.

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Hsiou-Chi Liou
    • 1
  • Roger Eddy
    • 4
  • Thomas Shows
    • 4
  • Barbara Lisowska-Grospierre
    • 5
  • Claude Griscelli
    • 5
  • Carolyn Doyle
    • 3
  • Josef Mannhalter
    • 6
  • Martha Eibl
    • 6
  • Laurie H. Glimcher
    • 1
    • 2
  1. 1.Department of Cancer BiologyHarvard School of Public HealthBostonUSA
  2. 2.Department of MedicineHarvard Medical SchoolBostonUSA
  3. 3.Dana Farber Cancer CenterHarvard Medical SchoolBostonUSA
  4. 4.Roswell Park Memorial InstituteBuffaloUSA
  5. 5.Hôpital Enfants MaladesParisFrance
  6. 6.Vienna Medical SchoolViennaAustria
  7. 7.Department of Cancer BiologyHarvard School of Public HealthBostonUSA

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