Theoretical and Applied Genetics

, Volume 86, Issue 8, pp 959–963 | Cite as

Use of RFLP markers for the identification of alleles of the Pm3 locus conferring powdery mildew resistance in wheat (Triticum aestivum L.)

  • L. Hartl
  • H. Weiss
  • F. J. Zeller
  • A. Jahoor


The objective of this study was to identify molecular markers linked to genes for resistance to powdery mildew (Pm) in wheat using a series of ‘Chancellor’ near-isogenic-lines (NILs), each having one powdery mildew resistance gene. A total of 210 probes were screened for their ability to detect polymorphism between the NILs and the recurrent parent. One of these restriction fragment length polymorphism (RFLP) markers (Xwhs179) revealed polymorphism not only between the NILs for the Pm3 locus, but also among NILs possessing different alleles of the Pm3 locus. The location of the marker Xwhs179 was confirmed to be on homoeologous chromosome group 1 with the help of nullitetrasomic wheat lines. The linkage relationship between this probe and the Pm3 locus was estimated with double haploid lines derived from a cross between wheat cvs ‘Club’ and ‘Chul’ (Pm3b). The genetic distance was determined to be 3.3±1.9 cM.

Key words

Triticum aestivum Mildew resistance Pm3 locus Near-isogenic lines RFLP marker 


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  1. Allard RW (1956) Formulas and tables to facilitate the calculation of recombination values in heredity. Hilgardia 24:235–279Google Scholar
  2. Aslam M, Schwarzbach E (1980) An inoculation technique for quantitative studies of brown rust resistance in barley. Phytopathol Z 99:87–91Google Scholar
  3. Briggle LW (1969) Near-isogenic lines of wheat with genes for resistance to Erysiphe graminis f. sp. tritici. Crop Sci 9:70–72Google Scholar
  4. Briggle LW, Sears ER (1966) Linkage of resistance to Erysiphe graminis f. sp. tritici (Pm3) and hairy glume (Hg) on chromosome 1A of wheat. Crop Sci 6:559–561Google Scholar
  5. Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction fragments to high specific activity. Anal Biochem 132:6–13PubMedGoogle Scholar
  6. Felsenstein FG (1991) Virulenz and Fungizidsensitivität des Weizenmehltaus Erysiphe graminis DC f. sp. tritici Marchal, in Europa. PhD thesis, Technical University MunichGoogle Scholar
  7. Flor HH (1955) Host-parasite interaction in flax rust — its genetics and other implications. Phytopathology 45:680–685Google Scholar
  8. Heidecker G, Chaudhuri S, Messing J (1991) Highly clustered zein gene sequences reveal evolutionary history of the multigene family. Genomics 10:719–732Google Scholar
  9. Hentrich W (1979) Allelwirkung und Pleiotropie mehltauresistenter Mutanten des mlo Locus der Gerste. Arch Zuechtungsforsch 9:283–291Google Scholar
  10. Hinze K, Thompson RD, Ritter E, Salamini F, Schulze-Lefert P (1991) RFLP-mediated targeting of the ml-o resistance locus in barley (Hordeum vulgare). Proc Natl Acad Sci USA 88:3691–3695Google Scholar
  11. Ho JJ, Weide R, Ma MM, van Wordvagen F, Lambert KN, Koorneef M, Zabel P, Williamson VM (1992) The root-knot nematode resistance gene (Mi) in tomato: construction of a molecular linkage map and identification of dominant cDNA markers in resistant genotypes. Plant J 2:971–982Google Scholar
  12. Holmes DS, Quigley M (1981) A rapid boiling method for preparation of bacterial plasmids. Anal Biochem 114:193–197Google Scholar
  13. Islam MR, Shepherd KW, Mayo GME (1989) Recombination among genes at the L group in flax conferring resistance to rust. Theor Appl Genet 77:540–546Google Scholar
  14. Jahoor A, Fischbeck G (1987) Genetical studies of resistance of powdery mildew in barley lines derived from Hordeum spontaneum collected from Israel. Plant Breed 99:265–273Google Scholar
  15. Jahoor A, Fischbeck G (1993) Identification of new genes for mildew resistance of barley at the Mla locus in lines derived from Hordeum spontaneum. Plant Breed 110:116–122Google Scholar
  16. Jahoor A, Ludwig A, Fischbeck G (1989) New genes for powdery mildew resistance in Hordeum spontaneum-derived lines allelic or closely linked to the Mlp locus. Barley Genet Newsl 19:23–26Google Scholar
  17. Jung C, Koch R, Fischer F, Brandes G, Wricke G, Herrmann RG (1992) DNA markers closely linked to nematode resistance genes in sugarbeet (Beta vulgaris L.) using chromosome additions and translocations originating from wild beets of the Procumbentes species. Mol Gen Genet 232:271–278Google Scholar
  18. Klein-Lankhorst R, Rietvelt P, Machiels B, Verkerk R, Weide R, Gebhardt C, Koorneef M, Zabel P (1991) RFLP markers linked to the root knot nematode resistance gene Mi in tomato. Theor Appl Genet 81:661–667Google Scholar
  19. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175Google Scholar
  20. Kreis M, Shewry PR, Forde BG, Forde J, Miflin BJ (1985) Structure and evolution of seed storage proteins and their genes with particular reference to those of wheat, barley and rye. Oxford Surv Plant Mol Cell Biol 2:25–317Google Scholar
  21. Lander ES, Green J, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newbury L (1987) Mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181PubMedGoogle Scholar
  22. Leonhard-Shippers C, Gieffers W, Salamini F, Gebhardt C (1992) The R1 gene conferring race-specific resistance to Phytophtora infestans in potato is located on potato chromosome V. Mol Gen Genet 233:278–283Google Scholar
  23. Lutz J (1991) Mehltauresistenz in Aegilops squarrosa L. — Evaluierung, Gentransfer sowie Expression and Vererbung in synthetischen Weizenlinien. PhD thesis, Technical University MunichGoogle Scholar
  24. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.Google Scholar
  25. Manners JM, Davidson AD, Scott KJ (1985) Patterns of noninfectional protein synthesis in barley carrying different genes for resistance to powdery mildew. Plant Mol Biol 4:275–283Google Scholar
  26. Martin GB, Williams JGK, Tanksley SD (1991) Rapid identification of markers linked to Pseudomonas resistance gene in tomato by using random primers and near-isogenic lines. Proc Natl Acad Sci USA 88:2336–2340Google Scholar
  27. McIntosh RA (1988) Catalogue of symbols. In: Miller TE, Koebner RMD (eds) Proc 7th Int Wheat Genet Symp. Bath press, Bath, UKGoogle Scholar
  28. McIntosh RA, Baker EP (1969) Chromosome location and linkage studies involving the Pm3 locus for powdery mildew resistance in wheat. Proc Linn Soc NSW 93:232–238CrossRefPubMedGoogle Scholar
  29. McMullen MD, Louie R (1989) The linkage of molecular markers to a gene controlling the symptom response in maize dwarf mosaic virus. Mol Plant Microb Interactions 2:309–314Google Scholar
  30. Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions using segregating populations. Proc Natl Acad Sci USA 88:9828–9832PubMedGoogle Scholar
  31. Moseman JG (1959) Host-pathogen interaction of the genes for resistance in Hordeum vulgare and for pathogenicity in Erysiphe graminis f. sp. hordei. Phytopathology 49:469–472Google Scholar
  32. Orkin SH (1986) Reverse genetics and human disease. Cell 47:845–850Google Scholar
  33. Saghai-Maroof MA, Solimann KM, Jorgensen RA, Allard RW (1984) Ribosomal spacerlength polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci USA 81:8014–8018PubMedGoogle Scholar
  34. Sarfatti M, Kuten J, Fluhr R, Zanin D (1989) An RFLP marker in tomato linked to the Fusarium oxysporum resistance gene 12. Theor Appl Genet 78:755–759Google Scholar
  35. Schüller C, Backes G, Fischbeck G, Jahoor A (1992) RFLP markers to identify the alleles on the Mla locus conferring powdery mildew resistance in barley. Theor Appl Genet 84:330–338Google Scholar
  36. Sears (1966) Nullisomic-tetrasomic combinations in hexaploid wheat. In: Riley R, Lewis KR (eds) Chromosome manipulation and plant genetics. Suppl. Heredity, pp 29–45Google Scholar
  37. Shewry PR, Parmar S, Franklin J, Burgess SR (1990) Analysis of a rare recombination event within the multigenic Hor2 locus of barley (Hordeum vulgare L.). Genet Res 55:171–176Google Scholar
  38. Siedler H, Graner A (1991) Construction of physical maps of the Hor1 locus of two barley cultivars by pulsed field gel electrophoresis. Mol Gen genet 226:177–181Google Scholar
  39. Sørensen MB (1989) Mapping of the Hor2 locus in barley by pulsed field gel electrophoresis. Carlsberg Res Commun 54:109–120Google Scholar
  40. Starling TM, Roane CW, Camper HM (1984) Registration of ‘Tyler’ wheat. Crop Sci 24:827Google Scholar
  41. Starling TM, Roane CW, Camper HM (1986) Registration of ‘Saluda’ wheat. Crop Sci 26:200Google Scholar
  42. Steinmüller K, Apel K (1986) A simple and efficient procedure for plant chromatin which is suitable for studies of DNaseI-sensitive domains and hypersensitive sites. Plant Mol Biol 7:87–94Google Scholar
  43. Young ND, Zamir D, Ganal MW, Tanksley SD (1988) Use of isogenic lines and simultaneous probing to identify DNA markers tightly linked to the Tm2a gene in tomato. Genetics 120:579–585Google Scholar
  44. Yu ZH, Mackill DJ, Bonman JM, Tanksley SD (1991) Tagging genes for blast resistance in rice via linkage to RFLP markers. Theor Appl Genet 81:471–476Google Scholar
  45. Zeller FJ, Lutz J, Stephan U (1993) Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L.) 1. Mlk and other alleles at the Pm3 locus. Euphytica (in press)Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • L. Hartl
    • 1
  • H. Weiss
    • 1
  • F. J. Zeller
    • 1
  • A. Jahoor
    • 1
  1. 1.Lehrstuhl fuer Pflanzenbau und PflanzenzüchtungTechnical University MunichFreising-WeihenstephanGermany

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