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Theoretical and Applied Genetics

, Volume 86, Issue 8, pp 935–942 | Cite as

A rice protein library: a data-file of rice proteins separated by two-dimensional electrophoresis

  • S. Komatsu
  • H. Kajiwara
  • H. Hirano
Article

Abstract

Proteins extracted from embryos, endosperms and leaves of rice were separated by two-dimensional electrophoresis and relative molecular weights and isoelectric points were determined. The separated proteins were electroblotted onto a polyvinylidene difluoride membrane and 85 electroblotted proteins were analyzed by a gas-phase protein sequencer. The N-terminal amino-acid sequences of 27 out of 85 proteins were determined in this manner. The N-terminal regions of the remaining proteins could not be sequenced and they were inferred to have a blocking group at the N-terminus. Among proteins, 11 could be sequenced after deblocking by in situ treatment with pyroglutamyl peptidase. The internal amino-acid sequences of 23 proteins were determined by sequence analysis of peptides obtained by Cleveland peptide mapping. The amino-acid sequences determined here were compared with those of known plant and animal proteins. The concanavalin A-peroxidase method was used to determine whether the 85 proteins were glycosylated and the diagonal electrophoresis method was used to determine whether they contained disulphide bonding. Finally, we constructed a data-file of rice proteins including information on relative molecular weight, isoelectric point, amino-acid sequence, sequence homology, glycosylation, and the presence of disulphide bonding.

Key words

Rice Embryo proteins Endosperm proteins Leaf proteins Protein database 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • S. Komatsu
    • 1
  • H. Kajiwara
    • 1
  • H. Hirano
    • 1
  1. 1.Department of Molecular BiologyNational Institute of Agrobiological ResourcesIbarakiJapan

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