Culture of transformed horseradish roots as a source of fusicoccin-like ligands

  • Alexey V. Babakov
  • Lidiya M. Bartova
  • Irina L. Dridze
  • Alexandr N. Maisuryan
  • Gallina U. Margulis
  • Robert R. Oganian
  • Vera D. Voblikova
  • Georgy S. Muromtsev
Article
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Accepted:

Abstract

Endogenous fusicoccin (FC) or related substances were sought in horseradish (Armoracia rusticana P.) roots. An actively growing root culture was derived from plants transformed with Agrobacterium rhizogenes. The presence of FC-like substances in ethanolic extracts from roots was established in a radioreceptor binding assay with plasmalemmal FC receptors and in radioimmune analysis with an antiserum specific for FC A. FC-like ligands were found in the tissue and medium of aseptically grown culture.

Keywords

Gibberellin Endogenous Ligand Agrobacterium Rhizogenes High Performance Liquid Chroma Russian Plant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

FC

fusicoccin

GC/MS

gas chromatography/mass spectrometry

RIA

radioimmunoassay

RRA

radioreceptor analysis

BSA

bovine serum albumin

Mes

4-morpholineethanesulfonic acid

HPLC

high performance liquid chromatography

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Copyright information

© Springer-Verlag New York Inc 1995

Authors and Affiliations

  • Alexey V. Babakov
    • 1
  • Lidiya M. Bartova
    • 1
  • Irina L. Dridze
    • 1
  • Alexandr N. Maisuryan
    • 1
  • Gallina U. Margulis
    • 1
  • Robert R. Oganian
    • 1
  • Vera D. Voblikova
    • 1
  • Georgy S. Muromtsev
    • 1
  1. 1.Research Institute of Agricultural BiotechnologyMoscow

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