Biosynthesis of brassinolide from teasterone via typhasterol and castasterone in cultured cells of Catharanthus roseus

  • Hiroyuki Suzuki
  • Shozo Fujioka
  • Suguru Takatsuto
  • Takao Yokota
  • Noboru Murofushi
  • Akira Sakurai
Article
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Accepted:

Abstract

The biosynthesis of brassinolide (BL) in crown gall and nontransformed cells of Catharanthus roseus in which BL, castasterone (CS), typhasterol (TY), and teasterone (TE) are endogenous was investigated using deuterated TY and TE as substrates. The metabolites were analyzed by gas chromatography-mass spectrometry (GC-MS) and/or GC-selected ion monitoring (SIM). It was found that these cells converted TY to CS and BL, as well as TE to TY and CS. Because the pathway from CS to BL in the cells has already been confirmed, a biosynthetic sequence of TE → TY → CS → BL was established. Reversible conversion between TE and TY was observed.

Keywords

High Performance Liquid Chromatography V208 Cell Brassinolide Nontransformed Cell Castasterone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag New York Inc. 1994

Authors and Affiliations

  • Hiroyuki Suzuki
    • 2
  • Shozo Fujioka
    • 1
  • Suguru Takatsuto
    • 3
  • Takao Yokota
    • 4
  • Noboru Murofushi
    • 2
  • Akira Sakurai
    • 1
  1. 1.The Institute of Physical and Chemical Research (RIKEN)SaitamaJapan
  2. 2.Department of Agricultural ChemistryThe University of TokyoTokyoJapan
  3. 3.Department of ChemistryJoetsu University of EducationNiigataJapan
  4. 4.Department of BiosciencesTeikyo UniversityUtsunomiyaJapan

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