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Theoretical and Applied Genetics

, Volume 80, Issue 3, pp 333–336 | Cite as

5S-RNA genes of barley are located on the second chromosome

  • A. Kolchinsky
  • V. Kanazin
  • E. Yakovleva
  • A. Gazumyan
  • C. Kole
  • E. Ananiev
Originals

Summary

The genes coding for 5S RNA in barley were cloned, sequenced, and their cluster was assigned to chromosome 2 using wheat-barley chromosome addition lines. High-resolution gel-electrophoresis of DNA and subsequent hybridization revealed new details of the organization of 5S DNA both in wheat and barley. The in situ hybridization of the cloned 5S gene with triploid endosperm nuclei also suggests that these genes are located in a single locus.

Key words

5S gene Barley Wheat Addition lines In situ hybridization 

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References

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • A. Kolchinsky
    • 1
  • V. Kanazin
    • 1
  • E. Yakovleva
    • 1
  • A. Gazumyan
    • 1
  • C. Kole
    • 1
  • E. Ananiev
    • 1
  1. 1.Laboratory of Plant Molecular GeneticsN. I. Vavilov Institute of General Genetics of the USSR Academy of SciencesMoscowUSSR

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