Human Genetics

, Volume 84, Issue 2, pp 147–150 | Cite as

Regional assignment of 41 human DNA fragments on chromosome 7 by means of a somatic cell hybrid panel

  • Anke Jobs
  • Dirk Klein-Bölting
  • Almuth-Sigrun Jandel
  • Albert Driesel
  • Klaus Olek
  • Karl-Heinz Grzeschik
Original Investigations


To detect new restriction fragment length polymorphisms that would cover human chromosome 7 with a network of genetic landmarks, a chromosome 7-specific phage gene library was screened for human single-copy fragments. With use of a somatic cell hybrid panel containing defined regions of human chromosome 7, 41 cloned human single-copy sequences were assigned to five regions of this chromosome. Of special importance are the cell hybrid clones GM1059Rag5 and 7851Rag10-1, derived from human cells with interstitial deletions spanning the bands 7q22-q32, within which the cystic fibrosis gene is located. Twelve new probes are described in 7q22-q32, five of which detect a total of six RFLPs.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bartels I, Grzeschik K-H, Cooper DN, Schmidtke J (1986) Regional mapping of six cloned DNA sequences on human chromosome 7. Am J Hum Genet 38:280–287Google Scholar
  2. Bauch W, Hellkuhl B, Grzeschik K-H (1978) Regional assignment of the gene for human beta-glucuronidase by the use of human mouse cell hybrids. Cytogenet Cell Genet 22:434–436Google Scholar
  3. Beaudet A, Bowcock A, Buchwald M, Cavalli-Sforza L, Farrall M, King M-C, Klinger K, Lalouel J-M, Lathrop N, Naylor S, Tsui L-C, Wainwright B, Watkins P, White R, Williamson R (1986) Linkage of cystic fibrosis to two tightly linked DNA markers: joint report from a collaborative study. Am J Hum Genet 39:681–693Google Scholar
  4. Bender K, Grzeschik K-H (1976) Possible assignment of the glyoxylase I (GLO) gene to chromosome 6 using man-mouse somatic cell hybrids. Hum Genet 31:341–345Google Scholar
  5. Benton WD, Davies RW (1977) Screening lambda gt recombinant clones by hybridization to single plaques in situ. Science 196:180Google Scholar
  6. Croce CM, Koprowski H (1974) Somatic cell hybrids between mouse peritoneal macrophages and SV 40 transformed human cells. I. Positive control of the transformed phenotype by the human chromosome 7 carrying the SV 40 genome. J Exp Med 139:1350–1353Google Scholar
  7. Davies KA, Lorand L, Waterfield M, Wainwright B, Farrall M, Williamson R (1987) Isolation of a polymorphic genomic clone from chromosome 7. Hum Genet 77:122–126Google Scholar
  8. Deaven L, Van Dilla M, Bartholdi MF, Carrano AV, Cram LS, Fuscoe JC, Gray JW, Hildebrand CE, Moyzis RK, Perlman J (1986) Construction of human chromosome-specific DNA libraries from flow-sorted chromosomes. Cold Spring Harbor Symp Quant Biol 51:159–167Google Scholar
  9. Estivill X, Farrall M, Scambler PJ, Bell GM, Hawley KMF, Lench NJ, Bates GP, Kruyer HC, Frederick PA, Stanier P, Watson EK, Williamson R, Wainwright BJ (1987) A candidate for the cystic fibrosis locus isolated by selection for methylation-free islands. Nature 326:820–845Google Scholar
  10. Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13PubMedGoogle Scholar
  11. Feinberg AP, Vogelstein B (1984) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Addendum. Anal Biochem 137:266–267PubMedGoogle Scholar
  12. Higginson G, Weaver DD, Magenis RE, Prescott GH, Haag C, Hepburn DJ (1976) Interstitial deletion of the long arm of chromosome no. 7 (7q) in an infant with multiple anomalies. Clin Genet 10:307–312Google Scholar
  13. Kere J, Ruutu T, Chapelle A de la (1987) Monosomy 7 in granulocytes and monocytes in myelodysplastic syndrome. N Engl J Med 316:499–503Google Scholar
  14. Klep-de Pater JM, Bijlsma JB, Bleeker-Wagemakers EM, De France HF, De Vries-Ekkers CMAM (1979) Two cases with different deletions of the long arm of chromosome 7. J Med Genet 16:151–154Google Scholar
  15. Klinger K, Winquist R, Riccio A, Andreasen PA, Sartorio R, Nielsen LS, Stuart N, Stanislovitis P, Watkins P, Douglas R, Grzeschik K-H, Alitalo K, Blasi F, Dano K (1987) Plasminogen activator inhibitor type 1 gene is located at region q21.3-q22 of chromosome 7 and genetically linked with cystic fibrosis. Proc Natl Acad sci USA 84:8548–8552Google Scholar
  16. Lathrop GM, Farrall M, O'Connell P, Wainwright B, Leppert M, Nakamura Y, Lench N, Kruyer H, Dean M, Park M, Vande Woude G, Lalouel J-M, Williamson R, White R (1988) Refined linkage map of chromosome 7 in the region of the cystic fibrosis gene. Am J Hum Genet 42:38–44Google Scholar
  17. Murray JAH (1986) HCC ligation: rapid and specific DNA construction with blunt end DNA fragments. Nucleic Acids Res 14:10118Google Scholar
  18. Naylor SL, Sakaguchi AY, Shows TB, Grzeschik K-H, Holmes M, Zasloff M (1983) Two nonallelic tRNA j/met genes are located in the p23 to q12 region of human chromosome 6. Proc Natl Acad sci USA 80:5027–5031Google Scholar
  19. Scambler PJ, Wainwright BJ, Watson E, Bates G, Bell G, Williamson R, Farrall M (1986) Isolation of a further anonymous DNA sequence from chromosome seven closely linked to cystic fibrosis. Nucleic Acids Res 14:1951–1956Google Scholar
  20. Shows TB, Brown JA, Haley LL, Byers MG, Eddy RL, Cooper ES, Goggin AP (1978) Assignment of the beta-glucuronidase structural gene to the pter-q22 region of chromosome 7 in man. Cytogenet Cell Genet 21:99–104Google Scholar
  21. Southern E (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517PubMedGoogle Scholar
  22. Spence MA, Tsui L-C (1987) Report of the committee of the genetic constitution of chromosomes 7, 8 and 9. (9th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 46:170–187Google Scholar
  23. Tommerup N, Nielsen F (1983) A familial reciprocal translocation t(3;7)(p21.1;p13) associated with Greig polysyndactyly-cranio-facial anomalies syndrome. Am J Med Genet 16:313–321Google Scholar
  24. Tsui L-C, Plavsic N, Markewicz D, Zsiga M, Kennedy D, Zengerling S, Geller S, Hajjan Z, Kuper S, Panak H, Covic L, Rozmahal R, Buchwald M (1987) Molecular approaches to the cystic fibrosis gene. In: Riordan JR, Buchwald M (eds) Genetics and epithelial cell dysfunction in cystic fibrosis. Liss, New York, pp 73–87Google Scholar
  25. Wainwright BJ, Scambler PJ, Williamson R (1987) Regional localization of three probes closely linked to the cystic fibrosis locus by deletion analysis. Cytogenet Cell Genet 44:101–102Google Scholar
  26. Zengerling S, Olek K, Tsui L-C, Grzeschik K-H, Riordan JR, Buchwald M (1987) Mapping of DNA markers linked to the cystic fibrosis locus on the long arm of chromosome 7. Am J Hum Genet 40:228–236Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Anke Jobs
    • 1
  • Dirk Klein-Bölting
    • 2
  • Almuth-Sigrun Jandel
    • 3
  • Albert Driesel
    • 4
  • Klaus Olek
    • 5
  • Karl-Heinz Grzeschik
    • 1
  1. 1.Institut für Humangenetik der UniversitätMarburgGermany
  2. 2.Institut für Biochemie der PflanzenMünsterGermany
  3. 3.Institut für Humangenetik der UniversitätMünsterGermany
  4. 4.Dechema Institut FrankfurtFrankfurtGermany
  5. 5.Institut für Klinische Biochemie der UniversitätBonn, 1Germany

Personalised recommendations