Human Genetics

, Volume 84, Issue 2, pp 147–150 | Cite as

Regional assignment of 41 human DNA fragments on chromosome 7 by means of a somatic cell hybrid panel

  • Anke Jobs
  • Dirk Klein-Bölting
  • Almuth-Sigrun Jandel
  • Albert Driesel
  • Klaus Olek
  • Karl-Heinz Grzeschik
Original Investigations

Summary

To detect new restriction fragment length polymorphisms that would cover human chromosome 7 with a network of genetic landmarks, a chromosome 7-specific phage gene library was screened for human single-copy fragments. With use of a somatic cell hybrid panel containing defined regions of human chromosome 7, 41 cloned human single-copy sequences were assigned to five regions of this chromosome. Of special importance are the cell hybrid clones GM1059Rag5 and 7851Rag10-1, derived from human cells with interstitial deletions spanning the bands 7q22-q32, within which the cystic fibrosis gene is located. Twelve new probes are described in 7q22-q32, five of which detect a total of six RFLPs.

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Anke Jobs
    • 1
  • Dirk Klein-Bölting
    • 2
  • Almuth-Sigrun Jandel
    • 3
  • Albert Driesel
    • 4
  • Klaus Olek
    • 5
  • Karl-Heinz Grzeschik
    • 1
  1. 1.Institut für Humangenetik der UniversitätMarburgGermany
  2. 2.Institut für Biochemie der PflanzenMünsterGermany
  3. 3.Institut für Humangenetik der UniversitätMünsterGermany
  4. 4.Dechema Institut FrankfurtFrankfurtGermany
  5. 5.Institut für Klinische Biochemie der UniversitätBonn, 1Germany

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