Alterations in life-history traits of Chironomus riparius (diptera) obtained from metal contaminated rivers

  • J. F. Postma
  • A. van Kleunen
  • W. Admiraal


Cadmium tolerance in field populations of the midge Chironomus riparius was studied by comparing the effects of chronic cadmium exposure on several life-history parameters using first generation, laboratory-reared animals. Differences between populations of C. riparius were therefore assumed to have a genetic basis. Field populations naturally exposed to metals were less sensitive to cadmium compared to unexposed populations, when larval development time and hatchability of the egg masses were measured. However, larval mortality still increased with cadmium exposure and no differences between exposed and unexposed populations were observed. Furthermore, life-history patterns differed between metal tolerant and nontolerant populations grown under control conditions. Metal tolerant populations were characterized by a high control mortality (50%) or an increased larval development time (with 30%). The results, therefore, indicated the presence of costs of tolerance, while a direct selection on certain life-history characteristics due to metal pollution was absent.


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  1. Antonovics J, Bradshaw AD, Turner RG (1971) Heavy metal tolerance in plants. Adv Ecol Res 7:1–85Google Scholar
  2. Bajraktari I, Savic G, Marinkovic D (1987a) The effect of heavy metals on the increase of genetic loads of Drosophila melanogaster viability. Acta Biol Med Exp 12:7–13Google Scholar
  3. Bajraktari I, Savic G, Marinkovic D, Hajrizi A (1987b) The influence of heavy metals on the duration of Drosophila melanogaster preadult development. Acta Biol Med Exp 12:57–66Google Scholar
  4. Chapco W, Jones SG, McConnell WB (1978) Correlations between chromosome segments and fitness in Drosophila melanogaster III. Differential genetic responses to zinc sulfate and selenocystine. Can J Genet Cytol 20:555–565Google Scholar
  5. Charlesworth B (1980) Evolution in age-structured populations. Cambridge University Press, CambridgeGoogle Scholar
  6. Comins HN (1977) The development of insecticide resistance in the presence of migration. J Theor Biol 64:177–197Google Scholar
  7. Croft BA, van de Baan HE (1988) Ecological and genetic factors influencing evolution of pesticide resistance in tetranychid and phytoseiid mites. Exp Appl Acarol 4:277–300Google Scholar
  8. Danks HV (1978) Some effects of photoperiod, temperature, and food on emergence in three species of chironomidae (Diptera). Can Entomol 110:289–290Google Scholar
  9. Davies BR (1976) The dispersal of Chironomidae larvae: A review. J Ent Soc Sth Afr 39:39–62Google Scholar
  10. Donker MH, Zonneveld C, van Straalen NM (1993) Early reproduction and increased reproductive allocation in metal adapted populations of the terrestrial isopod Porcellio scaber. Oecologia 96:316–323Google Scholar
  11. Falconer DS (1981) Introduction to quantitative genetics. Longman, New YorkGoogle Scholar
  12. Gadgil M, Bossert WH (1970) Life historical consequences of natural selection. The American Naturalist 104:1–24Google Scholar
  13. Hoffman ER, Fisher SW (1994) Comparison of a field and laboratory-derived population of Chironomus riparius (Diptera: Chironomidae): Biochemical and fitness evidence for population divergence. J Econ Entomol 87:318–325Google Scholar
  14. Kasai T, Watanabe T, Inoue K, Hasegawa T (1993) Purification and some properties of metal-binding proteins in housefly larvae (Musca domestica). Biosci Biotech Biochem 57:1873–1876Google Scholar
  15. Klerks PL, Weis JS (1987) Genetic adaptation to heavy metals in aquatic organisms: A review. Environ Pollut 45:173–205Google Scholar
  16. Kureck A (1979) Two circadian eclosion times in Chironomus thummi (Diptera), alternately selected with different temperatures. Oecologia 40:311–323Google Scholar
  17. — (1980) Circadian eclosion rhythm in Chironomus thummi: Ecological adjustment to different temperature levels and the role of temperature cycles. In: Murray DA (ed) Chironomidae: Ecology, systematics, cytology, and physiology. Pergamon Press, Oxford, pp. 73–78Google Scholar
  18. Macnair MR (1993) Transley review No. 49: The genetics of metal tolerance in vascular plants. New Phytol 124:541–559Google Scholar
  19. Magnusson J, Ramel C (1986) Genetic variation in the susceptibility to mercury and other metal compounds in Drosophila melanogaster. Teratogen Carcinogen Mutagen 6:289–305Google Scholar
  20. Maltby L (1991) Pollution as a probe of life-history adaptation in Asellus aquaticus (Isopoda). Oikos 61:11–18Google Scholar
  21. Maroni G, Wise J, Young JE, Otto E (1987) Metallothionein gene duplications and metal tolerance in natural populations of Drosophila melanogaster. Genetics 117:739–744Google Scholar
  22. McNeilly T (1968) Evolution in closely adjacent plant populations, III: Agrostis tenuis on a small copper mine. Heredity 23:99–108Google Scholar
  23. Mulvey M, Diamond SA (1991) Genetic factors and tolerance acquisition in populations exposed to metals and metalloids. In: Newman MC, McIntosh AW (eds) Metal Ecotoxicology: Concepts and applications. Lewis Publishers, Boca Raton, FLGoogle Scholar
  24. Nassar R (1979) Genetics of resistance of tetraethyllead. Aust J Biol Sci 32:127–132Google Scholar
  25. Otto E, Young JE, Maroni G (1986) Structure and expression of a tandem duplication of the Drosophila metallothionein gene. Proc Natn Acad Sci USA 83:6025–6029Google Scholar
  26. Pascoe D, Williams KA, Green DWJ (1989) Chronic toxicity of cadmium to Chironomus riparius—Effects on larval development and adult emergence. Hydrobiologia 175:109–115Google Scholar
  27. Pinder LCV (1978) A key to adult males of British Chironomidae. Freshwater Biological Association, Scientific Publ, No 37, Vols 1 and 2Google Scholar
  28. Posthuma L, Hogervost RF, van Straalen NM (1992) Adaptation to soil pollution by cadmium excretion in natural populations of Orchesella cincta (L.) (Collembola). Arch Environ Contam Toxicol 22:146–156Google Scholar
  29. Posthuma L, van Straalen NM (1993) Heavy-metal adaptation in terrestrial invertebrates: A review of occurrence, genetics, physiology, and ecological consequences. Comp Biochem Physiol 106C:11–38Google Scholar
  30. Posthuma L, Verweij RA, Widianarko B, Zonneveld C (1993) Life-history patterns in metal-adapted Collembola. Oikos 67:235–249Google Scholar
  31. Postma JF, Davids C (1995) Tolerance induction and life-cycle changes in cadmium exposed Chironomus riparius (Diptera) during consecutive generations. Ecotoxicol Environ Saf 30:195–202Google Scholar
  32. Postma JF, Kyed M, Admiraal W (in press) Site specific differentiation in metal tolerance in the midge Chironomus riparius (Diptera: Chironomidae). HydrobiologiaGoogle Scholar
  33. Postma JF, Mol S, Larsen H, Admiraal W (1995) Life-cycle changes and zinc shortage in cadmium tolerant midges, Chironomus riparius (Diptera), reared in the absence of cadmium. Environ Toxicol Chem 14:117–122Google Scholar
  34. Roesijadi G (1992) Metallothioneins in metal regulation and toxicity in aquatic animals. Aquat Toxicol 22:81–114CrossRefGoogle Scholar
  35. Roush RT, McKenzie JA (1987) Ecological genetics of insecticide and acaricide resistance. Ann Rev Entomol 32:361–380Google Scholar
  36. Seidman LA, Bergtrom G, Gingrich DJ, Remsen CC (1986) Accumulation of cadmium by the fourth instar larva of the fly Chironomus thummi. Tissue Cell 18:395–405Google Scholar
  37. Sibly RM, Calow P (1989) A life-cycle theory of responses to stress. Biol J Linnean Soc 37:101–116Google Scholar
  38. Sokal RR, Rohlf FJ (1981) Biometry, 2nd ed. W.H. Freeman and Co., NYGoogle Scholar
  39. Taylor CE, Georghiou GP (1979) Suppression of insecticide resistance by alteration of gene dominance and migration. J Econ Entomol 72:105–109Google Scholar
  40. Timmermans KR, van Hattum B, Kraak MHS, Davids C (1989) Trace metals in a littoral foodweb: Concentrations in organisms, sediment, and water. Sci Tot Environ 87/88:477–494Google Scholar
  41. Timmermans KR, Peeters W, Tonkes M (1992) Cadmium, zinc, lead, and copper in Chironomus riparius (Meigen) larvae (Diptera, Chironomidae): Uptake and effects. Hydrobiologia 241:119–134Google Scholar
  42. Tranvik L, Bengtsson G, Rundgren S (1993) Relative abundance and resistance traits of two Collembola species under metal stress. J Appl Ecol 30:43–52Google Scholar
  43. Trevors JT, Oddie KM, Belliveau BH (1985) Metal resistance in bacteria. FEMS Microbiol Rev 32:39–54Google Scholar
  44. van Hattum B, Timmermans KR, Govers HA (1991) Abiotic and biotic factors influencing in situ trace metal levels in macroinvertebrates in freshwater ecosystems. Environ Toxicol Chem 10:275–292Google Scholar
  45. Wentsel R, McIntosh A, Atchison G (1978) Evidence of resistance to metals in larvae of the midge Chironomus tentans in a metal contaminated lake. Bull Environ Contam Toxicol 20:451–455Google Scholar
  46. Yamamura M, Suzuki KT, Hatakeyama S, Kubota K (1983) Tolerance to cadmium and cadmium-binding proteins induced in the midge larva, Chironomus yoshimatsui (Diptera, Chironomidae). Comp Biochem Physiol 75C:21–24Google Scholar

Copyright information

© Springer-Verlag New York Inc 1995

Authors and Affiliations

  • J. F. Postma
    • 1
  • A. van Kleunen
    • 1
  • W. Admiraal
    • 1
  1. 1.Section of Aquatic EcotoxicologyUniversity of AmsterdamAmsterdamThe Netherlands

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