Human Genetics

, Volume 94, Issue 3, pp 259–264 | Cite as

A long range restriction map spanning the myxoid liposarcoma breakpoint in the q13-14 region of human chromosome 12

  • Anne Forus
  • Patrick F. J. Kools
  • Eric F. P. M. Schoenmakers
  • Wim J. M. Van de Ven
  • Ola Myklebost
Original Investigation
Received:
Revised:

Abstract

We have used pulsed-field gel electrophoresis to construct a long range restriction map of the myxoid liposarcoma (MLS) breakpoint region in 12q13-14. The CHOP/GADD153 gene, consistently translocated in myxoid liposarcomas, is located less than 55 kb from the putative oncogene GLI. We have used fluorescent in situ hybridization to orient the map with respect to the chromosome, and to show that GLI (and thus A2MR) is located proximal to the MLS breakpoint.

Keywords

Internal Medicine Metabolic Disease Long Range Range Restriction Human Chromosome 
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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Anne Forus
    • 1
  • Patrick F. J. Kools
    • 2
  • Eric F. P. M. Schoenmakers
    • 2
  • Wim J. M. Van de Ven
    • 2
  • Ola Myklebost
    • 1
  1. 1.Department of Tumor Biology, Institute for Cancer ResearchThe Norwegian Radium HospitalOsloNorway
  2. 2.Laboratory of Molecular Oncology, Center for Human GeneticsUniversity of LeuvenLeuvenBelgium

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