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Human Genetics

, Volume 94, Issue 6, pp 675–683 | Cite as

The immunoglobulin heavy chain and disease association: application to pemphigus vulgaris

  • William T. Gibson
  • Michael A. Walter
  • A. Razzaque Ahmed
  • Chester A. Alper
  • Diane W. Cox
Original Investigation

Abstract

Genes involved in the immune response are generally encoded from a complex cluster of gene segments. Studies of the association of diseases with such genes require well-defined genetic markers throughout the selected region. A set of 15 polymorphic loci that span 1500 kb of the immunoglobulin heavy chain (IGH) complex, 8 in the variable (VH) region and 7 in the constant (CH) region, were selected for the study of disease association. We present a protocol for the use of multiple immunoglobulin heavy chain (IGH) polymorphisms for general application in disease association studies. No microsatellite repeat markers are available for this region. To demonstrate the applicability of this approach, we have examined these IGH polymorphisms in families with individuals affected with pemphigus vulgaris (PV), an autoimmune dermatologie disease. Allele frequencies in 12 patients with PV were compared with those found in their spouses, and with those in a white Canadian control population. A significant difference was found between PV patients and both control groups for the presence of the VH gene VH3f-R4, and possibly for the absence of VH3f-R3, suggesting the possibility of susceptibility factors in these regions. Examination of the frequencies of the IGH region Cγ-haplotypes of PV patients indicated that, while the patients did not differ significantly from their spouses (χ2=1.79), both groups were found to differ significantly from the white Canadian control group (χ2=10.10), emphasizing the importance of matching the ethnic background of controls with that of the patient test group in disease association studies. Unexpectedly, two patients had large deletions of genes in the IGH constant region, which could play a role in the development of PV and require further investigation.

Keywords

Polymorphic Locus Gene Segment Large Deletion Pemphigus Disease Association 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • William T. Gibson
    • 1
    • 2
  • Michael A. Walter
    • 1
    • 2
  • A. Razzaque Ahmed
    • 4
  • Chester A. Alper
    • 3
  • Diane W. Cox
    • 1
    • 2
  1. 1.Research Institute, The Hospital for Sick ChildrenToronto
  2. 2.Departments of Paediatrics and Molecular and Medical GeneticsUniversity of TorontoTorontoCanada
  3. 3.The Center for Blood ResearchBostonUSA
  4. 4.Department of DermatologyBoston University, School of MedicineBostonUSA

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