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Cancer Immunology, Immunotherapy

, Volume 14, Issue 3, pp 196–201 | Cite as

Immunochemical characterization of a human high molecular weight — melanoma associated antigen identified with monoclonal antibodies

  • Barry S. Wilson
  • Giovanna Ruberto
  • Soldano Ferrone
Original Articles

Summary

Sodium dodecyl sulfate polyacrylamide gel analysis of a high molecular weight (HMW) human melanoma associated antigen (MAA) defined by murine monoclonal antibodies revealed a number of distinct polypeptides ranging from 80,000 up to 280,000 daltons, in addition to an extremely heterogeneous group of components distributed over a wide range in apparent molecular weight (300,000–700,000 daltons). The 280,000 dalton and the larger heterogeneous molecular weight material are glycosylated since they are labeled with 3H-sugars. The HMW-MAA is readily solubilized in the absence of detergents and the entire series of polypeptides fractionates together in the void volume of a Sephadex G200 column. Peptide maps of the various polypeptides of the HMW-MAA, generated by Staphylococcus aureus V-8 protease, are essentially the same except that some of the proteolytic fragments derived from the lower molecular weight polypeptides (80,000 daltons) are present in greater amounts than are similar fragments derived from the larger molecular weight polypeptides; the latter finding suggests that the complexity in molecular weight of the MAA may reflect combinations of several base subunits. Proteolytic cleavage of the HMW-MAA generates a number of peptides ranging in molecular weight from 77,000 daltons to less than 12,000 daltons, which still react with monoclonal antibodies and can distinguish monoclonal antibodies specific for different antigenic determinants of this MAA.

Keywords

Sodium Dodecyl Sulfate Apparent Molecular Weight Antigenic Determinant Large Molecular Weight G200 Column 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Barry S. Wilson
    • 1
  • Giovanna Ruberto
    • 2
  • Soldano Ferrone
    • 1
    • 2
  1. 1.Department of PathologyUniversity of Michigan Medical SchoolAnn Arbor
  2. 2.Departments of Pathology and SurgeryCollege of Physicians and Surgeons of Columbia UniversityNew YorkUSA

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