Involvement of abscisic acid in photosynthetic process in Hordeum vulgare L. during salinity stress

  • Losanka P. Popova
  • Zhivka G. Stoinova
  • Liliana T. Maslenkova
Article

Abstract

In Hordeum vulgare L. plants, NaCl stress imposed through the root medium for a period of 8 days decreased the rate of CO2 assimilation, the chlorophyll and protein leaf content, and the activity of ribulose-1,5-bisphosphate carboxylase. The activity of phosphoenolpyruvate carboxylase was twofold over the control. Pretreatment with abscisic acid (ABA) for 3 days before salinization diminished the inhibitory effect of NaCl on the rate of CO2 fixation. The leaf Na+ and Cl content decreased in ABA-pretreated plants. Both ABA and NaCl treatments led to an increase in the endogenous level of ABA in the plant leaves. Patterns of total proteins extracted from the leaves of control or ABA- and salt-treated plants were compared. Both ABA and NaCl induced marked quantitative and qualitative changes in the polypeptide profiles concerning mainly the proteins with approximately equal mobility. The results are discussed in terms of a possible role of ABA in increasing the salt tolerance when ABA is applied to the plants for a short period before exposure to salinity stress, thus improving the invulnerability to unfavorable conditions.

Abbreviations

RuBPC

ribulose-1,5-bisphosphate carboxylase

PSII

photosystem II

ABA

abscisic acid

PEPC

phosphoenolpyruvate carboxylase

DTTr

dithiothreitol

BSA

bovine serum albumin

ELISA

enzyme-linked immunosorbent assay

SDS

sodium dodecyl sulfate

PAGEr

polyacrylamide gel electrophoresis

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Copyright information

© Springer-Verlag New York Inc 1995

Authors and Affiliations

  • Losanka P. Popova
    • 1
  • Zhivka G. Stoinova
    • 1
  • Liliana T. Maslenkova
    • 1
  1. 1.Institute of Plant Physiology, Bulgarian Academy of SciencesSofiaBulgaria
  2. 2.Institute of Plant Physiology, Bulgarian Academy of SciencesSofiaBulgaria

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