Cell and Tissue Research

, Volume 227, Issue 3, pp 563–568 | Cite as

A cell line (HBL-100) established from human breast milk

  • Edwin V. Gaffney


A continuous cell line (HBL-100) was obtained from primary cultures of cells derived from an early lactation sample of human milk. There was no evidence of a breast lesion in the milk donor. Karyotype analysis showed that all metaphases contained human chromosomes including a large acrocentric marker chromosome. Both desmosomes and cytoplasmic tonofibrils were observed during early passage. HBL-100 cells exhibited several characteristics of transformation including the ability to form colonies in soft agar, an aneuploid chromosome complement, and continuous growth.

Key words

Milk Human Cell cultures Transformation 


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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Edwin V. Gaffney
    • 1
  1. 1.Microbiology Program, The Pennsylvania State UniversityUniversity ParkUSA

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