Organochlorine contaminant concentrations in eggs and their relationship to body size, and clutch characteristics of the female common snapping turtle (Chelydra serpentina serpentina) in lake Ontario, Canada

  • C. A. Bishop
  • G. P. Brown
  • R. J. Brooks
  • D. R. S. Lean
  • J. H. Carey


Statistical analyses were used to determine relationships between body size, clutch size and mass, and relative clutch mass and levels of organochlorine pesticides and seven polychlorinated biphenyl congeners in the eggs of adult female common snapping turtles (Chelydra serpentina serpentina). No significant correlations were found between body size (body mass, carapace length, carapace width, plastron length) and lipid normalized concentrations of p,p′-DDE, mirex, dieldrin, and polychlorinated biphenyl (PCB) congeners (IUPAC): #52, #105, #118, #138, #153, #180, #194, and the sum concentration of those congeners. Small sample size and clumping of data around the mode of the body size values prevented inferences of nonlinear relationships. It was concluded that body size and clutch characteristics are not strong or reliable predictors of the level of contaminants in snapping turtle eggs and that adjustment for those parameters would not reduce variation in contaminant levels among clutches. Other variables such as individual food preferences and/or foraging activities are more likely to cause variation in chemical concentrations among clutches of eggs within a population. In order to reduce interclutch variation in contaminant levels to 38.6–55.9% in snapping turtles, sample sizes of at least 15 clutches per site are recommended.


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  1. Alexander MM (1943) Food habits of the snapping turtle in Connecticut. J Wildl Mgmt 7:278–282Google Scholar
  2. Baumann PC, Whittle DM (1988) The status of selected organics in the Laurentian Great Lakes: An overview of DDT, PCBs, dioxins, furans, and aromatic hydrocarbons. Aquat Toxicol 11:241–257Google Scholar
  3. Ballschmiter K, Zell M (1980) Analysis of polychlorinated biphenyls (PCBs) by glass capillary chromatagraphy. Fresenius Z Anal Chem 302:20–31Google Scholar
  4. Bishop CA, Brooks RJ, Carey JH, Ng P, Norstrom RJ and Lean DRS (1991) The case for a cause-effect linkage between environmental contamination and development in eggs of the common snapping turtle (Chelydra serpentina) from Ontario, Canada. J Toxicol Environ Hlth 33:521–547Google Scholar
  5. Bishop CA (1990) The snapping turtle (Chelydra s. serpentina) as an indicator of organochlorine contamination in wetlands. MSc thesis, York University, North York, Ontario, CanadaGoogle Scholar
  6. Borgmann U, Whittle DM (1991) Contaminant concentration trends in Lake Ontario Lake Trout (Salvelinus namaycush): 1977 to 1988. J Great Lakes Res 17:368–381Google Scholar
  7. Congdon JD, Brettenbach Gl, Van Loben Sels RC, Tinkle DW (1987) Reproduction and nesting ecology of snapping turtles (Chelydra serpentina) in southeastern Michigan. Herpetologica 43:39–54Google Scholar
  8. Coulter M (1957) Predation by snapping turtles upon aquatic birds in Maine marshes. J Wild Man 20:17–21Google Scholar
  9. Denton GRW, Marsh GE, Heinsohn GE, Burdon-Jonew C (1980) The unusual metal status of the Dugong (Dugong dugon). Mar Biol 57:201–219Google Scholar
  10. Drescher HE, Harms U, Huschenbeth E (1977) Organochlorines and heavy metals in the harbour seal (Phoca vitulina) from the German North Sea Coast. Mar Biol 41:99–106Google Scholar
  11. Elliott JE, Whitehead P, Norstrom RJ, Sanderson JT, Hart LE, Cheng K, Bellward GD (1992) Monitoring biological effects of dioxin exposure in Great Blue Heron embryos. Annual meeting of Society of Environmental Toxicology and Chemistry, Abstract 581, p 133Google Scholar
  12. Galbraith DA (1986) Age estimates, survival, growth and maturity of female Cheyldra serpentina Linnaeus in Algonquin Provincial Park, Ontario. MSc. thesis, University of Guelph, Ontario, CanadaGoogle Scholar
  13. Gaskin DE, Stonefield KI, Suda P, Frank R (1979) Changes in mercury levels in Harbor porpoises from the Bay of Fundy, Canada and adjacent waters during 1960–1977. Arch Environ Contam Toxicol 8:733–762Google Scholar
  14. Gilbertson M, Elliott JE, Peakall DB (1987) Seabirds as indicators of marine pollution. In: Diamond AW and Filion FL (eds) The value of birds. Technical Publication No. 6. ICBP, pp 231–248Google Scholar
  15. Greichus YA, Greichus A, Emerick RJ (1973) Insecticides, polychlorinated biphenyls and mercury in wild cormorants, pelicans, their eggs, food and environment. Bull Environ Contam Toxicol 9:321–328Google Scholar
  16. Guiney PD, Peterson RE, Melancon MJ, Lech JJ (1979) Effects of egg and sperm maturation and spawning on the distribution and elimination of a polychlorinated biphenyl in rainbow trout (Salmo gairdneri). Toxicol Appl Pharm 47:261–272Google Scholar
  17. Harfenist A, Whitehead PE, Cretney WJ, Elliott JE (1993) Food chain sources of polychlorinated dioxins and furans to Great Blue Herons (Ardea herodia) foraging in the Fraser River Estuary, British Columbia. Technical Report Series No. 169. Canadian Wildlife ServiceGoogle Scholar
  18. Hebert CE, Glooschenko V, Haffner GD, Lazar R (1993) Organic contaminants in snapping turtle (Chelydra serpentina) populations from southern Ontario, Canada. Arch Environ Contam Toxicol 24:35–43Google Scholar
  19. Honda K, Tatsukawa R, Itano K, Nobuyuki M, Fujiyama T (1983) Heavy metal concentrations in muscle, liver and kidney tissue of striped dolphin (Stenella coeruleoalba) and their variations with body length, weight, age and sex. Agric Biol Chem 47:1219–1228Google Scholar
  20. Iverson JB, Smith GR (1993). Reproductive ecology of the painted turtle (Chrysemys picta) in Nebraska sandhills and across its range. Copeia:1–21Google Scholar
  21. Macdonald CR, Metcalfe CD (1989) A comparison of PCB congener distribution in two point source contaminated lakes and one uncontaminated lake in Ontario. Water Pollut Res J Can 24:23–46Google Scholar
  22. Metcalfe JL, Charlton MN (1990) Freshwater mussels as biomonitors for organic industrial contaminants and pesticides in the St. Lawrence River. Sci Total Environ 97/98:595–615Google Scholar
  23. Niimi AJ (1983) Biological and toxicological effects of environmental contaminants in fish and their eggs. Can J Fish Aquat Sci 40:306–312Google Scholar
  24. Norstrom RJ, Won HT (1985) Long-term preservation of egg and tissue homogenates for determination of organochlorine compounds freezing versus freeze drying. J Assoc Offic Anal Chem 68:129–135Google Scholar
  25. Norstrom RJ, Clark TP, Kearney JP, Gilman AP (1986a) Herring Gull requirements and body constituents in the Great Lakes. Ardea 74:1–23Google Scholar
  26. Norstrom RJ, Clark TP, Jeffrey DA, Won HT (1986b) Dynamics of organochlorine compounds in herring gulls (Larus argentatus) I. Distribution and clearance of [14C]DDE in free-living herring gulls (Larus argentatus). Environ Toxicol Chem 5:41–48Google Scholar
  27. Roudybush TE, Grau CR, Petersen MR, Ainsley DG, Hirsch KU, Gilman AP, Patten SM (1979) Yolk formation in some charadriiform birds. Condor 81:293–298Google Scholar
  28. Ryan JJ, Lau BPY, Hardy JA, Stone WB, O'Keefe P, Gierthy JF (1986) 2,3,7,8-Tetrachlorodibenzo-p-dioxin and related dioxins and furans in snapping turtle (Chelydra serpentina) tissues. Chemosphere 15:537–548Google Scholar
  29. Scott DP, Armstrong FAJ (1972) Mercury concentration in relation to size in several species of freshwater fishes from Manitoba and northwestern Ontario. J Fish Res Board Can 29:1685–1690Google Scholar
  30. Semkin R, McLarty AW, Craig D (1976) A water quality study of Cootes Paradise. Ontario Ministry of the Environment, Water Resources Assessment Technical Support Section, Toronto, Ontario, Canada, p 15Google Scholar
  31. Sokal RR, Rohlf FJ (1981) Biometry, 2nd ed. WH Freeman, NY, pp 607, 618–642Google Scholar
  32. Spellerberg IF (1982) Biology of reptiles. Blackie and Son Ltd, London, UK, p 102Google Scholar
  33. Struger J, Elliott JE, Bishop CA, Weseloh DV, Norstrom RJ, Simon M, Ng P (1993) Environmental contamination in eggs of the common snapping turtle (Chelydra serpentina serpentina) from the Great Lakes-St. Lawrence River basin in Ontario, Canada (1981, 1984). J Great Lakes Res 19(4):681–694Google Scholar
  34. Suns K, Hitchin G, Toner D (1991) Spatial and temporal trends of organochlorine contaminants in spottail shiners (Notropis hudsonius) from the Great Lakes and their connecting channels (1975–1988). Ontario Ministry of Environment, Water Resources Branch, Ontario, Canada, p 85Google Scholar
  35. Tanabe S, Subramanian AN, Hidaka H, Tatsukawa R (1986) Transfer rates and pattern of PCB isomers and congeners and p,p′-DDE from mother to egg in adelie penguin (Pygoscelis adeliae). Chemosphere 15:341–351Google Scholar
  36. Weseloh DV, Mineau P, Hallett DJ (1979) Organochlorine contaminants and trends in reproduction in Great Lakes herring gulls, 1974–1978. In: Transactions of the 44th North American Wildlife and Natural Resources Conference. Wildlife Mgmt. Institute, pp 543–557Google Scholar
  37. White JB, Murphy GG (1973) The reproductive cycle and sexual dimorphism of the common snapping turtle, Chelydra serpentina serpentina. Herpetologica 29:240–246Google Scholar
  38. Whittle DM, Fitzsimons JD (1983) The influence of the Niagara River on contaminant burdens of Lake Ontario biota. J Great Lakes Res 9(2):295–302Google Scholar
  39. Youngs WD, Gutenmann WH, Lisk DJ (1972) Residues of DDT in lake trout as a function of age. Environ Sci Technol 6(5):451–452Google Scholar

Copyright information

© Springer-Verlag New York Inc 1994

Authors and Affiliations

  • C. A. Bishop
    • 1
  • G. P. Brown
    • 1
  • R. J. Brooks
    • 1
  • D. R. S. Lean
    • 1
  • J. H. Carey
    • 1
  1. 1.Environment CanadaCanadian Wildlife Service (Ontario)BurlingtonCanada

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