Planta

, Volume 196, Issue 3, pp 425–429 | Cite as

Biological activity of galactoglucomannan-derived oligosaccharides

  • D. Lišková
  • O. Auxtová
  • D. Kákoniová
  • M. Kubačková
  • Š. Karácsonyi
  • L. Bilisics
Article
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Accepted:

Abstract

A mixture of galactoglucomannan-derived oligosaccharides (GGMOs), degree of polymerization 4–8, (≈1.2 μM and ≈12 μM) stimulated the viability of spruce [Picea abies (L.) Karst] embryos predominately on media supplemented with indole-3-acetic acid: zeatin (0.01∶1, 1∶0.01 mg · 1-1), at pH 5.O. Their effects on the development and morphogenesis of embryos were dependent on the culture conditions used. These GGMOs also improved the viability of spruce protoplasts when applied at the same concentrations in combination with 1-naphthaleneacetic acid, and to a lesser extent with 2,4-dichlorophenoxyacetic acid at pH 3.8. Viability was also maintained in the presence of GGMOs when the growth hormones were absent; however, the efficiency of protoplast division was low.

Key words

Callus Galactoglucomannan-derived oligosaccharides Morphogenesis Picea Protoplast Viability Zygotic embryo 

Abbreviations

2,4-D

2,4-dichlorophenoxyacetic acid

NAA

1-naphthaleneacetic acid

GGMOs

galactoglucomannan-derived oligosaccharides

IAA

indole-3-acetic acid

zeatin

6-[4-hydroxy-3-methyl-but-2-enylamino]purine

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • D. Lišková
    • 1
  • O. Auxtová
    • 1
  • D. Kákoniová
    • 1
  • M. Kubačková
    • 1
  • Š. Karácsonyi
    • 1
  • L. Bilisics
    • 1
  1. 1.Institute of Chemistry, Slovak Academy of SciencesBratislavaSlovak Republic

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