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Planta

, Volume 192, Issue 4, pp 557–566 | Cite as

Use of two-dimensional protein-pattern analysis for the characterization of Arabidopsis thaliana mutants

  • Véronique Santoni
  • Catherine Bellini
  • Michel Caboche
Article

Abstract

Total proteins extracted from wild-type plants of Arabidopsis thaliana Heyhn, an etiolated mutant, a de-etiolated mutant and a mutant affected in cotyledon morphology, were analyzed by two-dimensional gel electrophoresis. Computer analysis of two-dimensional gels allowed the characterization of the mutants by a set of proteins showing a differential expression when compared with the wild-type plant grown under the same conditions. The overlap between comparisons of the diferent mutants with the wild-type allowed the identification of groups of polypeptides which, since their expression is altered in several mutants, might be involved in certain physiological functions. For example, this approach showed a possible involvement of actin in the elongation process. The simultaneous analysis of the two-dimensional protein patterns of different mutants seems, therefore, to be a promising approach to characterize proteins involved in various physiological functions.

Key words

Arabidopsis mutants Protein characterization Two-dimensional gel electrophoresis 

Abbreviations

2-D

two-dimensional

IEF

isoelectrofocusing

Mr

relative molecular mass

E17.1

E17 mutant grown in light

E22.1

E22 mutant grown in light

E44.d

E44 mutant grown in darkness

WT.d

wild-type plants grown in darkness

WT.1

Wild-type plants grown in light

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Véronique Santoni
    • 1
  • Catherine Bellini
    • 1
  • Michel Caboche
    • 1
  1. 1.I.N.R.A., Laboratoire de Biologie CellulaireVersailles CedexFrance

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