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Medical Microbiology and Immunology

, Volume 178, Issue 2, pp 121–125 | Cite as

Isolation and interspecies-transfer of a plasmid from Pasteurella multocida encoding for streptomycin resistance

  • St. Schwarz
  • U. Spies
  • F. Schäfer
  • H. Blobel
Article

Abstract

A plasmid encoding streptomycin-resistance could be detected in 13 of 32 Pasteurella multocida-cultures isolated from cattle and swine. The plasmid of these cultures proved to be similar upon Southern blot hybridization. It could be transformed into Escherichia coli 490A, where it also expressed streptomycin resistance.

Keywords

Escherichia Coli Streptomycin Southern Blot Blot Hybridization Southern Blot Hybridization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Baldwin DE, Marshall RG, Wessman GE (1968) Pasteurellae in bovine respiratory disease. J Am Vet Med Assoc 152:824–828Google Scholar
  2. 2.
    Barry AL, Thornsberry C Susceptibility tests: diffusion test procedures. In: Lennette EH, Balows A, Hausler WH Jr, Shadomy HJ (eds) Manual of clinical microbiology, 4th ed. American Society for Microbiology, Washington, DC pp 978–987Google Scholar
  3. 3.
    Berman SM, Hirsh DC (1978) Partial characterization of R-plasmids from Pasteurella multocida isolated from turkeys. Antimicrob Agents Chemother 14 (3):348–352Google Scholar
  4. 4.
    Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucl Ac Res 7 (6): 1513–1523Google Scholar
  5. 5.
    Carter GR (1981) Pasteurella. In: Blobel H, Schließer Th (Hrsg) Handbuch der bakteriellen Infektionen bei Tieren, Bd III. Gustav Fischer Verlag, Stuttgart New YorkGoogle Scholar
  6. 6.
    Carter GR (1984) Pasteurellaceae. In: Buchanan RE, Gibbons NE (eds) Bergey's manual of systematic bacteriology, vol 1, 8th ed. The Williams and Wilkins Company, Baltimore, pp 552–558Google Scholar
  7. 7.
    Carter GR (1955) Studies on Pasteurella multocida: I. A hemagglutination test for the identification of serological type. Am J Vet Res 16:481–484Google Scholar
  8. 8.
    Carter GR (1973) Identification of type D strains of Pasteurella multicodia with acriflavine. Am J Vet Res 34:293–294Google Scholar
  9. 9.
    Chang YF, Renshaw HW, Young R (1987) Pneumonic pasteurellosis: Examination of typable and untypable Pasteurella haemolytica strains for leukotoxin production, plasmid content and antimicrobial susceptibility. Am J Vet Res 48 (3):378–384Google Scholar
  10. 10.
    Cohen SN, Chang ACY, Hsu L (1972) Nonchromosomal antibiotic resistance in bacteria: Genetic transformation of E. coli by R-factor DNA. Proc Nat Acad Sci 69 (8):2110–2114Google Scholar
  11. 11.
    Collier JR (1968) Pasteurellae in bovine respiratory disease. J Am Vet Med Assoc 152:824–828Google Scholar
  12. 12.
    Farrar EW Jr. (1983) Molecular analysis of plasmids in epidemiologic investigation. J Infect Dis 148 (1):1–6Google Scholar
  13. 13.
    Gene Screen Plus (1985) Catalog No NEF-976. Protocols for Electrophoretic and Capillary Transfer of DNA and RNA, DNA and RNA Hybridization, and DNA and RNA Rehybridization. Du Pont Company, Boston, USAGoogle Scholar
  14. 14.
    Gyles CL, Thoen CO (1986) Pathogenesis of bacterial infections in animals. Iowa University PressGoogle Scholar
  15. 15.
    Haghour R, Hellmann E, Schmidt J (1987) Plasmids and resistance to 9 chemotherapeutic agents of Pasteurella multocida and Pasteurella haemolytica. Epidemiological Aspects. J Vet Med B 34:509–518Google Scholar
  16. 16.
    Hirsh DC, Lori MD, Rhoades KR (1985) Resistance plasmids of Pasteurella multocida isolated from turkeys. Am J Vet Res 46 (7): 1490–1493Google Scholar
  17. 17.
    Knippers R (1985) Molekulare Genetik, 4. Aufl. Georg Thieme Verlag, Stuttgart New YorkGoogle Scholar
  18. 18.
    Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbour Laboratory, Cold Spring Harbour, NYGoogle Scholar
  19. 19.
    Mayer H, Grosschedl R, Schütte H, Hobom G (1981) ClaI, a new restriction endonuclease from Caryophanon latum L. Nucl Ac Res 9:4833–4845Google Scholar
  20. 20.
    O'Brien TF, Ross DG, Guzman MA, Medeiros AA, Hedges RW, Botstein D (1980) Dissemination of an antibiotic resistance plasmid in hospital patient flora. Antimicrob Agents Chemother 17:537–543Google Scholar
  21. 21.
    Rolle M, Mayr A (1984) Medizinische Mikrobiologie, Infektions- und Seuchenlehre, 5. Aufl., EnkeVerlag, StuttgartGoogle Scholar
  22. 22.
    Schwarz St, Spies U, Reitz B, Seyfert H.-M., Lämmler CH, Blobel H (1989) Detection and interspecies-transformation of a β-lactamase-encoding plasmid from Pasteurella haemolytica. Zentralbl Bakteriol Mikrobiol Hyg [A] (in press)Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • St. Schwarz
    • 1
  • U. Spies
    • 2
  • F. Schäfer
    • 3
  • H. Blobel
    • 1
  1. 1.Institut für Bakteriologie und Immunologie der Universität GiessenGiessenFederal Republic of Germany
  2. 2.Institut für Virologie der Universität GiessenGiessenFederal Republic of Germany
  3. 3.Staatl. Institut für Gesundheit und Umwelt, Abt. VeterinärmedizinSaarbrückenFederal Republic of Germany

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