, Volume 2, Issue 2, pp 89–96 | Cite as

Ectomycorrhizal syntheses with Picea abies and three fungal species: a case study on the use of an in vitro technique to identify naturally occurring ectomycorrhizae

  • I. Brunner
  • R. Amiet
  • M. Zollinger
  • S. Egli
Original Papers


Ectomycorrhizal syntheses between Picea abies and the fungal associates Scleroderma citrinum, Boletus luridus, and Tricholoma vaccinum were carried out using Melin's Erlenmeyer flask technique. The symbioses of S. citrinum were characterized by a mantle composed of an outer prosenchymatous and an inner synenchymatous layer. The mantles of B. luridus and T. vaccinum were solely prosenchymatous. Rhizomorphs were produced in all treatments, but only in association with S. citrinum were they differentiated with additional, enlarged hyphae. All synthesized ectomycorrhizae were white or whitish to light orange and greyishorange. On large-scale root sampling in two differing Picea abies forests in Switzerland, nine out of a total of 22 morphological types of ectomycorrhizae were white or yellow in colour and were, therefore, comparable with the synthesized ectomycorrhizae. These nine natural types generally had distinct mantle features (irregular synenchyma, gelatinous matrix, cystides, thick-walled hyphae), but mostly lacked clamp connections. Synthesized ectomycorrhizae, on the other hand, lacked distinct mantle characteristics and always had clamp connections. Natural and synthesized white or yellow ectomycorrhizae did not coincide morphologically and thus identification of the fungal partners of natural symbioses by means of in vitro-synthesis with potential ectomycorrhizal fungi was not possible in the present study.

Key words

Ectomycorrhizal synthesis Erlenmeyer flask technique Picea abies Large-scale root sampling Morphological types 


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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • I. Brunner
    • 1
  • R. Amiet
    • 1
  • M. Zollinger
    • 1
  • S. Egli
    • 1
  1. 1.Swiss Federal Institute for Forest, Snow and Landscape Research (WSL)BirmensdorfSwitzerland

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