, Volume 5, Issue 2, pp 89–97

Formation and growth of the ectomycorrhiza of Cantharellus cibarius

  • Eric Danell
Original Paper


New data on the physiology of Cantharellus cibarius mycorrhiza formation has resulted in a new aseptic routine method for in vitro formation. The advances are short formation time, healthy plants and reliable colonization. A high glucose demand and a good gas exchange with additional carbon dioxide are important factors in the mycorrhiza formation. Mycorrhiza was observed after 8 weeks, but strong colonization occurred after 10–12 weeks, when mycorrhiza was established to the depth of 5 cm. A C. cibarius strain connected to Picea abies in nature successfully colonized Pinus sylvestris in vitro, but not Betula pendula. Mycorrhizal plants have been successfully transferred to unsterile environments in greenhouses. The mycorrhizae continued to colonize new roots and the unsterile peat soil for 10 months. However, C. cibarius mycorrhiza is highly sensitive to flooding. With PCR and RFLP, fruit bodies, isolated mycelia and artificially formed mycorrhizae have been compared to prove that C. cibarius was used. Climatic changes did not induce primordia formation but factors behind fruit body formation are discussed.

Key words

Mushroom Polymerase chain reaction Mycorrhiza 


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  1. Agerer R (1985) Zur Ökologie der Mykorrhizapilze. Bibl Mycol 97:1–160Google Scholar
  2. Aschan-Åberg K (1958) The production of fruit bodies in Collybia velutipes. II. Further studies on the influence of different culture conditions. Physiol Plant 11:312–328Google Scholar
  3. Chevalier G, Frochot H (1981) Truffle production from artificially mycorrhizal plants: first results. In: Fortin JA (ed) Abstracts of the 5th North American Conference on Mycorrhizae, 16–21 August, Quebec, Canada, p 35Google Scholar
  4. Danell E, Fries N (1990) Methods for isolation of Cantharellus species, and the synthesis of ectomycorrhizae with Picea abies. Mycotaxon 38:141–148Google Scholar
  5. Danell E, Alström S, Ternström A (1993) Pseudomonas fluorescens in association with fruit bodies of the ectomycorrhizal mushroom Cantharellus cibarius. Mycol Res 97:1148–1152Google Scholar
  6. Danielson R (1984) Ectomycorrhizal associations in jack pine stands in northeastern Alberta. Can J Bot 62:932–939Google Scholar
  7. Debaud JC, Gay G (1987) In vitro fruiting under controlled conditions of the ectomycorrhizal fungus Hebeloma cylindrosporum associated with Pinus pinaster. New Phytol 105:429–435Google Scholar
  8. Dighton J, Mason PA (1985) Mycorrhizal dynamics during forest tree development. In: Moore D, Casselton LA, Wood DA, Frankland JC (eds) Developmental biology of higher fungi. Cambridge University Press, Cambridge, UK, pp 117–139Google Scholar
  9. Fleming LV (1984) Effects of soil trenching and coring on the formation of ectomycorrhizas on birch seedlings grown around mature trees. New Phytol 98:143–153Google Scholar
  10. Fortin JA, Piché Y, Lalonde M (1980) Technique for the observation of early morphological changes during ectomycorrhiza formation. Can J Bot 58:361–365Google Scholar
  11. Fries N (1979) Germination of spores of Cantharellus cibarius. Mycologia 71:216–219Google Scholar
  12. Fries N (1981) Recognition reactions between basidiospores and hyphae in Leccinum. Trans Br Mycol Soc 77:9–14Google Scholar
  13. Fries N (1987) The third benefactors lecture: ecological and evolutionary aspects of spore germination in the higher basidiomycetes. Trans Br Mycol Soc 88:1–7Google Scholar
  14. Froidevaux L (1975) Identification of some Douglas fir mycorrhizae. Eur J For Pathol 5:212–216Google Scholar
  15. Garbaye J, Duponnois R, Wahl JL (1990) The bacteria associated with Laccaria laccata ectomycorrhizas or sporocarps: effect on symbiosis establishment on Douglas fir. Symbiosis 9:267–273Google Scholar
  16. Garbaye J, Churin J-L, Duponnois R (1992) Effects of substrate sterilization, fungicide treatment, and mycorrhization helper bacteria on ectomycorrhizal fonnation of pedunculate oak (Quercus robur) inoculated with Laccaria laccata in two peat bare-root nurseries. Biol Fertil Soils 13:55–57Google Scholar
  17. Gardes M, White TJ, Fortin JA, Bruns TD, Taylor JW (1991) Identification of indigenous and introduced symbiotic fungi in ectomycorrhizae by amplification of nuclear and mitochondrial ribosomal DNA. Can J Bot 69:180–190Google Scholar
  18. Godbout C, Fortin A (1990) Cultural control of basidiome formation in Laccaria bicolor with container-grown white pine seedlings. Mycol Res 94:1051–1058Google Scholar
  19. Godbout C, Fortin A (1992) Effects of nitrogen fertilization and photoperiod on basidiome formation of Laccaria bicolor associated with container-grown jack pine seedlings. Can J Bot 70:181–185Google Scholar
  20. Harley JL, Smith SE (1983) Mycorrhizal symbiosis. Academic Press, LondonGoogle Scholar
  21. Hedger J (1986) Suillus luteus on the equator. Bull Br Mycol Soc 20:53–54Google Scholar
  22. Henrion B, Le Tacon F, Martin F (1992) Rapid identification of genetic variation of ectomycorrhizal fungi by amplification of ribosomal RNA genes. New Phytol 122:289–298Google Scholar
  23. Henrion B, Chevalier G, Martin F (1994) Typing truffle species by PCR amplification of the ribosomal DNA spacers. Mycol Res 98:37–43Google Scholar
  24. Ingestad T (1979) Mineral nutrient requirements of Pinus sylvestris and Picea abies seedlings. Physiol Plant 45:373–380Google Scholar
  25. Itävaara M, Willberg H (1988) Establishment of a Cantharellus cibarius culture collection in Finland. Karstenia 28:34Google Scholar
  26. Jentschke G, Godbold DL, Hütterman A (1991) Culture of mycorrhizal tree seedlings under controlled conditions: effects of nitrogen and aluminium. Physiol Plant 81:408–416Google Scholar
  27. Kähr M, Arveby AS (1986) A method for establishing ectomycorrhiza on conifer seedlings in steady-state conditions of nutrition. Physiol Plant 67:333–339Google Scholar
  28. Kälin I, Ayer F (1983) Sporenabwurf und Fruchtkoerperentwick-lung des goldstieligen Pfifferlings (Cantharellus lutescens) im Zusammenhang mit Klimafaktoren. Mycol Helv 1:67–88Google Scholar
  29. Last FT, Fleming LV (1985) Factors affecting the occurrence of fruitbodies of fungi forming sheathing (ecto-) mycorrhizas with roots of trees. Proc Indian Acad Sci Plant Sci 94:111–127Google Scholar
  30. Last FT, Mason PA, Pelham J, Ingleby K (1984) Fruitbody production by sheathing mycorrhizal fungi: effects of “host” genotypes and propagating soils. For Ecol Manage 9:221–227Google Scholar
  31. Magnusson T (1992) Temporal and spatial variation of the soil atmosphere in forest soils of northern Sweden. PhD thesis, Swedish University of Agricultural Sciences, Umeå, SwedenGoogle Scholar
  32. McLaughlin DJ (1970) Environmental control of fruitbody development in Boletus rubinellus in axenic culture. Mycologia 62:307–331Google Scholar
  33. Melin E (1922) Untersuchungen über die Larix Mycorrhiza.I.Synthese der Mykorrhiza in Reinkulturen. Svensk Bot Tidskr 15:192–193Google Scholar
  34. Melin E (1925) Untersuchungen über die Bedeutung der Baummykorrhiza. Fischer, JenaGoogle Scholar
  35. Modess O (1941) Zur Kenntnis der Mykorrhizabildner von Kiefer und Fichte. Symb Bot Upsal 5:1–149Google Scholar
  36. Molina R, Palmer JG (1982) Isolation, maintenance, and pure culture manipulation of ectomycorrhizal fungi. In: Schenck NC (ed) Methods and principles of mycorrhizal research. American Phytopathological Society, St Paul, pp 115–129Google Scholar
  37. Moore L, Jansen A, Van Griensven L (1989) Pure culture synthesis of ectomycorrhizas with Cantharellus cibarius. Acta Bot Neerl 38:273–278Google Scholar
  38. Norvell L (1992) Studying the effects of harvesting on chanterelle productivity in Oregon's Mt. Hood National Forest. In: Gues N de (ed) Wild mushroom harvesting discussion session minutes, Victoria, March 3, 1992. Ministry of Forests, Victoria, pp9–15Google Scholar
  39. Nylund J-E, Wallander H (1989) Effects of ectomycorrhiza on host growth and carbon balance in a semi-hydroponic cultivation system. New Phytol 112:389–398Google Scholar
  40. Romell L-G (1938) A trenching experiment in spruce forest and its bearing on problems on mycotrophy. Svensk Bot Tidskr 32:89–99Google Scholar
  41. Schouten SP, Waandrager MH (1979) Problems in obtaining pure cultures of Cantharellus cibarius. Mushroom Sci 10:885–890Google Scholar
  42. Stenström E (1991) The effects of flooding on the formation of ectomycorrhizae in Pinus sylvestris seedlings. Plant Soil 131:247–250Google Scholar
  43. Straatsma G, Bruinsma J (1986) Carboxylated metabolic intermediates as nutritional factors in vegetative growth of the mycorrhizal mushroom Cantharellus cibarius Fr. J Plant Physiol 125:377–381Google Scholar
  44. Straatsma G, Van Griensven LJLD (1986) Growth requirements of mycelial cultures of the mycorrhizal mushroom Cantharellus cibarius. Trans Br Mycol Soc 87:135–141Google Scholar
  45. Straatsma G, Konings RNH, Van Griensven LJLD (1985) A strain collection of the mycorrhizal mushroom Cantharellus cibarius obtained by germination of spores and culture of fruit body tissue. Trans Br Mycol Soc 85:689–697Google Scholar
  46. Straatsma G, Van Griensven LJLD, Bruinsma J (1986) Root influence on in vitro growth of hyphae of the mycorrhizal mushroom Cantharellus cibarius replaced by carbon dioxide. Physiol Plant 67:521–528Google Scholar
  47. Trappe J (1962) Fungus associates of ectotrophic mycorrhizae. Bot Rev 28:538–606Google Scholar
  48. Unestam T (1991) Water repellency, mat formation, and leaf-stimulated growth of some ectomycorrhizal fungi. Mycorrhiza 1:13–20Google Scholar
  49. Unestam T, Stenström E (1989) A method for observing and manipulating roots and root associated fungi on plants growing in nonsterile substrates. Scand J For Res 4:51–58Google Scholar
  50. Wardlaw IF (1990) Transley review no.27. The control of carbon partitioning in plants. New Phytol 116:341–381Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Eric Danell
    • 2
  1. 1.Department of Forest ScienceOregon State UniversityCorvallisUSA
  2. 2.Department of Physiology BotanyUniversity of UppsalaUppsalaSweden

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