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Planta

, Volume 193, Issue 3, pp 372–376 | Cite as

Ultraviolet light and ozone stimulate accumulation of salicylic acid, pathogenesis-related proteins and virus resistance in tobacco

  • Nasser Yalpani
  • Alexander J. Enyedi
  • Jose León
  • Ilya Raskin
Article

Abstract

In tobacco (Nicotiana tabacum L. cv. Xanthinc), salicylic acid (SA) levels increase in leaves inoculated by necrotizing pathogens and in healthy leaves located above the inoculated site. Systemic SA increase may trigger disease resistance and synthesis of pathogenesis-related proteins (PR proteins). Here we report that ultraviolet (UV)-C light or ozone induced biochemical responses similar to those induced by necrotizing pathogens. Exposure of leaves to UV-C light or ozone resulted in a transient ninefold increase in SA compared to controls. In addition, in UV-light-irradiated plants, SA increased nearly fourfold to 0.77 μg·g−1 fresh weight in leaves that were shielded from UV light. Increased SA levels were accompanied by accumulation of an SA conjugate and by an increase in the activity of benzoic acid 2-hydroxylase which catalyzes SA biosynthesis. In irradiated and in unirradiated leaves of plants treated with UV light, as well as in plants fumigated with ozone, PR proteins 1a and 1b accumulated. This was paralleled by the appearance of induced resistance to a subsequent challenge with tobacco mosaic virus. The results suggest that UV light, ozone fumigation and tobacco mosaic virus can activate a common signal-transduction pathway that leads to SA and PR-protein accumulation and increased disease resistance.

Key words

Disease resistance Nicotiana (disease resistance) Ozone Pathogenesis related protein Salicylic acid Ultraviolet irradiation 

Abbreviations

PR protein

pathogenesis-related protein

SA

salicylic acid

TMV

tobacco mosaic virus

UV

ultraviolet

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Nasser Yalpani
    • 1
  • Alexander J. Enyedi
    • 1
  • Jose León
    • 1
  • Ilya Raskin
    • 1
  1. 1.AgBiotech Center, Cook College, Rutgers UniversityNew BrunswickUSA

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