Planta

, Volume 194, Issue 1, pp 143–148

Elicitor-induced formation of free and cell-wall-bound stilbenes in cell-suspension cultures of Scots pine (Pinus sylvestris L.)

  • Bernd Markus Lange
  • Monika Trost
  • Werner Heller
  • Christian Langebartels
  • Heinrich SandermannJr.
Article

DOI: 10.1007/BF00201045

Cite this article as:
Lange, B.M., Trost, M., Heller, W. et al. Planta (1994) 194: 143. doi:10.1007/BF00201045

Abstract

Treatment of Pinus sylvestris L. cell-suspension cultures with an elicitor preparation from the pine needle pathogen Lophodermium seditiosum, resulted in a severalhundredto thousandfold accumulation of the stilbenes pinosylvin and pinosylvin 3-O-methyl ether in methanolic cell extracts. There was a simultaneous induction of the biosynthetic enzymes phenylalanine ammonia-lyase (E.C. 4.3.1.5.) and stilbene synthase (pinosylvin-forming, E.C. 2.3.1.146). For the first time, an incorporation of stilbenes into the cell wall fraction as well as stilbene excretion into the extracellular space was demonstrated in addition to intracellular accumulation.

Key words

Elicitor Enzyme induction Lophodermium Pinus Stilbene metabolism 

Abbreviations

PAL

phenylalanine ammonia-lyase

PS

pinosylvin

PSM

pinosylvin 3-O-methyl ether

STS

stilbene synthase (pinosylvin-forming)

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Bernd Markus Lange
    • 1
  • Monika Trost
    • 1
  • Werner Heller
    • 1
  • Christian Langebartels
    • 1
  • Heinrich SandermannJr.
    • 1
  1. 1.GSF — Forschungszentrum für Umwelt und Gesundheit GmbH, Institut für Biochemische PflanzenpathologieOberschleissheimGermany

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