Elicitor-induced formation of free and cell-wall-bound stilbenes in cell-suspension cultures of Scots pine (Pinus sylvestris L.)
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- Lange, B.M., Trost, M., Heller, W. et al. Planta (1994) 194: 143. doi:10.1007/BF00201045
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Treatment of Pinus sylvestris L. cell-suspension cultures with an elicitor preparation from the pine needle pathogen Lophodermium seditiosum, resulted in a severalhundredto thousandfold accumulation of the stilbenes pinosylvin and pinosylvin 3-O-methyl ether in methanolic cell extracts. There was a simultaneous induction of the biosynthetic enzymes phenylalanine ammonia-lyase (E.C. 126.96.36.199.) and stilbene synthase (pinosylvin-forming, E.C. 188.8.131.52). For the first time, an incorporation of stilbenes into the cell wall fraction as well as stilbene excretion into the extracellular space was demonstrated in addition to intracellular accumulation.
Key wordsElicitor Enzyme induction Lophodermium Pinus Stilbene metabolism
pinosylvin 3-O-methyl ether
stilbene synthase (pinosylvin-forming)