Cancer Immunology, Immunotherapy

, Volume 3, Issue 2, pp 131–136 | Cite as

Cell mediated cytotoxicity to human melanoma cell lines: Nonspecific active in vitro augmentation

  • A. S. Coates
  • M. Crawford
Original Papers
Accepted:
  • 11 Downloads

Summary

An in vitro system to measure nonspecific augmentation of cytotoxicity toward human melanoma cell lines is described. Mononuclear cells separated from peripheral blood on isopaque-ficoll gradients were cultured with the mitogens phytohemagglutinin, concanavalin-A, and pokeweed, with Bacillus Calmette-Guerin (BCG), or with allogeneic lymphocytes. In each case, marked augmentation of cytotoxicity toward both melanoma cell lines and a normal fibroblast line was observed. Populations enriched for T cells by E-rosetting or nylon fiber filtration showed unimpaired responsiveness to BCG, while populations depleted of T cells by E-rosetting showed neither “natural” cytotoxicity nor augmentation of cytotoxicity by BCG.

Keywords

Melanoma Cancer Research Nylon Mononuclear Cell Melanoma Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Baldwin, R. W., Pimm, M. V.: BCG suppression of pulmonary metastases from primary rat hepatoma. Brit. J. Cancer 30, 473 (1974)Google Scholar
  2. Bevan, M. J., Cohn, M.: Cytotoxic effects of antigen- and mitogen-induced T cells on various targets. J. Immunol. 114, 559 (1975)Google Scholar
  3. Boyum, A.: Separation of leucocytes from blood and bone marrow. Scand. J. Clin. Lab. Invest. 21, Suppl. 97 (1968)Google Scholar
  4. Burton, R., Thompson, J., Warner, N. L.: In vitro induction of tumour-specific immunity. I. Development of optimal conditions for induction and assay of cytotoxic lymphocytes. J. Immunol. Methods 8, 133 (1975)Google Scholar
  5. Cerottini, J.-C., Engess, H. D., Macdonald, H. R., Brunner, K. T.: Generation of cytotoxic T lymphocytes in vitro. I. Response of normal and immune mouse spleen cells in mixed lymphocyte cultures. J. Exp. Med. 140, 704 (1974)Google Scholar
  6. Coates, A.: Current status of immunotherapy. Drugs 8, 405 (1974)Google Scholar
  7. Coates, A., Crawford, M.: Growth of human melanoma cell lines in nude mice: suppression by human T-lymphocytes from the tumor donor. J. Natl. Cancer Inst. 59, 1325 (1977)Google Scholar
  8. Dunkley, M., Miller, R. G., Shortman, K.: A modified 51Cr release assay for cytotoxic lymphocytes. J. Immunol. Methods 6, 39 (1974)Google Scholar
  9. Eilber, F. R., Morton, D. L., Holmes, E. C., Sparks, F. C., Ramming, K. P.: Adjuvant immunotherapy with BCG in treatment of regional lymph-node metastases from malignant melanoma. New Eng. J. Med. 294, 237 (1976)Google Scholar
  10. Greaves, M. F., Brown, G.: Purification of human T and B lymphocytes. J. Immunol. 112, 420 (1974)Google Scholar
  11. Gutterman, J. U., McBride, C., Freireich, E. J., Mavligit, G., Frei, E., Hersh, E. M.: Active immunotherapy with BCG for recurrent malignant melanoma. Lancet 1, 1208 (1973)Google Scholar
  12. Holmes, E. C., Eilber, F. R., Morton, D. L.: Immunotherapy of malignancy in humans. J. Amer. Med. Assoc. 232, 1052 (1975)Google Scholar
  13. Jondal, M., Pross, H.: Surface markers on human B and T lymphocytes. VI. Cytotoxicity against cell lines as a functional marker for lymphocyte subpopulations. Int. J. Cancer 25, 596 (1975)Google Scholar
  14. Kaplan, M. E., Clark, C.: An improved rosetting assay for detection of human T-lymphocytes. J. Immunol. Methods 5, 131 (1974)Google Scholar
  15. Mathé, G.: Current status of immunotherapy of human cancers: leukaemias, lymphomas, solid tumours. Drugs 8, 411 (1974)Google Scholar
  16. Minden, P., Sharpton, T. R., McClatchy, J. K.: Shared antigens between human malignant melanoma cell lines and Mycobacterium bovis (BCG). J. Immunol. 116, 1407 (1976)Google Scholar
  17. Mokyr, M. B., Mitchell, M. S.: Activation of lymphoid cells by BCG in vitro. Cell. Immunol. 15, 264 (1975)Google Scholar
  18. Muchmore, A. V., Nelson, D. L., Kerchner, H., Blaese, R. M.: A reappraisal of the effector cells mediating mitogen induced cellular cytotoxicity. Cell. Immunol. 19, 78 (1975)Google Scholar
  19. Mukherji, B., Basses, D., Flowers, A., Binder, S. C., Nathanson, L.: Variables and specificity of in vitro lymphocyte-mediated cytotoxicity in human melanoma. Cancer Res. 35, 3721 (1975)Google Scholar
  20. Peter, H. H., Pavie-Fischer, J., Fridman, W. H., Aubert, C., Cesarini, J.-P., Roubin, R., Kourilsky, F. M.: Cell-mediated cytotoxicity in vitro of human lymphocytes against a tissue culture melanoma cell line (1GR3). J. Immunol. 115, 539 (1975)Google Scholar
  21. Solliday, S., Bach, F. H.: Cytotoxicity: specificity after in vitro sensitization. Science 170, 1406 (1970)Google Scholar
  22. Steele, G., Sjögren, H. O., Stadenberg, I.: In vitro cell mediated immune reactions of melanoma and colorectal carcinoma patients demonstrated by long-term 51chromium assays. Int. J. Cancer 17, 27 (1976)Google Scholar
  23. Sulit, H. L., Golub, S. H., Irie, R. F., Gupta, R. K., Grooms, G. A., Morton, D. L.: Human tumour cells grown in fetal serum and human serum: influences on the tests for lymphocyte cytotoxicity, serum blocking and serum arming effects. Int. J. Cancer 17, 461 (1976)Google Scholar
  24. Svedmyr, E., Jondal, M., Leibold, W.: Stimulation of normal lymphocytes with autologous lymphoid cell lines: properties of derived killer cells. Scand. J. Imm. 4, 721 (1975)Google Scholar
  25. Toh, B. H., Roberts-Thomson, I. C., Mathews, J. D., Whittingham, S., Mackay, I. R.: Depression of cell-mediated immunity in old age and the immunopathic diseases, lupus erythematosus, chronic hepatitis and rheumatoid arthritis. Clin. Exp. Immunol. 14, 193 (1973)Google Scholar
  26. de Vries, J. E., Cornain, S., Rümke, P.: Cytotoxicity of non-T versus T lymphocytes from melanoma patients and healthy donors on short and long-term cultured melanoma cells. Int. J. Cancer 14, 427 (1974)Google Scholar
  27. de Vries, J. E., Rümke, P.: Tumor-associated cytotoxicity superimposed on “spontaneous” cytotoxicity in melanoma patients. Int. J. Cancer 17, 182 (1976)Google Scholar
  28. West, W. H., Cannon, G. B., Kay, H. D., Bonnard, G. D., Herberman, R. B.: Natural cytotoxic reactivity of human lymphocytes against a myeloid cell line: characterization of effector cells. J. Immunol. 118, 355 (1977)Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • A. S. Coates
    • 1
  • M. Crawford
    • 1
  1. 1.Clinical Research UnitThe Walter and Eliza Hall Institute of Medical Research and the Royal Melbourne HospitalVictoriaAustralia

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