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Planta

, Volume 187, Issue 4, pp 438–444 | Cite as

Purification of iron superoxide dismutase from the cyanobacterium Anabaena cylindrica Lemm. and localization of the enzyme in heterocysts by immunogold labeling

  • A. Canini
  • P. Civitareale
  • S. Marini
  • M. Grilli Caiola
  • G. Rotilio
Article

Abstract

Iron superoxide dismutase (Fe-SOD; EC 1.15.1.1) was isolated from the nitrogen-fixing cyanobacterium Anabaena cylindrica Lemm. Polyacrylamide gel electrophoresis separated the purified protein into three closely running, enzymatically active bands. The molecular weight of the enzyme was estimated by gel filtration to be about 40 kDa. Polyclonal antibodies were produced by immunization of rabbits with the isolated enzyme, and were purified on a column of protein A-Sepharose. The Fe-SOD antibody reacted with the purified Fe-SOD and also specifically recognized the protein in extracts of A. cylindrica. In the extracts, anti-Fe-SOD did not cross-react with Mn-SOD, an enzyme which belongs to an SOD class displaying high homology of primary and three-dimensional structure with respect to Fe-SOD. Iron superoxide dismutase was localized in heterocysts by immunogold labeling and transmission electron microscopy. These results are the first in-situ evidence for the presence of SOD in the cells specialized for nitrogenase activity.

Key words

Anabaena Heterocyst Iron-superoxide dismutase (immunogold labeling) 

Abbreviations

ELISA

enzyme-linked immunosorbent assay

SDS

sodium dodecyl sulfate

SOD

superoxide dismutase

PAGE

polyacrylamide gel electrophoresis

pI

isoelectric point

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • A. Canini
    • 1
  • P. Civitareale
    • 1
  • S. Marini
    • 2
  • M. Grilli Caiola
    • 1
  • G. Rotilio
    • 1
  1. 1.Dipartimento di BiologiaII Università di Roma ‘Tor Vergata’RomaItaly
  2. 2.Dipartimento di Medicina SperimentaleII Università di Roma ‘Tor Vergata’RomaItaly

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