Planta

, Volume 195, Issue 2, pp 309–312 | Cite as

The cotyledon: A superior system for studies of leaf development

  • Hirokazu Tsukaya
  • Tomohiko Tsuge
  • Hirofumi Uchimiya
Rapid Communications

Abstract

The development of leaves in dicotyledonous plants is poorly understood because the division and expansion of cells occur at the same time and in the same positions. Our analysis has revealed that the growth of the cotyledons of Arabidopsis thaliana (L.) Heynh. depends only on the process of cell expansion. Thus, in this system, the roles of the expansion and division of cells in leaf development can be separated from each other. Analyzing the cotyledon, as a model leaf, we determined that a narrow-leaved mutant of Arabidopsis, angustifolia (an), has a defect in the polarity of the cell-expansion process. The defect was only manifested as the reduced width of blades of cotyledons, an observation which indicates that the direction of expansion of cotyledons is controlled by at least two genetically distinct pathways, one of which is regulated by the Angustifolia gene.

Key words

Arabidopsis (angustifolia mutant) Cotyledon Leaf morphology Model leaf Mutant (Arabidopsis

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Dale, J.E. (1988) The control of leaf expansion. Annu. Rev. Plant Physiol. Plant Mol. Biol. 39, 267–295Google Scholar
  2. Evans, M.W. (1940) Developmental morphology of the growing point of the shoot and the inflorescence in grasses. J. Agric. Res. 61, 481–520Google Scholar
  3. Guerrant, E.O. Jr. (1982) Neotenic evolution of Delphinium nudicaule (Ranunculaceae): A hummingbird-pollinated larkspur. Evolution 36, 699–712Google Scholar
  4. Hara, N. (1957) On the types of the marginal growth in dicotyledonous foliage leaves. Bot. Mag. Tokyo 70, 108–114Google Scholar
  5. Hara, N. (1959) Marginal growth of leaves. Nature 183, 1409–1410Google Scholar
  6. Imaichi, R., Kato, M. (1992) Comparative leaf development of Osmunda lancea and O. japonica (Osmundaceae): heterochronic origin of rheophytic stenophylly. Bot. Mag. Tokyo 105, 199–213Google Scholar
  7. Jeune, B. (1987) A model for the morphogenesis stage during leaf growth in dicotyledons. Biol. Zentralb. 106, 615–626Google Scholar
  8. Kato, M., Imaichi, R. (1991) Leaf anatomy of tropical fern rheophytes, with its evolutionary and ecological implications. Can. J. Bot. 70, 165–174Google Scholar
  9. Koornneef M., Dellaert, L.W.M., van der Veen, J.H. (1982) EMS-and radiation-induced mutation frequencies at individual loci in Arabidopsis thaliana (L.) Heynh. Mutat. Res. 93, 109–123Google Scholar
  10. Lee-Chen, S., Steinitz-Sears, L.M. (1967) The location of linkage groups in Arabidopsis thaliana. Can. J. Genet. Cytol. 9, 381–384Google Scholar
  11. Mansfield, S.G., Briarty, L.G. (1992) Cotyledon cell development in Arabidopsis thaliana during reserve deposition. Can. J. Bot. 70, 151–164Google Scholar
  12. McHale, N.A. (1993) LAM-1 and FAT genes control development of the leaf blade in Nicotiana sylvestris. Plant Cell 5, 1029–1038Google Scholar
  13. Meyerowitz, E.M., Pruitt, R.E. (1985) Arabidopsis thaliana and plant molecular genetics. Science 229, 1214–1218Google Scholar
  14. Poethig, R.S., Sussex, I.M. (1985) The developmental morphology and growth dynamics of the tobacco leaf. Planta 165, 158–169Google Scholar
  15. Rédei, G.P. (1962) Single locus heterosis. Z. Vererbungs. 93, 164–170Google Scholar
  16. Sinnott, E.W. (1958) The genetic basis of organic form. Ann. N.Y. Acad. Sci. 71, 1223–1233Google Scholar
  17. Smith, L.G., Hake, S. (1992) The initiation and determination of leaves. Plant Cell. 4, 1017–1027Google Scholar
  18. Tsukaya, H., Naito, S., Rédei, G.P., Komeda, Y. (1993) A new class of mutations in Arabidopsis thaliana, acaulis1, affecting the development of both inflorescences and leaves. Development 118, 751–764Google Scholar
  19. Tsukaya, H., Ohshima, T., Naito, S., Chino, M., Komeda, Y. (1991) Sugar-dependent expression of the CHS-A gene for chalcone synthase from petunia in transgenic Arabidopsis thaliana. Plant Physiol. 97, 1414–1421Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Hirokazu Tsukaya
    • 1
  • Tomohiko Tsuge
    • 1
  • Hirofumi Uchimiya
    • 1
  1. 1.Institute of Molecular and Cellular BiosciencesThe University of TokyoTokyoJapan

Personalised recommendations