, Volume 195, Issue 2, pp 309–312 | Cite as

The cotyledon: A superior system for studies of leaf development

  • Hirokazu Tsukaya
  • Tomohiko Tsuge
  • Hirofumi Uchimiya
Rapid Communications


The development of leaves in dicotyledonous plants is poorly understood because the division and expansion of cells occur at the same time and in the same positions. Our analysis has revealed that the growth of the cotyledons of Arabidopsis thaliana (L.) Heynh. depends only on the process of cell expansion. Thus, in this system, the roles of the expansion and division of cells in leaf development can be separated from each other. Analyzing the cotyledon, as a model leaf, we determined that a narrow-leaved mutant of Arabidopsis, angustifolia (an), has a defect in the polarity of the cell-expansion process. The defect was only manifested as the reduced width of blades of cotyledons, an observation which indicates that the direction of expansion of cotyledons is controlled by at least two genetically distinct pathways, one of which is regulated by the Angustifolia gene.

Key words

Arabidopsis (angustifolia mutant) Cotyledon Leaf morphology Model leaf Mutant (Arabidopsis


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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Hirokazu Tsukaya
    • 1
  • Tomohiko Tsuge
    • 1
  • Hirofumi Uchimiya
    • 1
  1. 1.Institute of Molecular and Cellular BiosciencesThe University of TokyoTokyoJapan

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