Planta

, Volume 195, Issue 2, pp 175–181 | Cite as

Extensin gene expression is induced by mechanical stimuli leading to local cell wall strengthening in Nicotiana plumbaginifolia

  • Christine Tiré
  • Riet De Rycke
  • Marc De Loose
  • Dirk Inzé
  • Marc Van Montagu
  • Gilbert Engler
Article

Abstract

Nicotiana plumbaginifolia Viv. harbors a single extensin gene, although related hydroxyproline-rich sequences are present in the genome. Northern analysis showed that the gene is highly expressed in roots and to a lesser extent in stems. Expression in leaves is low but mRNA levels are increased upon infection with the incompatible bacterium Pseudomonas syringae. Extensin transcript levels in leaves were slightly enhanced after wounding and salicylic acid treatment. In-situ hybridization experiments showed high accumulation of extensin mRNA in cells which, at certain stages of development, require reinforcement of their cell walls. The cortical cells in stem nodes and roots, which are put under severe mechanical stress by adjacent developing tissues, tend to express the gene to high levels. Immunolocalization of the extensin protein in stems and roots demonstrated a close association of the protein with lignin deposition. Mature tissues contained more extensin than younger tissues. The extensin promoter was fused to the β-glucuronidase gene.

Key words

Developmental regulation Extensin (gene expression) Gene expression (extensin) Nicotiana (extensin) Protein localization 

Abbreviation

HRGP

hydroxyproline-rich glycoprotein

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Christine Tiré
    • 1
  • Riet De Rycke
    • 1
  • Marc De Loose
    • 1
  • Dirk Inzé
    • 2
  • Marc Van Montagu
    • 1
  • Gilbert Engler
    • 2
  1. 1.Laboratorium voor GeneticaUniversiteit GentGentBelgium
  2. 2.Laboratoire Associé de l'Institut National de la Recherche Agronomique (France)Universiteit GentGentBelgium
  3. 3.Rijksstation voor PlantenveredelingMerelbeke-LembergeBelgium

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