Planta

, Volume 192, Issue 1, pp 118–124 | Cite as

Maize embryo germination

I. Cell cycle analysis
  • Elena I. Georgieva
  • Gerardo López-Rodas
  • A. Hittmair
  • H. Feichtinger
  • G. Brosch
  • Peter Loidl
Article

Abstract

The cell-cycle progression of germinating embryos of maize (Zea mays L.) was studied from 0 to 72 h after the start of imbibition using DNA flow cytometry on isolated nuclei, and analyses of thymidine kinase activity, histone biosynthesis and levels of proliferating cell nulcear antigen (PCNA). At the start of germination, 75% of the cells were in G1, but this population had decreased to 25% by 72 h. The concomitant increase of cells in S-phase did not occur continuously, but stepwise, indicating that during germination most of the cells enter S-phase as a partially synchronized population. Within the initial 60 h of embryo germination the cells passed through one S-phase; the start and duration of this period of replicative DNA synthesis was further substantiated by the analysis of S-phase-associated events, the biosynthesis of core histones and the enzyme activity of thymidine kinase, which both began to increase at about 12 h after the start of differentiation. Thymidine kinase fluctuated periodically during germination with a transient maximum at 30 h and a second peak at 72 h; histone biosynthesis was not detectable until 12 h after the start of germination. The levels of PCNA protein closely resembled the pattern of thymidine kinase during germination. Together with the cytometric data this allows a clear assignment of cell cycle events to different times of embryo differentiation.

Key words

Cell cycle Differentiation Flow cytometry Histone Proliferating cell nuclear antigen Zea 

Abbreviation

PCNA

proliferating-cell nuclear antigen

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Elena I. Georgieva
    • 1
  • Gerardo López-Rodas
    • 1
  • A. Hittmair
    • 2
  • H. Feichtinger
    • 2
  • G. Brosch
    • 1
  • Peter Loidl
    • 1
  1. 1.Department of MicrobiologyUniversity of InnsbruckInnsbruckAustria
  2. 2.Department of PathologyUniversity of InnsbruckInnsbruckAustria
  3. 3.Institute of Genetics, Department of Molecular GeneticsBulgarian Academy of SciencesSofiaBulgaria
  4. 4.Department of Biochemistry and Molecular Biology, Faculties of SciencesUniversity of ValenciaBurjassot (Valencia)Spain

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