Journal of Biomolecular NMR

, Volume 3, Issue 6, pp 715–720 | Cite as

Minimizing the effects of radio-frequency heating in multidimensional NMR experiments

  • Andy C. Wang
  • Ad Bax
Short Communications


Application of radio-frequency power in multidimensional NMR experiments can significantly increase the sample temperature compared to that of the surrounding gas flow. Radio-frequency heating effects become more severe at higher magnetic field strengths and ionic strengths. The effects are particularly noticeable for experiments that utilize 1H and/or 13C isotropic mixing and broadband decoupling. If radio-frequency power is applied during the systematically increasing evolution period t1, the sample temperature can change with t1 and thereby cause line-shape distortions. Such distortions are easily avoided by ensuring that the average radio-frequency power remains constant during the entire experiment.


Radio-frequency heating Line-shape distortion Multidimensional NMR Temperature Decoupling Isotropic mixing 


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Copyright information

© ESCOM Science Publishers B.V 1993

Authors and Affiliations

  • Andy C. Wang
    • 1
  • Ad Bax
    • 1
  1. 1.Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney DiseasesNational Institutes of HealthBethesdaUSA

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