Planta

, Volume 198, Issue 1, pp 64–69 | Cite as

Cytosolic ascorbate peroxidase from Arabidopsis thaliana L. is encoded by a small multigene family

  • Maria Santos
  • Helene Gousseau
  • Clare Lister
  • Christine Foyer
  • Gary Creissen
  • Philip Mullineaux
Article

Abstract

A second cytosolic ascorbate peroxidase (cAPX; EC 1.11.1.11) gene from Arabidopsis thaliana has been characterised. This second gene (designated APX1b) maps to linkage group 3 and potentially encodes a cAPX as closely related to that from other dicotyledonous species as to the other member of this gene family (Kubo et al, 1993, FEBS Lett 315: 313–317; here designated APX1a), which maps to linkage group 1. In contrast, the lack of sequence similarity in non-coding regions of the genes implies that they are differentially regulated. Under non-stressed conditions only APX1a is expressed. APX1b was identified during low-stringency probing using a cDNA coding for pea cAPX which, in turn, was recovered from a cDNA library by immunoscreening with an antiserum raised against tea plastidial APX (pAPX). No pAPX cDNAs were recovered, despite the antiserum displaying specificity for pAPX in Western blots.

Key words

Arabidopsis Ascorbate peroxidase (cytosolic, plastidial) Multigene family Oxidative stress Pisum 

Abbreviations

ATG

methionine translation initiation codon

bp

base pair

cAPX

cytosolic ascorbate peroxidase

pAPX

plastidial ascorbate peroxidase

RFLP

restriction fragment length polymorphism

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Maria Santos
    • 1
  • Helene Gousseau
    • 2
  • Clare Lister
    • 1
  • Christine Foyer
    • 2
  • Gary Creissen
    • 1
  • Philip Mullineaux
    • 1
  1. 1.John Innes CentreColneyUK
  2. 2.Laboratoire de MetabolismeINRA, Centre de Recherches de VersaillesVersaillesFrance
  3. 3.M.I.R., University of CalgaryCalgaryCanada
  4. 4.Institute of Grassland and Environmental Research, Plas GogerddanAberystwythUK

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