Virchows Archiv

, Volume 429, Issue 1, pp 37–42

Losses at 3p common deletion sites in subtypes of kidney tumours: histopathological correlations

  • Piotr Hadaczek
  • Jacek Podolski
  • Aleksandra Toloczko
  • Grzegorz Kurzawski
  • Jan Lubinski
  • Andrzej Sikorski
  • Pamela Rabbitts
  • Kay Huebner
Original Articles

Abstract

Deletions of the short arm of chromosome 3 (3p) have been recognized as characteristic features of clear cell renal cell carcinomas (clear cell RCC). We analysed 55 clear-cell RCCs and 30 non-clear-cell kidney tumours (10 papillary and 7 chromophobic RCCs, 11 oncocytomas and 2 collecting duct carcinomas) in loss of heterozygosity (LOH) studies using microsatellite markers for previously observed regions of common deletions on 3p in kidney tumours (3p25, 3p21.3, 3p14.2 and 3p12-13). Alterations were found in all 55 cases of clear-cell RCCs at two to four of the 3p regions. Extensive losses were not found in non-clear-cell tumours except for collecting duct carcinomas; 1 of 10 papillary RCCs showed interstitial deletion limited to a single 3p21.3 locus. LOH analyses using microsatellite markers for regions of common deletions at 3p may be of value in differential diagnosis of kidney tumours.

Key words

Kidney tumour classification 3p Loss of heterozygosity 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Anglard P, Tory K, Brauch H, Weiss GH, Latif F, Merino MJ, Lerman MI, Zbar B, Linehan WM (1991) Molecular analysis of genetic changes in the origin and development of renal cell carcinoma. Cancer Res 51:1071–1077Google Scholar
  2. 2.
    Anglard P, Trahan E, Liu S, Latif F, Merino MJ, Lerman MI, Zbar B, Linehan WM (1992) Molecular characterization of human renal cell carcinoma cell lines. Cancer Res 52: 348–356Google Scholar
  3. 3.
    Cawkwell L, Bell SM, Lewis FA, Dixon MF, Taylor GR, Quirke P (1993) Rapid detection of allele loss in colorectal tumours using microsatellites and fluorescent DNA technology. Br J Cancer 67:1262–1267Google Scholar
  4. 4.
    El-Naggar A, Batsakis J, Wang G, Lee M-S (1993) PCR-based RELP screening of the commonly deleted 3p loci in renal cortical neoplasms. Diagn Mol Pathol 2:269–276Google Scholar
  5. 5.
    Foster K, Prowse A, Berg A van den, Fleming S, Hulsbeek MMF, Crossey PA, Richards FM, Cairns P, Affara NA, Ferguson-Smith MA, Buys CHCM, Maher ER (1994) Somatic mutations of the von Hippel-Lindau disease tumour sppressor gene in nonfamilial clear-cell renal carcinoma. Hum Mol Genet 3:2169–2173Google Scholar
  6. 6.
    Füzesi L, Cober M, Mittermayer C (1992) Collecting duct carcinoma: cytogenetic characterization. Histopathology 21:155–160Google Scholar
  7. 7.
    Gnarra JR, Tory K, Weng Y, Schmidt L, Wei MH, Li H, Latif F, Liu S, Chen F, Duh F-M, Lubensky I, Duan DR, Florence C, Pozzatti R, Walther MM, Bander NH, Grossman HB, Brauch H, Pomer S, Brooks JD, Issacs WB, Lerman MI, Zbar B, Linehan WM (1994) Mutations of the VHL tumour suppressor gene in renal carcinoma. Nat Genet 7:85–90Google Scholar
  8. 8.
    Kovacs G, Erlandsson R, Boldog F, Ingvarsson S, Muller-Brechlin R, Klein G, Sumegi J (1988) Consistent chromosome 3p deletion and loss of heterozygosity in renal cell carcinoma. Proc Natl Acad Sci USA 85:1571–1575Google Scholar
  9. 9.
    Kovacs A, Störkel S, Thoenes W, Kovacs G (1992) Mitochondrial and chromosomal DNA alterations in human chromophobe renal cell carcinoma. J Pathol (Lond) 167:273–277Google Scholar
  10. 10.
    LaForgia S, Lasota J, Latif F, Boghosian-Sell L, Kastury K, Ohta M, Druck T, Atchison L, Cannizzaro L, Barnea G, Schlesinger J, Modi W, Kuzmin I, Tory K, Zbar B, Croce CM, Lerman M, Huebner K (1993) Detailed genetic and physical map of the 3p chromosome region surrounding the familial RCC chromosome translocation, t(3;8)(p14.2;q24.1). Cancer Res 53:3118–3124Google Scholar
  11. 11.
    Lubinski J, Chosia M, Kotanska K, Huebner K (1988) Genotypic analysis of DNA isolated from fine needle aspiration biopsies. Anal Quant Cytol Histol 10:383–390Google Scholar
  12. 12.
    Lubinski J, Hadaczek P, Podolski J, Toloczko A, Sikorski A, McCue P, Druck T, Huebner K (1994) Common regions of deletion in chromosome regions 3p12 and 3p14.2 in primary clear-cell renal carcinomas. Cancer Res 54:3710–3713Google Scholar
  13. 13.
    Morita R, Ishikawa J, Tsutsumi M, Hikiji K, Tsukada Y, Kamidono S, Maeda S, Nakamura Y (1991) Allelotype of renal cell carcinoma. Cancer Res 51:820–823Google Scholar
  14. 14.
    Naylor S, Buys CHCM, Carritt B (1994) Report of the Fourth International Workshop on Human Chromosome 3 Mapping. Cytogenet Cell Genet 65:1–50Google Scholar
  15. 15.
    Ogawa O, Kakehi Y, Ogawa K, Koshiba M, Sugiyama T, Yoshida O (1991) Allelic loss at chromosome 3p characterizes clear-cell phenotype renal cell carcinoma. Cancer Res 51:949–953Google Scholar
  16. 16.
    Presti J, Reuter V, Cordon-Cordo C, Mazumdar M, Fair WR, Jhanwar SC (1993) Allelic deletions in renal tumours: histopathological correlations. Cancer Res 53:5780–5783Google Scholar
  17. 17.
    Robson C, Churchil B, Anderson W (1969) The results of radical nephrectomy for renal cell carcinomas. J Urol 101:297–301Google Scholar
  18. 18.
    Roche J, Whisenant E, Boldog F, Loeb D, Vance JM, Drabkin H (1994) Dinucleotide repeats flanking the renal cell carcinoma breakpoint at 3p14.2. Hum Mol Genet 3:215Google Scholar
  19. 19.
    Tallini G (1994) Analysis of nuclear and mitochondrial DNA alterations in thyroid and renal oncocytic tumour. Cytogenet Cell Genet 66:250–259Google Scholar
  20. 20.
    Thoenes W, Störkel S, Rumpelt H (1986) Histopathology and classification of renal cell tumours (adenomas, oncocytomas and carcinomas): the basic cytological and histopathological elements and their use for diagnosis. Pathol Res Pract 181:125–143Google Scholar
  21. 21.
    Van den Berg A, Hulsbeek MMF, Jong D de, Kok K, Veldhuis PMJF, Roche J, Buys CHCM (1996) Major role for a 3p21 region and lack of involvement of the t(3;8) breakpoint region in the development of renal cell carcinoma suggested by loss of heterozygosity analysis. Genes Chromosome Cancer 15:64–72Google Scholar
  22. 22.
    Van den Berg E, Hout A van der, Oosterhuis J, Störkel S, Dijkhuizen T, Dam A, Zweers HMM, Mensink HJA, Buys CHCM, Jong B de (1993) Cytogenetics analysis of epithelial renal-cell tumours: relationship with a new histopathological classification. Int J Cancer 55:223–227Google Scholar
  23. 23.
    Van der Hout AH, Berg E van den, Vlies P van der, Dijkhuizen T, Störkel S, Oosterhuis JW, Jong B de, Buys CHCM (1993) Loss of heterozygosity at the short arm of chromosome 3 in renal-cell cancer correlates with the cytological tumour type. Int J Cancer 53:353–357Google Scholar
  24. 24.
    Vogelstein B, Fearon ER, Hamilton SR, Kern SE, Preisinger AC, Leppert M, Nakamura Y, White R, Smits AMM, Bos JL (1988) Genetic alterations during colorectal-tumour development. N Engl J Med 319:525–532PubMedGoogle Scholar
  25. 25.
    Yamakawa K, Morita R, Takahashi E, Hori T, Ishikawa J, Nakamura Y (1991) A detailed deletion mapping of the short arm of chromosome 3 in sporadic renal cell carcinoma. Cancer Res 51:4707–4711Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Piotr Hadaczek
    • 1
  • Jacek Podolski
    • 1
  • Aleksandra Toloczko
    • 1
  • Grzegorz Kurzawski
    • 1
  • Jan Lubinski
    • 1
  • Andrzej Sikorski
    • 2
  • Pamela Rabbitts
    • 3
  • Kay Huebner
    • 4
  1. 1.Department of Genetics and PathologyPomeranian Medical AcademySzczecinPoland
  2. 2.Department of UrologyPomeranian Medical AcademySzczecinPoland
  3. 3.MRC Clinical Oncology and Radiotherapeutics UnitMedical Research Council CentreCambridgeUK
  4. 4.Jefferson Cancer InstituteJefferson Medical CollegePhiladelphiaUSA

Personalised recommendations