Planta

, Volume 185, Issue 1, pp 17–26

A Chlamydomonas reinhardtii low-starch mutant is defective for 3-phosphoglycerate activation and orthophosphate inhibition of ADP-glucose pyrophosphorylase

  • Steven Ball
  • Thérèse Marianne
  • Léon Dirick
  • Marc Fresnoy
  • Brigitte Delrue
  • André Decq
Article

Abstract

A low-starch mutant accumulating less than 5% of wild-type amounts was isolated after X-ray mutagenesis of Chlamydomonas reinhardtii cells. The recessive st-1-1 defect segregated as a single mendelian mutation through meiosis, and led to a severe decrease in starch accumulation under all culture conditions tested, whether in the light or in darkness. Adenosine 5′-diphosphoglucose pyrophosphorylase (in the absence of 3-phosphoglycerate), starch synthase, phosphoglucomutase, phosphorylase and starch-branching enzyme were all characterized and shown to be unaffected by the mutation. However, ADP-glucose pyrophosphorylase in the mutant had its sensitivity to activation by 3-phosphoglycerate lowered dramatically and became less responsive to orthophosphate. Our results are consistent both with a mutation in a structural gene of a multisubunit enzyme or in a regulatory gene responsible for switching ADP-glucose pyrophosphorylase from a 3-phosphoglycerate-insensitive to a 3-phosphoglycerate-sensitive form. These results provide definite proof of the in-vivo requirement for 3-phosphoglycerate activation to obtain substantial starch synthesis in plants. The conclusions hold both for synthesis from CO2 in the light or from exogenous organic carbon sources in darkness. A model is presented in which the existence of a 3-phosphoglycerate gradient explains localized starch synthesis around the pyrenoid of lower plants.

Key words

ADP-glucose pyrophosphorylase Chlamydomonas Phosphorylase Pyrenoid Starch 

Abbreviations

glucose-1-P

glucose-1-phosphate

glucose-6-P

glucose-6-phosphate

Pi

orthophosphate

3PGA

3-phospho-glycerate

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Steven Ball
    • 1
  • Thérèse Marianne
    • 1
  • Léon Dirick
    • 1
  • Marc Fresnoy
    • 1
  • Brigitte Delrue
    • 1
  • André Decq
    • 1
  1. 1.Laboratoire de Chimie BiologiqueUniversité des Sciences et Techniques de Lille Flandres-ArtoisVilleneuve d'Ascq cedexFrance

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