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Planta

, Volume 189, Issue 3, pp 461–467 | Cite as

Shift in carbon flow and stimulation of amino-acid turnover induced by nitrate and ammonium assimilation in Anacystis nidulans

  • Tomás Coronil
  • Catalina Lara
  • Miguel G. Guerrero
Article

Abstract

The influence of nitrate and ammonium assimilation on the flow of recently fixed carbon has been determined in intact Anacystis nidulans cells actively fixing CO2. Assimilation of nitrate or ammonium resulted in substantial increases in the incorporation of carbon into acid-soluble metabolites, the magnitude of the effect being dependent on the irradiance. The radiolabel in sugar phosphate was virtually unaffected by nitrogen assimilation, whereas that in organic acids and, in particular, in amino acids was markedly increased. Enhancement of carbon incorporation into amino acids induced by nitrogen assimilation was not accompanied by parallel increases in the size of the amino acid pools. This resulted in an appreciable increase of the specific radioactivity of most amino acids under conditions of nitrogen assimilation. The data indicate that nitrate and ammonium assimilation induce an enhancement of carbon flow through the glycolytic and the tricarboxylic-acid pathways to oxaloacetate and α-ketoglutarate, as well as a stimulation of amino-acid turnover. These effects were more pronounced at saturating irradiance.

Key words

Amino acid turnover Anacystis Carbonnitrogen interactions Carbon assimilation Nitrate and ammonium assimilation Photosynthetic metabolism 

Abbreviations

Chl

chlorophyll

GS

glutamine synthetase (EC 6.3.1.2.)

GOGAT

ferredoxin-dependent glutamate synthase (EC 1.4.7.1.)

α-KG

α-ketoglutarate

OAA

oxaloacetate

PEP

phosphoenolpyruvate

Pyr

pyruvate

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Tomás Coronil
    • 1
  • Catalina Lara
    • 1
  • Miguel G. Guerrero
    • 1
  1. 1.Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla-CSICSevillaSpain

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