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Plant Cell Reports

, Volume 15, Issue 3–4, pp 287–292 | Cite as

Agrobacterium tumefaciens-mediated transformation of broccoli (Brassica oleracea var. italica) and cabbage (B. oleracea var. capitata)

  • Timothy D. Metz
  • Ram Dixit
  • Elizabeth D. Earle
Article

Abstract

Transgenic broccoli (Brassica oleracea var. italica) was produced by two Agrobacterium tumefaciens-mediated transformation methods. One used flowering stalk explants from mature plants; the other used hypocotyl and petiole explants from in vitro-grown seedlings. Several hundred transformants containing a Bacillus thuringiensis ∂-endotoxin gene (CryIA(c)-type) and the neomycin phosphotransferase gene were recovered. Rooted transformants were obtained in as little as 3 months using seedling explants. Transgenic cabbage was also obtained by the seedling explant method. Parameters important for high efficiency regeneration and transformation rates included use of a tobacco nurse cell layer, sealing of petri dishes with a porous surgical tape instead of Parafilm, preculture of seedling explants and appropriate length of co-cultivation with Agrobacterium. Advantages and disadvantages of each transformation procedure are discussed.

Keywords

Shoot Regeneration Transgenic Shoot Petiole Explants Timentin Seedling Explants 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Timothy D. Metz
    • 1
  • Ram Dixit
    • 1
  • Elizabeth D. Earle
    • 1
  1. 1.Dept. of Plant BreedingCornell UniversityIthacaUSA

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