, Volume 193, Issue 2, pp 181–185 | Cite as

An hydraulic interpretation of rapid, long-distance wound signalling in the tomato

  • M. Malone
  • J-J. Alarcon
  • Luigia Palumbo


Localised wounding causes rapid and systemic induction of proteinase inhibitors in tomato (Lycopersicon esculentum L.). The signalling system which coordinates this response is not known. Recent work has shown that systemic hydraulic signals are transmitted from wound sites in tomato, but that these cannot by themselves induce proteinase inhibitors. Here, it is demonstrated that the hydraulic signal is nevertheless an essential requirement for the systemic induction of proteinase inhibitors by localised treatments. It is also shown that mass flows similar to those associated with the wound-induced hydraulic signal, can convey a variety of solutes rapidly throughout the shoot. It is concluded that long-distance wound signalling in the tomato occurs by xylem transmission of soluble elicitors in the mass flows induced by wounding.

Key words

Defence response (systemic) Hydraulic signal Lycopersicon (wounding) Proteinase-inhibitor-inducing factor Proteinase inhibitor Wounding 



proteinase inhibitors


proteinase-inhibitor-inducing factors


rhamnogalacturonan 1


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  1. Barlow, H.W.B. (1979) Sectorial patterns in leaves on fruit tree shoots produced by radioactive assimilates and solutions. Ann. Bot. 43, 593–602Google Scholar
  2. Baydoun, E.A.-H., Fry, S.C. (1985) The immobility of pectic substances in injured tomato leaves and its bearing on the identity of the wound hormome. Planta 165, 269–276Google Scholar
  3. Bennett, A.B., Sweger, B.L., Spanswick, R.M. (1984) Sink to source translocation in soybean. Plant Physiol. 74, 434–436Google Scholar
  4. Beraud, J., Brun, A., Feray, A., Hourmant, A., Penot, M. (1992) Transport of 14C-putrescine in potato plantlets. Biochem. Phys. Pflanzen 88, 169–176Google Scholar
  5. Boari, F., Malone, M. (1993) Rapid and systemic hydraulic signals are induced by localised wounding in a wide range of species. J. Exp. Bot. 44, 741–746Google Scholar
  6. Bradford, K.J., Yang, S.F. (1980) Xylem transport of ACC, an ethylene precursor, in waterlogged tomato plants. Plant Physiol. 65, 322–326Google Scholar
  7. Bradshaw, H.D., Hollick, J.B., Parsons, T.J., Clarke, H.R.G., Gordon, M.P. (1989) Wound-responsive genes in poplar encode proteins similar to sweet potato sporamins and legume kunitz trypsin inhibitors. Plant Mol. Biol. 14, 51–59Google Scholar
  8. Burbano, J.L., Pizzolato, T.D., Morey, P.R., Berlin, J.D. (1976) An application of the prussian blue technique to a light microscope study of water movement in transpiring leaves of cotton (Gossypium hirsutum L.). J. Exp. Bot. 27, 134–144Google Scholar
  9. Canny, M.J. (1990) What becomes of the transpiration stream? New Phytol. 114, 341–368Google Scholar
  10. Chessin, M., Zipf, A.E. (1990) Alarm systems in higher plants. Bot. Rev. 56, 193–235Google Scholar
  11. Gowing, D.J.G., Jones, H.G., Davies, W.J. (1993) Xylem-transported abscisic acid: the relative importance of its mass and its concentration in the control of stomatal aperture. Plant Cell Environ. 16, 453–459Google Scholar
  12. Graham, J.S., Hall, G., Pearce, G., Ryan, C.A. (1986) Regulation of synthesis of proteinase inhibitors I and II mRNAs in leaves of wounded tomato plants. Planta 169, 399–405Google Scholar
  13. Keil, M., Sanchez-Serrano, J.J., Willmitzer, L. (1989) Both wound-inducible and tuber-specific expression are mediated by the promoter of a single member of the proteinase inhibitor II gene family. EMBO J. 8, 303–306Google Scholar
  14. Malone, M. (1992) Kinetics of wound-induced hydraulic signals and variation potentials in wheat seedlings. Planta, 187, 505–510Google Scholar
  15. Malone, M. (1993) Hydraulic signals. Phil. Trans. Roy. Soc London Ser. B 341, 33–39Google Scholar
  16. Malone, M., Palumbo, L., Boari, F., Monteleone, M., Jones, H.G. (1994) The relationship between wound-induced proteinase inhibitors and hydraulic signals in tomato seedlings. Plant Cell Environ. 17, 81–87Google Scholar
  17. Malone, M., Stanković, B. (1991) Surface potentials and hydraulic signals in wheat leaves following localised wounding by heat. Plant Cell Environ. 14, 431–436Google Scholar
  18. McGurl, B., Pearce, G., Orozco-Cardenas, M., Ryan, C.A. (1992) Structure, expression, and antisense inhibition of the systemin precursor gene. Science 255, 1570–1573Google Scholar
  19. McNeil, M., Darvill, A.G., Albersheim, P. (1980) Structure of plant cell walls X. Rhamnogalacturonan I, a structurally complex pectic polysaccharide in the walls of suspension-cultured sycamore cells. Plant Physiol. 66, 1128–1134Google Scholar
  20. Minchin, P.E.H., McNaughton, G.S. (1987) Xylem transport of recently fixed carbon within lupin. Aust. J. Plant Physiol. 14, 325–329Google Scholar
  21. Nooden, L.D., Murray, B.J. (1982) Transmission of the monocarpic senescence signals via the xylem in soybean. Plant Physiol. 69, 754–756Google Scholar
  22. Oertli, J.J. (1993) Effect of cavitation on the status of water in plants. In: Water transport in plants under climatic stress, pp. 27–40, Borghetti, M., Grace, J., Raschi, A. eds., Cambridge University Press, UKGoogle Scholar
  23. Pearce, G., Johnson, S., Ryan, C.A. (1993) Purification and characterisation from tobacco leaves of six small, wound-inducible, proteinase inhibitors of the potato inhibitor II family Plant Physiol. 102, 639–644Google Scholar
  24. Peña-Cortes, H., Sánchez-Serrano, J.J., Rocha-Sosa, M., Willmitzer, L. (1988) Systemic induction of proteinase-inhibitor-II gene expression in potato plants by wounding. Planta 174, 84–89Google Scholar
  25. Ryan, C.A. (1974) Assay and biochemical properties of the proteinase inhibitor-inducing factor, a wound hormone. Plant Physiol. 54, 328–332Google Scholar
  26. Ryan, C.A. (1992) The search for the proteinase-inhibitor inducing factor, PIIF. Plant Mol. Biol. 19, 123–133Google Scholar
  27. Ryan, C.A., Bishop, P., Pearce, G., Darvill, A.G., McNeil, M., Alber-sheim, P. (1981) A sycamore cell wall polysaccharide and a chemically related tomato leaf polysaccharide possess similar proteinase inhibitor-inducing activities. Plant Physiol. 68, 616–618Google Scholar
  28. Tepfer, M., Taylor, I.E.P. (1981) The permeability of plant cell walls as measured by gel filtration chromatography. Science 213, 761–763Google Scholar
  29. Van de Pol, P.A., Marcelis, L.F.M. (1988) Apical application of aqueous solutions to roses via flower tubes — a technique with possibilities. Sci. Hort. 34, 123–129Google Scholar
  30. Wildon, D.C., Thain, J.F., Minchin, P.E.H., Grubb, I.R., Reilly, A.J., Skipper, Y.D., Doherty, H.M., O'Donnell, P.J., Bowles, D.J. (1992) Electrical signalling and systemic proteinase inhibitor in the wounded plant. Nature 360, 62–65Google Scholar
  31. Wolterbeek, H.T., Van Luipen, J., De Bruin, M. (1984) Non-steady state xylem transport of fifteen elements into the tomato leaf as measured by gamma-ray spectroscopy: a model. Physiol. Plant. 61, 599–606Google Scholar
  32. Zanetti, A., Beauvais, F., Huet, J-C., Pernollet, J.C. (1992) Movement of elicitins, necrosis-inducing proteins secreted by Phytophthora sp., in tobacco. Planta 187, 163–170Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • M. Malone
    • 1
  • J-J. Alarcon
    • 1
  • Luigia Palumbo
    • 1
  1. 1.HRI WellesbourneWarwicksUK

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