Planta

, Volume 193, Issue 2, pp 155–162 | Cite as

Metabolic engineering of glycine betaine synthesis: plant betaine aldehyde dehydrogenases lacking typical transit peptides are targeted to tobacco chloroplasts where they confer betaine aldehyde resistance

  • Bala Rathinasabapathi
  • Kent F. McCue
  • Douglas A. Gage
  • Andrew D. Hanson
Article

Abstract

Certain higher plants synthesize and accumulate glycine betaine, a compound with osmoprotectant properties. Biosynthesis of glycine betaine proceeds via the pathway choline → betaine aldehyde → glycine betaine. Plants such as tobacco (Nicotiana tabacum L.) which do not accumulate glycine betaine lack the enzymes catalyzing both reactions. As a step towards engineering glycine betaine accumulation into a non-accumulator, spinach and sugar beet complementary-DNA sequences encoding the second enzyme of glycine-betaine synthesis (betaine aldehyde dehydrogenase, BADH, EC 1.2.1.8) were expressed in tobacco. Despite the absence of a typical transit peptide, BADH was targeted to the chloroplast in leaves of transgenic plants. Levels of extractable BADH were comparable to those in spinach and sugar beet, and the molecular weight, isoenzyme profile and Km for betaine aldehyde of the BADH enzymes from transgenic plants were the same as for native spinach or sugar beet BADH. Transgenic plants converted supplied betaine aldehyde to glycine betaine at high rates, demonstrating that they were able to transport betaine aldehyde across both the plasma membrane and the chloroplast envelope. The glycine betaine produced in this way was not further metabolized and reached concentrations similar to those in plants which accumulate glycine betaine naturally. Betaine aldehyde was toxic to non-transformed tobacco tissues whereas transgenic tissues were resistant due to detoxification of betaine aldehyde to glycine betaine. Betaine aldehyded ehydrogenase is therefore of interest as a potential selectable marker, as well as in the metabolic engineering of osmoprotectant biosynthesis.

Key words

Nicotiana (transgenic) Betaine aldehyde dehydrogenase Chloroplast targeting Glycine betaine Selectable marker 

Abbreviations

BADH

betaine aldehyde dehydrogenase

bp

base pairs

FAB-MS

fast atom bombardment-mass spectrometry

GAPDH

NADP-linked glyceraldehyde-3-phosphate dehydrogenase

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Bala Rathinasabapathi
    • 1
  • Kent F. McCue
    • 2
  • Douglas A. Gage
    • 3
  • Andrew D. Hanson
    • 1
  1. 1.Institut de Recherche en Biologie Végétale de l'Université de MontréalMontréalCanada
  2. 2.USDA-ARSAlbanyUSA
  3. 3.Biochemistry DepartmentMichigan State UniversityEast LansingUSA

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