Advertisement

Medical Microbiology and Immunology

, Volume 179, Issue 5, pp 225–235 | Cite as

Effects of succinylated concanavalin A on infectivity and syncytial formation of human immunodeficiency virus

  • Toshio Matsui
  • Susumu Kobayashi
  • Osamu Yoshida
  • Shin-ichi Ishii
  • Yukichi Abe
  • Naoki Yamamoto
Original Investigations

Abstract

The effects of various lectins on the infectivity of human immunodeficiency virus (HIV) type 1 was investigated. Among the 25 lectins investigated, 2 types of concanavalin A (Con A) and 3 types of phytohemagglutinin were found to inhibit HIV infection. Succinylated Con A (S-Con A) efficiently blocked HIV-induced formation of syncytia in a coculture of MOLT-4 cells and blocked cell-free infection by HIV of MT-4 cells. The HIV-binding study revealed that S-Con A only partially inhibited viral binding to cells, although the control Leu-3a monoclonal antibody strongly inhibited it. When S-Con A was added to cultures after the initiation of viral adsorption, the number of HIV antigen-positive cells that developed depended on the time interval before addition of the compound. S-Con A inhibited HIV infection even after viral binding to cells at 0 °C and further incubation at 37 °C for 1 day. These data suggest that S-Con A inhibited mainly the fusion process rather than viral binding to cells in exerting its anti-HIV activity.

Keywords

Monoclonal Antibody Human Immunodeficiency Virus Human Immunodeficiency Virus Infection Concanavalin Fusion Process 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abe Y, Iwabuchi M, Ishii S (1971) Multiple forms in the subunit structure of concanavalin A. Biochem Biophys Res Commun 45:1271–1278Google Scholar
  2. Barre-Sinoussi F, Chermann JC, Rey F, Nugeyre MT, Chamaret S, Gruest J, Dauguet C, Axler-Blin C, Vezinet-Brun F, Rouzioux C, Montagnier L (1983) Isolation of a T lymphotropic retrovirus from a patient at risk for AIDS. Science 220:868–871Google Scholar
  3. Dalgleish AG, Beverly PEL, Clapham PR, Crawford DH, Greaves MP, Weiss RA (1984) The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus. Nature 312:763–767Google Scholar
  4. Ezekowitz RAB, Kuhlman M, Groppman JE, Byrn RA (1989) A human serum mannose-binding protein inhibits in vitro infection by the human immunodeficiency virus. J Exp Med 169:185–196Google Scholar
  5. Garrity P, Szelc C, Paquentte C, McEvoy M, Milette R, Adler R (1982) Inhibition of herpes simplex virus replication by succinyl concanavalin A. Antimicrob Agents Chemother 21:450–455Google Scholar
  6. Gollins SW, Porterfield JS (1986) A new mechanism for the neutralization of enveloped viruses by antiviral antibody. Nature 321:244–246Google Scholar
  7. Harada S, Koyanagi Y, Yamamoto N (1985a) Infection of HTLV-III/LAV in HTLV-I carrying cells MT-2 and MT-4 and application in a plaque assay. Science 299:563–565Google Scholar
  8. Harada S, Koyanagi Y, Yamamoto N (1985b) Infection of human T lymphotropic virus type-I (HTLV-I)-bearing MT-4 cells with HTLV-III (AIDS virus): chronological studies of early events. Virology 146:272–281Google Scholar
  9. Ishii S, Abe Y, Tanaka I, Saito M (1984) Alteration of quaternary stucture and biological activity of concanavalin A. J Protein Chem 3:63–71Google Scholar
  10. Klatzmann D, Barre-Sinoussi F, Nugeyre MT, Dauguet G, Vilmer E, Griscelli C, Brun-Vezinet F, Rouzioux C, Gluckman JC, Chermann JC, Montagnier L (1984) Selective tropism of lymphadenopathy associated virus (LAV) for helper-inducer T lymphocytes. Science 225:59–63Google Scholar
  11. Kowalski M, Potz J, Basiripour L, Dorfan T, Goh WC, Terwilliger E, Dayton A, Rosen C, Haseltine W, Sodroski J (1987) Functional regions of the envelope glycoprotein of human immunodeficiency virus type 1. Science 237:1351–1355Google Scholar
  12. Koyanagi Y, Harada S, Yamamoto N (1986) Establishment of a high production system for AIDS retroviruses with a human T-leukemic cell line MOLT-4. Cancer Lett 30:299–310Google Scholar
  13. Levy JA, Hoffman AD, Kramer SM, Landis TA, Shimabukuro JM (1984) Isolation of lymphocytopathic retroviruses from San Francisco patients with AIDS. Science 225:840–842Google Scholar
  14. Lifson JD, Coutre S, Huang E, Engleman E (1986a) Role of envelope glycoprotein carbohydrate in human immunodeficiency virus (HIV) infectivity and virus-indued cell fusion. J Exp Med 164:2101–2106Google Scholar
  15. Lifson JD, Reyes GR, McGrath MS, Stein GS, Engleman E (1986b) AIDS retrovirus induced cytopathology: giant cell formation and involvement of CD4 antigen. Science 232:1123–1127Google Scholar
  16. Maddon PJ, Dalgleish AG, McDougal SJ, Clapham PR, Weiss RA, Axel R (1987) The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain. Cell 47:333–348Google Scholar
  17. Nakashima H, Yoshida O, Baba M, De Clercq E, Yamamoto N (1989) Anti-HIV activity of dextran sulphate as determined under different experimental conditions. Antiviral Res 11:223–246Google Scholar
  18. Oda Y, Kasai K, Ishii S (1981) Studies on the specific interaction of concanavalin A and saccharides by affinity chromatography. Application of quantitative affinity chromatography to a multivalent system. J Biochem 89:285–296Google Scholar
  19. Popovic M, Sarngadharan MG, Read E, Gallo RC (1984) Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS. Science 224:497–500Google Scholar
  20. Robey WG, Safai B, Oroszlan S, Arthur LO, Gonda MA, Gallo RC, Fishcinger PF (1985) Characterization of envelope and core structural gene products of HTLV-III with sera from AIDS patients. Science 288:593–595Google Scholar
  21. Saito M, Takaku F, Hayashi M, Tanaka I, Abe Y, Nagai Y, Ishii (1983) A role of valency of concanavalin A and its chemically modified deriatives in lymphocyte activation. Monovalent monomeric concanavalin A derivative can stimulate lymphocyte blastoid transformation. J Biol Chem 258:7499–7505Google Scholar
  22. Tanabe A, Nakashima H, Yoshida O, Yamamoto N, Tenmyo O, Oki T (1988) Inhibition effect of new antibiotic, pradimicin A on infectivity, cytopathic effect and replication of human immunodeficiency virus in vitro. J Antibiot (Tokyo) 41:1708–1710Google Scholar
  23. Tanabe-Tochikura A, Tochikura TS, Yoshida O, Oki T, Yamamoto N (1990) Pradimicin A inhibition of human immunodeficiency virus: attenuation by mannan. Virology 176:467–473Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Toshio Matsui
    • 1
  • Susumu Kobayashi
    • 1
  • Osamu Yoshida
    • 1
  • Shin-ichi Ishii
    • 2
  • Yukichi Abe
    • 2
  • Naoki Yamamoto
  1. 1.Department of Virology and ParasitologyYamaguchi University School of MedicineYamaguchiJapan
  2. 2.Department of BiochemistryFaculty of Pharmaceutical Sciences, Hokkaido UniversityHokkaidoJapan

Personalised recommendations