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Medical Microbiology and Immunology

, Volume 182, Issue 6, pp 329–334 | Cite as

Density heterogeneities of hepatitis C virus in human sera due to the binding of β-lipoproteins and immunoglobulins

  • R. Thomssen
  • S. Bonk
  • A. Thiele
Original Investigations

Abstract

Heterogeneities in the density of hepatitis C virus RNA-carrying material (HCV-RNA-CM) found in human sera (1.03–1.20 g/cm3) are attributed to the binding of low-density lipoproteins and/or of IgG. In some sera HCV-RNA-CM seems to be nearly totally bound to β-lipoproteins and cannot be precipitated by anti-IgG (γ); in others more than 95% of HCV-RNA-CM is bound to IgG and cannot be precipitated by anti-β-lipoprotein. Furthermore, there are sera from which HCV-RNA-CM can be completely be precipitated by either anti-β-lipoprotein or anti-IgG (γ), pointing to a binding of the two serum proteins to the same HCV-RNA-CM. There are other sera from which HCV-RNA-CM can be partially precipitated by the one or the other antiserum, leaving behind fractions, which are bound to β-lipoprotein or to IgG. HCV-RNA-CM cannot be precipitated from some sera either by anti-β-lipoprotein or by anti-IgG (γ).

Keywords

Hepatitis Serum Protein Human Seron Density Heterogeneity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Agnello V, Chung RT, Kaplan LM (1992) A role for hepatitis C virus infection in type II cryoglobulinemia. N Engl J Med 327:1490–1495Google Scholar
  2. Bradley DW, Krawczynski K, Beach MJ, Purdy MA (1991a) Non-A, non B hepatitis: towards the discovery of hepatitis C and E viruses. Semin Liver Dis 11:128–146Google Scholar
  3. Bradley DW, McCaustland K, Krawczynski K, Spelbring J, Humphrey C, Cook EH (1991b) Hepatitis C virus: buoyant density of the factor VIII-derived isolate in sucrose. J Med Virol 34:206–208Google Scholar
  4. Brown MS, Goldstein JL (1986) A receptor-mediated pathway for cholesterol homeostasis. Science 232:34–47Google Scholar
  5. Choo Q-L, Weiner AJ, Overby LR, Kuo G, Houghton M, Bradley DW (1990) Hepatitis C virus: the major causative agent of viral non-A, non-B hepatitis. Br Med Bull 46:423–441Google Scholar
  6. Clarke DH, Casals J (1958) Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg 7:561–573Google Scholar
  7. Farci P, Alter HJ, Govindarajan S, Wong DC, Engle R, Lesniewski RR, Mushahwar IK, Resai SM, Miller RH, Ogata N, Purcell HP (1992) Lack of protective immunity against reinfection with hepatitis C virus. Science 258:135–140Google Scholar
  8. Hellström UB, Sylvan SPE (1986) Regulation of the immune response to hepatitis B virus and human serum albumin. I. Hepatitis B surface antigen-induced secretion of antibodies with specificity for human serum albumin in hepatitis B-immune donors in vitro. Scand J Immunol 23, 545–553Google Scholar
  9. Hijikata M, Shimizu YK, Kato H, Iwamoto A, Shih JW, Alter HJ, Parcell RH, Yoshikura H (1993) Equilibrium centrifugation studies of hepatitis C virus: Evidence for circulating immune complexes J Virol 67:1953–1958Google Scholar
  10. Hubry MA, Schauf U (1978) Transfusion-related short incubation hepatitis in hemophiliacs. JAMA 240:1355–1357Google Scholar
  11. Kaboth U and the DFG study group (1980) Kooperative prospektive Studie “Akute Virushepatitis DFG”. Verh Dtsch Ges Inn Med 86:749–756Google Scholar
  12. Krone B, Lenz A, Heermann K-H, Seifer M, Lu X and Gerlich WH (1990) Interaction between hepatitis B surface proteins and monomeric human serum albumin. Hepatology 11:1050–1056Google Scholar
  13. Laufs R, Thomssen R (1968) Inhibition der Rubellavirus-Hämagglutination. Eigenschaften eines in fetalem Kälberserum vorkommenden Inhibitors der Rubellavirus-Hämagglutination in vitro. Arch Ges Virusforsch 24:181–191Google Scholar
  14. Mann JJ, Rosen RD, Lehrich JR, Kasel JA (1967) The effect of kaolin in immunoglobulins. An improved technique to remove the non-specific serum inhibitor of reovirus hemagglutination. J Immunol 98:1136–1142Google Scholar
  15. Mosley JW, Redeker AG, Feinstone SM, Purcell RH (1977) Multiple hepatitis viruses in multiple attacks of acute viral hepatitis. N Engl J Med 296:75–78Google Scholar
  16. Norkrans G, Frösner G, Hermodsson S, Iwarson S (1980) Multiple hepatitis attacks in drug addicts. JAMA 243:1056–1061Google Scholar
  17. Pfeifer U, Thomssen R, Legler K, Gerlich W, Weinmann E, Klinge O (1980) Experimental nonA, nonB hepatitis: four types of cytoplasmic alteration in hepatocytes of infected chimpanzees Virchows Arch [B] 33:233–243Google Scholar
  18. Seitz H (1993) Eigenschaften von recombinanten Produkten der hypothetischen Nucleocapsid-Genregion des Hepatitis C-Virus. PhD Thesis, University of Göttingen, GöttingenGoogle Scholar
  19. Thomssen R, Bonk S, Propfe C, Heermann KH, Köchel HG, Uy A (1992) Association of hepatitis C virus in human sera with β-lipoprotein. Med Microbiol Immunol 181:293–300Google Scholar
  20. Weiner AJ, Geysen HM, Christopherson C, Hall JE, Mason TJ, Saracco G, Bonino F, Crawford K, Marion CD, Crawford KA, Brunetto M, Barr PJ, Miyamura T, McHutschinson J, Houghton M (1992) Evidence for immune selection of hepatitis C virus (HCV) putative envelope glycoprotein variants: potential role in chronic HCV infections. Proc Natl Acad Sci USA 89:3468–3472Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • R. Thomssen
    • 1
  • S. Bonk
    • 1
  • A. Thiele
    • 1
  1. 1.Department of Medical MicrobiologyUniversity of GöttingenGöttingenGermany

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